Jing Huang, Ph.D.
Jing  Huang, Ph.D.
Investigator
Head, Cancer and Stem Cell Epigenetics Section

Dr. Huang is interested in studying the roles of p53 in the stress response of stem cells. His current research focuses on embryonic stem cells and mesenchymal stem cells, which are relevant to sarcomas. Dr. Huang hopes to gain insights from his studies that may contribute to p53-based therapy for both stem cell diseases and cancer. To achieve these goals, Dr. Huang’s laboratory employs biochemical, molecular, epigenetic, and genomic approaches as well as mouse models.

Areas of Expertise
1) embryonic stem cells 2) mesenchymal stem cells 3) p53 4) epigenetics 5) genomics 6) sarcoma

Contact Info

Jing Huang, Ph.D.
Center for Cancer Research
National Cancer Institute
Building 37, Room 3140A
Bethesda, MD 20892-4258
301-496-2202
huangj3@mail.nih.gov

The research interest of the Cancer and Stem Cell Epigenetics Section focuses on studying the roles of p53 in stem cells. Specifically, we are investigating the roles of p53 downstream targets in the regulation of stem cell differentiation and apoptosis and how these roles are related to the developmental roles and tumor suppressive functions of p53. We have two complementary stem cell models: embryonic stem cells (ESCs) and mesenchymal stromal/stem cells (MSCs).

Project 1: Study p53-mediated DNA damage responses of ESCs. ESCs can develop into many different cell types and have huge potential in clinical application. However, it is under appreciated how ESCs maintain their genome stability responding to DNA damage insults. We aim to address this question by studying how p53 regulates the DNA damage responses of ESCs. We and others have found that p53 plays important roles in the regulation of ESC differentiation after DNA damage. To achieve this, p53 down-regulates the transcription of many ES cell critical genes (Li et al., Cell Stem Cell, 2015; Zhang et al., Cell Cycle, 2013; Li et al., Molecular Cell, 2012; Lee et al., PNAS, 2010). Since ESCs are derived from blastocysts, our study may also shed new light on the emerging developmental roles of p53.

Project 2: Investigate the roles of p53 in MSCs (also called bone marrow-derived MSCs or BMSCs). MSCs are thought to be one of the cell-of-origins of osteosarcoma. p53 plays important roles in suppressing osteosarcoma. Thus, we hope to gain new insights into the osteosarcoma suppressive functions of p53 by studying the actions of p53 in MSCs (He et al., Stem Cells, 2015).

To achieve our goals, we use various classical and cutting-edge techniques including molecular biology (such as CRISPR), biochemistry (e.g., proteomics), mouse genetics, genomics (e.g., ChIP-seq and RNA-seq), and systems biology.

Scientific Focus Areas:
Cancer Biology, Cell Biology, Genetics and Genomics, Molecular Biology and Biochemistry, Stem Cell Biology
  1. Li M, Gou H, Tripathi BK, Huang J, Jiang S, Dubois W, Waybright T, Lei M, Shi J, Zhou M, Huang J
    Cell Stem Cell. In press: 2015. [ Journal Article ]
  2. He Y, de Castro LF, Shin MH, Dubois W, Yang HH, Jiang S, Mishra PJ, Ren L, Gou H, Lal A, Khanna C, Merlino G, Lee M, Robey PG, Huang J
    Stem Cells. 33: 1304-1319, 2015. [ Journal Article ]
  3. Zhang X, He Y, Lee K, Dubois W, Li Z, Wu X, Kovalchuk A, Zhang W, Huang J.
    Cell Cycle. 12: 1279-91, 2013. [ Journal Article ]
  4. Li M, He Y, Dubois W, Wu X, Shi J, Huang J.
    Mol Cell. 46: 30-42, 2012. [ Journal Article ]
  5. Lee KH, Li M, Michalowski AM, Zhang X, Liao H, Chen L, Xu Y, Wu X, and Huang J.
    Proc Natl Acad Sci U S A. 107: 69-74, 2010. [ Journal Article ]

Dr. Huang received his BS in Biochemistry from Peking University. He studied the estrogen receptor signaling in breast cancer with Drs. Robert Bambara and Mesut Muyan at the University of Rochester (NY) and received his PhD in 2004. After his postdoctoral training in cancer epigenetics with Dr. Shelley Berger at the Wistar Institute, he joined the Laboratory of Cancer Biology and Genetics as a tenure-track Principal Investigator in October, 2008. Dr. Huang's section is currently study the roles of p53 in stem cells. Dr. Huang won a NCI Director's Innovation Award with Dr. Jianxin Shi (DCEG) in 2011.

Position Number of Positions Contact E-mail Contact Name Contact Phone
Postdoctoral Fellow 1

huangj3@mail.nih.gov

Jing Huang, Ph.D. 301.496.2202
Name Position
Yunlong He Ph.D. Postdoctoral Fellow (Visiting)
Shunlin Jiang M.D. Research Assistant
Mangmang Li Ph.D. Postdoctoral Fellow (Visiting)
Eryney Marrogi Special Volunteer
Min Hwa Shin Ph.D. Postdoctoral Fellow (Visiting)

Research

The research interest of the Cancer and Stem Cell Epigenetics Section focuses on studying the roles of p53 in stem cells. Specifically, we are investigating the roles of p53 downstream targets in the regulation of stem cell differentiation and apoptosis and how these roles are related to the developmental roles and tumor suppressive functions of p53. We have two complementary stem cell models: embryonic stem cells (ESCs) and mesenchymal stromal/stem cells (MSCs).

Project 1: Study p53-mediated DNA damage responses of ESCs. ESCs can develop into many different cell types and have huge potential in clinical application. However, it is under appreciated how ESCs maintain their genome stability responding to DNA damage insults. We aim to address this question by studying how p53 regulates the DNA damage responses of ESCs. We and others have found that p53 plays important roles in the regulation of ESC differentiation after DNA damage. To achieve this, p53 down-regulates the transcription of many ES cell critical genes (Li et al., Cell Stem Cell, 2015; Zhang et al., Cell Cycle, 2013; Li et al., Molecular Cell, 2012; Lee et al., PNAS, 2010). Since ESCs are derived from blastocysts, our study may also shed new light on the emerging developmental roles of p53.

Project 2: Investigate the roles of p53 in MSCs (also called bone marrow-derived MSCs or BMSCs). MSCs are thought to be one of the cell-of-origins of osteosarcoma. p53 plays important roles in suppressing osteosarcoma. Thus, we hope to gain new insights into the osteosarcoma suppressive functions of p53 by studying the actions of p53 in MSCs (He et al., Stem Cells, 2015).

To achieve our goals, we use various classical and cutting-edge techniques including molecular biology (such as CRISPR), biochemistry (e.g., proteomics), mouse genetics, genomics (e.g., ChIP-seq and RNA-seq), and systems biology.

Scientific Focus Areas:
Cancer Biology, Cell Biology, Genetics and Genomics, Molecular Biology and Biochemistry, Stem Cell Biology

Publications

  1. Li M, Gou H, Tripathi BK, Huang J, Jiang S, Dubois W, Waybright T, Lei M, Shi J, Zhou M, Huang J
    Cell Stem Cell. In press: 2015. [ Journal Article ]
  2. He Y, de Castro LF, Shin MH, Dubois W, Yang HH, Jiang S, Mishra PJ, Ren L, Gou H, Lal A, Khanna C, Merlino G, Lee M, Robey PG, Huang J
    Stem Cells. 33: 1304-1319, 2015. [ Journal Article ]
  3. Zhang X, He Y, Lee K, Dubois W, Li Z, Wu X, Kovalchuk A, Zhang W, Huang J.
    Cell Cycle. 12: 1279-91, 2013. [ Journal Article ]
  4. Li M, He Y, Dubois W, Wu X, Shi J, Huang J.
    Mol Cell. 46: 30-42, 2012. [ Journal Article ]
  5. Lee KH, Li M, Michalowski AM, Zhang X, Liao H, Chen L, Xu Y, Wu X, and Huang J.
    Proc Natl Acad Sci U S A. 107: 69-74, 2010. [ Journal Article ]

Biography

Dr. Huang received his BS in Biochemistry from Peking University. He studied the estrogen receptor signaling in breast cancer with Drs. Robert Bambara and Mesut Muyan at the University of Rochester (NY) and received his PhD in 2004. After his postdoctoral training in cancer epigenetics with Dr. Shelley Berger at the Wistar Institute, he joined the Laboratory of Cancer Biology and Genetics as a tenure-track Principal Investigator in October, 2008. Dr. Huang's section is currently study the roles of p53 in stem cells. Dr. Huang won a NCI Director's Innovation Award with Dr. Jianxin Shi (DCEG) in 2011.

Positions

Position Number of Positions Contact E-mail Contact Name Contact Phone
Postdoctoral Fellow 1

huangj3@mail.nih.gov

Jing Huang, Ph.D. 301.496.2202

Team

Name Position
Yunlong He Ph.D. Postdoctoral Fellow (Visiting)
Shunlin Jiang M.D. Research Assistant
Mangmang Li Ph.D. Postdoctoral Fellow (Visiting)
Eryney Marrogi Special Volunteer
Min Hwa Shin Ph.D. Postdoctoral Fellow (Visiting)