Sukbir Kaur, Ph.D.
Exosomes or extracellular vesicles (EVs) are shed by all kinds of cells, and recent research has shown that exosomes play a major role in cell-cell communication and also are associated with disease progression such as cancer metastasis. Dr. Kaur discovered that CD47, which is known as the “don’t eat me” signal and which is upregulated in many cancers, is also present on exosomes. Her major interest is to explore the role of exosome-associated CD47 in breast and prostate cancers.
1) exosomes/extracellualr vesicles, 2) cancer stem cells, 3) miRNA biogenesis,
4) RNA sequencing data analysis, 5) molecular and cell biology, 6) small non-coding RNAs
Cancer Stem Cell Research
Cancer stem cells have been implicated in the spread of cancer cells and in poor progonses for many cancers. Our lab is interested in identifying roles for CD47 and SIRP alpha receptors in human breast cancer stem cells and in understanding the regulation of these receptors and the genes associated with cancer stem cell markers in vitro and in vivo.
Non-coding RNA Biogenesis: Non-coding RNA (ncRNA) has been implicated in development activities such as embryogenesis, differentiation, growth control, and programmed cell death. Altered expression of miRNA leads to disarrangement of cellular mechanisms in diseases such as cancer. Changes in expression profiles of ncRNA have been identified as potential biomarkers for cancer. Understanding the novel role of CD47 receptors in the regulation of ncRNA biogenesis using global ncRNA-Seq and ncRNA microarray expression levels in normal and cancer exosomes from various cell lines such as breast, colon, ovary, small lung carcinomas, and melanomas is one area of interest.
RNA-Sequencing Bioinformatics: Next-generation sequencing data analysis is evolving rapidly and explores key scientific information buried in raw reads. We are interested in exploring RNA-Seq analysis and The Cancer Genome Atlas (TCGA) database to understand coding-non-coding RNAs using a wide variety of bioinformatics software.
Selected Key Publications
CD63, MHC class 1, and CD47 identify subsets of extracellular vesicles containing distinct populations of noncoding RNAs.Sci Rep. 8(1): 2577, 2018. [ Journal Article ]
A function-blocking CD47 antibody suppresses stem cell and EGF signaling in triple-negative breast cancer.Oncotarget. 7(9): 10133-52, 2016. [ Journal Article ]
CD47-dependent immunomodulatory and angiogenic activities of extracellular vesicles produced by T cells.Matrix Biol. 37: 49-59, 2014. [ Journal Article ]
Thrombospondin-1 signaling through CD47 inhibits self-renewal by regulating c-Myc and other stem cell transcription factors.Sci Rep. 3: 1673, 2013. [ Journal Article ]
- J Biol Chem. 285(50): 38923-32, 2010. [ Journal Article ]
Dr. Kaur was a predoctoral fellow with Dr. Ramani Ramchandran at NCI. She then took a postdoctoral fellowship in the Genome Technology Branch at the National Human Genome Research Institute (NHGRI) where she developed an interest in miRNAs and non-coding RNAs. Later, she joined Dr. David Roberts' group, the Biochemical Pathology Section, eventually becoming a staff scientist. Dr. Kaur has received many awards, including the Director's Innovation Award, the NIH Mentoring Award, and technology transfer awards.
Her research interests include exploration of differential regulation of CD47 in cancer versus normal stem cells as well as cell-cell communication via exosomes and their functional role in cancer. In addition to this, she is interested in miRNAs and non-coding RNAs, RNA-Seq data analysis, and the downstream bioinformatics analyses.
She serves on peer review panels for PLoS One, Scientific Reports and JOVE. Additionally, she has been an active member of the Staff Scientist/Staff Clinician (SSSC) Organization, since she became a staff scientist in 2015. She plays an active role in organizing Professional Development Day for the Professional Development Committee.