Subhadra Banerjee, Ph.D.
Dr. Banerjee is a Research Biologist in Flow Cytometry Core Facility in the Laboratory of Genome Integrity. The Laboratory provides a state-of-the-art multiparameter flow cytometry and cell sorting for NCI Laboratories. Dr. Banerjee started working in the FACS Core Facility, Building 37 in March of 2007 as a full-time biologist with extensive experience in cancer biology and immunohistochemistry.
As a biologist, Dr. Banerjee performs high-speed cell sorting in tube, plate and slide (based on the investigator’s need) on the 3 Laser (11 to 13 Parameters) FACSAria using complicated cell sorting software (Diva 8). Dr. Banerjee also participates actively in developing new criteria and improving methodology and procedures for the FACS Core facility users.
1) flow cytometry, 2) immunohistochemistry, 3) cancer biology, 4) cell biology, 5) laser capture microscopy
Dr. Banerjee routinely uses Aria sorters to sort primary murine keratinocytes, melanocytes, thyroid, lung, liver, prostate, and mammary cells, tumor xenografts, cell lines transfected with GFP, dsRed reporters, potential cancer stem cells from cell lines and primary tumors based on surface antigen expression, SP (side population), or aldehyde dehydrogenase expression. She also performs plate-sorting for cloning and is responsible for setting up slide-sorting procedure for single cell PCR using the Advalytix equipment for some LCBG and CCBB researchers .
Besides cell sorting, Dr. Banerjee's responsibilities are to provide training to the new users, instrument operation (start up and shut down) of the facilities bench top flow cytometers, data acquisition and data analysis using various software. She also assist users running experiments, provide expertise in selecting methodology and procedure to meet experimental objectives, optimizing experimental protocol in order to obtain the most complete and accurate assessment, and troubleshoot problems to assure accuracy of experimental results.
Expression of the B-cell receptor component CD79a on immature myeloid cells contributes to their tumor promoting effects.PLoS One. 8: e76115, 2013. [ Journal Article ]
- Genes Dev. 25: 275-286, 2011. [ Journal Article ]
Proteomic analysis of stage I endometrial cancer tissue: identification of proteins associated with oxidative processes and inflammation.Gynecol Oncol. 121: 586-594, 2011. [ Journal Article ]
Dr.Banerjee received her Ph.D from School of Life Science, Visva Bharati University, India. She then joined the Department of Biochemistry and Molecular Biology, Johns Hopkins School of Hygiene as a postdoctoral fellow. After a few years of postdoctoral experience she became a Research Associate in the Department of Biochemistry and Molecular Biology, Johns Hopkins University.
Dr. Banerjee came to National Institute of Mental Health as a Biologist on February of 2001. After five years of service at National Institute of Mental Health, she moved to Gynecologic Disease Center at Walter Reed Army Medical Center on March of 2006. Dr. Banerjee came back to National Cancer Institute on March of 2007 as a Research Biologist at NCI CCR Flow Cytometry Core Facility in Building 37 at NIH.