Sriram Subramaniam, Ph.D.
We develop and apply novel methods in 3D electron microscopy and image processing to determine structures of a variety of macromolecular assemblies of fundamental biomedical interest. Main areas of current interest include: structural analysis of membrane transporters and dynamic protein complexes involved in signaling and metabolism, structure and structural variations of enveloped virus glycoproteins and their applicability to vaccine design, 3D subcellular imaging with correlative light and electron microscopy to explore host-pathogen interactions, and the development of methods for high-resolution cryo-EM.
For more information, visit these websites:
Three-Dimensional Electron Microscopy at Molecular Resolution
For a more detailed description of our research program, please visit our High Resolution Electron Microscopy website at http://electron.nci.nih.gov.
The long-term mission of our research program is to obtain an integrated, quantitative understanding of cells and viruses at molecular resolution. We take an interdisciplinary approach to this problem by combining novel technologies for three-dimensional (3D) imaging with computational and cell biological tools. Our research efforts are presently focused on three areas: (1) determination of the structure and mechanisms underlying neutralization and cellular entry of HIV, (2) the development of automated, high-throughput workflows for structure determination of small, dynamic molecular complexes, and (3) the development of novel technologies for 3D imaging of cells and tissues, with particular emphasis on methods for understanding and diagnosing structural signatures of signal transduction and disease progression.
Selected Key Publications
Cryo-EM structures reveal mechanism and inhibition of DNA targeting by a CRISPR-Cas surveillance complex.Cell. 171(2): 414-426, 2017. [ Journal Article ]
- Nature. 537(7621): 567-571, 2016. [ Journal Article ]
- Cell. 165(7): 1698-1707, 2016. [ Journal Article ]
- Cell. 164: 747-756, 2016. [ Journal Article ]
- Science. 351: 871-5, 2016. [ Journal Article ]
Dr. Sriram Subramaniam received his Ph.D. in Physical Chemistry from Stanford University and completed postdoctoral training in the Departments of Chemistry and Biology at M.I.T. He is chief of the Biophysics Section in the Laboratory of Cell Biology at the Center for Cancer Research, National Cancer Institute. He holds a visiting faculty appointment at the Johns Hopkins University School of Medicine. His current work is focused on the development of advanced technologies for imaging macromolecular assemblies using 3D electron microscopy, and their application to address fundamental problems in HIV/AIDS and cancer research.
|Alberto Bartesaghi, Ph.D.||Associate Scientist|
|Brian Caffrey||Predoctoral Fellow (Visiting)|
|Sagar Chittori Ph.D.||Postdoctoral Fellow (Visiting)|
|Joe Darling Ph.D.||Postdoctoral Fellow (CRTA)|
|Jean-Philippe Demers Ph.D.||Postdoctoral Fellow (Visiting)|
|Lesley Ann Earl Ph.D.||Scientific Communications Editor (Contr.)|
|Veronica Falconieri||Illustrator (Contr.)|
|Joseph Finney||Engineer (Contr.)|
|Andrei Florescu||Data Manager (Contr.)|
|Reinhard Grisshammer, Ph.D.||Staff Scientist|
|Lisa Hartnell||Research Biologist|
|Martin Kessel Ph.D.||Special Volunteer|
|Doreen Matthies Ph.D.||Postdoctoral Fellow (Visiting)|
|Jana Ognjenovic Ph.D.||Postdoctoral Fellow (CRTA)|
|Erin Tran, Ph.D.||Staff Scientist|
|Andrew R. Wilbur||Postbaccalaureate Fellow (CRTA)|
|Xing Zhu Ph.D.||Postdoctoral Fellow (Visiting)|