Reinhard Grisshammer, Ph.D.
Dr. Grisshammer studies the structural mechanism of G protein-coupled receptor (GPCR) activation, in particular that of neuropeptide receptors. His work currently focuses on GPCRs in complex with signaling partners such as G protein or arrestin, by both X-ray crystallography and cryo-electron microscopy approaches.
1) membrane protein over-expression, 2) membrane protein purification,
3) 3D-crystallization, 4) G protein-coupled receptors (GPCRs), 5) pharmacology,
6) structure determination
Dr. Grisshammer’s research focuses on understanding the mechanistic aspects and structural changes which occur during the activation of G protein-coupled receptors (GPCRs). His structural work is complemented by pharmacological and biochemical approaches. Using the peptide hormone receptor for neurotensin as an example, X-ray structures in various signaling forms provided insight into how the binding of the peptide ligand on the outside of the receptor triggers conformational changes within the receptor to promote G protein coupling and thus activation. His current research centers on GPCRs in complex with signaling partners such as G protein or arrestin.
Selected Key Publications
- Nature. 490: 508-513, 2012. [ Journal Article ]
Optimising the combination of thermostabilising mutations in the neurotensin receptor for structure determination.Biochim. Biophys. Acta Biomembranes. 1828: 1293-1301, 2013. [ Journal Article ]
- The Journal of Physical Chemistry B. 119: 4917-4928, 2015. [ Journal Article ]
- Nature Communications. 6: 7895, 2015. [ Journal Article ]
- Scientific Reports. 6: 38564, 2016. [ Journal Article ]
Dr. Grisshammer received his Ph.D. degree in 1990 from the Johann-Wolfgang Goethe University in Frankfurt, Germany, where he worked with Hartmut Michel at the Max-Planck-Institute of Biophysics. Dr. Grisshammer did post-doctoral work with Richard Henderson at the Medical Research Council Laboratory of Molecular Biology, Cambridge, England, where he focused on structural work on G protein-coupled receptors (GPCRs). Dr. Grisshammer joined NIDDK in 2001 as a staff scientist, and NINDS in 2006 as an investigator, where his laboratory used a combination of structural and functional approaches to answer mechanistic questions regarding G protein-coupled receptors. In 2017, he transitioned to the Laboratory of Cell Biology at NCI as a staff scientist where he continues research on integral membrane proteins and GPCRs.