William G. Telford, Ph.D.
The Core provides a wide variety of state-of-the-art flow and image cytometry instrumentation. Full support is provided to users of this instrumentation, including initial planning of experiments, training on the appropriate instrumentation, assistance in data collection, subsequent data analysis, and final presentation of results.
1) flow and image cytometry
The NCI ETIB Flow Cytometry Facility provides advanced flow and imaging cytometry and cell sorting services to investigators in the Experimental Transplantation and Immunotherapy Branch, and to several other Branches in the NCI Center for Cancer Research. The Facility maintains a suite of ten basic and advanced flow and imaging cytometers, including six benchtop analyzers and three cell sorters. This group of instruments provides comprehensive experimental coverage, from the simplest one-color experiments to high-dimensional labeling experiments requiring up to 20 parameters. The Facility also maintains a laser scanning cytometer, allowing image cytometry of cell samples affixed to slides or culture dishes. This last technology is specialized and is not widely available at the NIH; the Facility has developed a special expertise in its use, and offers it to the entire NIH community regardless of institutional affiliation. Expert training and assistance are provided by experienced staff for all of this equipment, from initial experiment planning to experiment execution to reporting and publication of data. The NCI ETIB Flow Cytometry Facility is therefore a comprehensive scientific resource for ETIB and NCI scientific investigators.
Selected Key Publications
Stem cell side population analysis and sorting using DyeCycle violet.In: Current Protocols in Cytometry. Chapter 9: 2010. [ Book Chapter ]
- Stem Cells. 25(4): 1029-36, 2007. [ Journal Article ]
The minimal instrumentation requirements for Hoechst side population analysis: stem cell analysis on low-cost flow cytometry platforms.Stem Cells. 24(11): 2573-81, 2006. [ Journal Article ]
Discrimination of the Hoechst side population in mouse bone marrow with violet and near-ultraviolet laser diodes.Cytometry A. 57(1): 45-52, 2004. [ Journal Article ]
William Telford, Ph.D. received his Ph.D. in microbiology from Michigan State University in 1994, where his laboratory developed some of the earliest techniques for flow cytometric detection of apoptosis. He carried out his postdoctoral training in immunology at The University of Michigan Medical School, was appointed assistant scientist at the Hospital for Special Surgery – Weill Cornell University School of Medicine in New York City from 1997 to 1999. Dr. Telford was recruited as a Staff Scientist and manager of the ETIB Flow Cytometry Facility in 1999, and became an Associate Scientist in 2008.
Dr. Telford has over 20 years experience in flow cytometry. He is extensively involved in both national and international cytometry education programs, including the National Flow Cytometry Resource Flow Cytometry Workshops (as a sustaining faculty member) and the International Society for Advancement of Cytometry, where he has taught numerous courses, tutorials and workshops over the last 10 years. He has over 100 publications in the fields of immunology and cytometry, with over half of them as primary, corresponding or originating author. He is the co-founder and coordinator of the NIH Flow Cytometry Interest Group, and is a sustaining faculty member in the Indo-US Flow Cytometry Workshop program, teaching flow cytometry methods at many biomedical institutions in India. He maintains a small independent research and development program in the Facility aimed at both hardware and wetware development, particularly in the area of novel laser technology, and has published extensively in this area.
|Nga Hawk Ph.D.||Biologist|
|Veena Kapoor B.S.||Senior Research Assistant|