Our Science – Barr Website
Valarie A. Barr, Ph.D.
The Microscopy Core facility provides state-of-the-art equipment, advice and training to researchers with particular expertise in live cell imaging. Most of the research done in the Core facility focuses on the movement of proteins involved in signal transduction pathways. In collaboration with Dr. Samelson, we study the movements of proteins initially found in signaling complexes that form at activated T cell receptors. These studies use a model system where chimeric proteins tagged with variants of GFP are visualized in Jurkat T cells. These cells are activated either by contact with antibody-coated coverslips or by contact with superantigen pulsed Raji B cells. Using this system we have studied the formation of signaling complexes, the movement of proteins involved in reorganization of the actin cytoskeleton and the trafficking of SLP-76, an important adaptor protein involved in T cell activation.
This page was last updated on 3/18/2014.