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Our Science – Caplen Website

Natasha J. Caplen, Ph.D.

Selected Publications

1)  Tandle AT, Kramp T, Kil WJ, Halthore A, Gehlhaus K, Shankavaram U, Tofilon PJ, Caplen NJ, Camphausen K.
Inhibition of polo-like kinase 1 in glioblastoma multiforme induces mitotic catastrophe and enhances radiosensitisation.
Eur. J. Cancer. 2013.
[Journal]
2)  Camps J, Pitt JJ, Emons G, Hummon AB, Case CM, Grade M, Jones TL, Nguyen QT, Ghadimi BM, Beissbarth T, Difilippantonio MJ, Caplen NJ, Ried T.
Genetic amplification of the NOTCH modulator LNX2 upregulates the WNT/ß-catenin pathway in colorectal cancer.
Cancer Res. 73: 2003-13, 2013.
[Journal]
3)  Bennett CN, Tomlinson CC, Michalowski AM, Chu IM, Luger D, Mittereder LR, Aprelikova O, Shou J, Piwinica-Worms H, Caplen NJ, Hollingshead MG, Green JE.
Cross-species genomic and functional analyses identify a combination therapy using a CHK1 inhibitor and a ribonucleotide reductase inhibitor to treat triple-negative breast cancer.
Breast Cancer Res. 14: R109, 2012.
[Journal]
4)  Barsotti AM, Beckerman R, Laptenko O, Huppi K, Caplen NJ, Prives C.
p53-Dependent induction of PVT1 and miR-1204.
J. Biol. Chem. 287: 2509-19, 2012.
[Journal]
5)  Hummon AB, Pitt JJ, Camps J, Emons G, Skube SB, Huppi K, Jones TL, Beissbarth T, Kramer F, Grade M, Difilippantonio MJ, Ried T, Caplen NJ.
Systems-wide RNAi analysis of CASP8AP2/FLASH shows transcriptional deregulation of the replication-dependent histone genes and extensive effects on the transcriptome of colorectal cancer cells.
Mol. Cancer. 11: 1, 2012.
[Journal]
6)  Huppi K, Pitt JJ, Wahlberg BM, Caplen NJ.
The 8q24 gene desert: an oasis of non-coding transcriptional activity.
Front Genet. 3: 69, 2012.
[Journal]
7)  Huppi K, Pitt J, Wahlberg B, Caplen NJ.
Genomic instability and mouse microRNAs.
Toxicol. Mech. Methods. 21: 325-33, 2011.
[Journal]
8)  Mackiewicz M, Huppi K, Pitt JJ, Dorsey TH, Ambs S, Caplen NJ.
Identification of the receptor tyrosine kinase AXL in breast cancer as a target for the human miR-34a microRNA.
Breast Cancer Res Treat. 130: 663-79, 2011.
[Journal]
9)  Martin SE, Wu Z, Gehlhaus K, Jones TL, Zhang Y, Guha R, Miyamoto S, Pommier Y, Caplen NJ.
RNAi Screening Identifies TAK1 as a Potential Target for the Enhanced Efficacy of Topoisomerase Inhibitors.
Curr Cancer Drug Targets. 11: 976-86, 2011.
[Journal]
10)  Grade M, Hummon AB, Camps J, Emons G, Spitzner M, Gaedcke J, Hoermann P, Ebner R, Becker H, Difilippantonio MJ, Ghadimi BM, Beissbarth T, Caplen NJ, Ried T.
A genomic strategy for the functional validation of colorectal cancer genes identifies potential therapeutic targets.
Int J Cancer. 128: 1069-79, 2010.
[Journal]
11)  Murrow LM, Garimella SV, Jones TL, Caplen NJ, Lipkowitz S.
Identification of WEE1 as a potential molecular target in cancer cells by RNAi screening of the human tyrosine kinome.
Breast Cancer Res. Treat. 122: 347-57, 2010.
[Journal]
12)  Jobson AG, Lountos GT, Lorenzi PL, Llamas J, Connelly J, Cerna D, Tropea JE, Onda A, Zoppoli G, Kondapaka S, Zhang G, Caplen NJ, Cardellina JH, Yoo SS, Monks A, Self C, Waugh DS, Shoemaker RH, Pommier Y.
Cellular inhibition of checkpoint kinase 2 (Chk2) and potentiation of camptothecins and radiation by the novel Chk2 inhibitor PV1019 [7-nitro-1H-indole-2-carboxylic acid {4-[1-(guanidinohydrazone)-ethyl]-phenyl}-amide].
J. Pharmacol. Exp. Ther. 331: 816-26, 2009.
[Journal]
13)  Zhang YW, Jones TL, Martin SE, Caplen NJ, Pommier Y.
Implication of Checkpoint Kinase-dependent Up-regulation of Ribonucleotide Reductase R2 in DNA Damage Response.
J. Biol. Chem. 284: 18085-95, 2009.
[Journal]
14)  Klootwijk RD, Savelkoul PJ, Ciccone C, Manoli I, Caplen NJ, Krasnewich DM, Gahl WA, Huizing M.
Allele-specific silencing of the dominant disease allele in sialuria by RNA interference.
FASEB J. 22: 3846-52, 2008.
[Journal]
15)  Beck-Engeser GB, Lum AM, Huppi K, Caplen NJ, Wang BB, Wabl M.
Pvt1-encoded microRNAs in oncogenesis.
Retrovirology. 5: 4, 2008.
[Journal]
16)  Calcagno AM, Fostel JM, To KK, Salcido CD, Martin SE, Chewning KJ, Wu CP, Varticovski L, Bates SE, Caplen NJ, Ambudkar SV.
Single-step doxorubicin-selected cancer cells overexpress the ABCG2 drug transporter through epigenetic changes.
Br. J. Cancer. 98: 1515-24, 2008.
[Journal]
17)  Ryan MC, Zeeberg BR, Caplen NJ, Cleland JA, Kahn AB, Liu H, Weinstein JN.
SpliceCenter: a suite of web-based bioinformatic applications for evaluating the impact of alternative splicing on RT-PCR, RNAi, microarray, and peptide-based studies.
BMC Bioinformatics. 9: 313, 2008.
[Journal]
18)  Huppi K, Volfovsky N, Runfola T, Jones TL, Mackiewicz M, Martin SE, Mushinski JF, Stephens R, Caplen NJ.
The identification of microRNAs in a genomically unstable region of human chromosome 8q24.
Mol. Cancer Res. 6: 212-21, 2008.
[Journal]
19)  Martin SE, Caplen NJ.
Applications of RNA Interference in Mammalian Systems.
Annu Rev Genomics Hum Genet. 8: 81-108, 2007.
[Journal]
20)  Huppi K, Volfovsky N, Mackiewicz M, Runfola T, Jones TL, Martin SE, Stephens R, Caplen NJ.
MicroRNAs and genomic instability.
Semin. Cancer Biol. 17: 65-73, 2007.
[Journal]
21)  Martin SE, Jones TL, Thomas CL, Lorenzi PL, Nguyen DA, Runfola T, Gunsior M, Weinstein JN, Goldsmith PK, Lader E, Huppi K, Caplen NJ.
Multiplexing siRNAs to compress RNAi-based screen size in human cells.
Nucleic Acids Res. 35: e57, 2007.
[Journal]
22)  Lorenzi PL, Reinhold WC, Rudelius M, Gunsior M, Shankavaram U, Bussey KJ, Scherf U, Eichler GS, Martin SE, Chin K, Gray JW, Kohn EC, Horak ID, Von Hoff DD, Raffeld M, Goldsmith PK, Caplen NJ, Weinstein JN.
Asparagine synthetase as a causal, predictive biomarker for L-asparaginase activity in ovarian cancer cells.
Mol. Cancer Ther. 5: 2613-23, 2006.
[Journal]
23)  Griesenbach U, Kitson C, Escudero Garcia S, Farley R, Singh C, Somerton L, Painter H, Smith RL, Gill DR, Hyde SC, Chow YH, Hu J, Gray M, Edbrooke M, Ogilvie V, MacGregor G, Scheule RK, Cheng SH, Caplen NJ, Alton EW.
Inefficient cationic lipid-mediated siRNA and antisense oligonucleotide transfer to airway epithelial cells in vivo.
Respir. Res. 7: 26, 2006.
[Journal]
24)  Martin SE, Caplen NJ.
Mismatched siRNAs downregulate mRNAs as a function of target site location.
FEBS Lett. 580: 3694-8, 2006.
[Journal]
25)  Ludwig JA, Szakács G, Martin SE, Chu BF, Cardarelli C, Sauna ZE, Caplen NJ, Fales HM, Ambudkar SV, Weinstein JN, Gottesman MM.
Selective toxicity of NSC73306 in MDR1-positive cells as a new strategy to circumvent multidrug resistance in cancer.
Cancer Res. 66: 4808-15, 2006.
[Journal]
26)  Yanaihara N, Caplen N, Bowman E, Seike M, Kumamoto K, Yi M, Stephens RM, Okamoto A, Yokota J, Tanaka T, Calin GA, Liu CG, Croce CM, Harris CC.
Unique microRNA molecular profiles in lung cancer diagnosis and prognosis.
Cancer Cell. 9: 189-98, 2006.
[Journal]
27)  Huppi K, Martin SE, Caplen NJ.
Defining and Assaying RNAi in mammalian cells.
Molecular Cell. 17: 1-10, 2005.
[Journal]
28)  Kimchi-Sarfaty C, Brittain S, Garfield S, Caplen NJ, Tang Q, Gottesman MM.
Efficient delivery of RNA interference effectors via in vitro-packaged SV40 pseudovirions.
Hum. Gene Ther. 16: 1110-5, 2005.
[Journal]
29)  Dombroski D, Houghtling RA, Labno CM, Precht P, Takesono A, Caplen NJ, Billadeau DD, Wange RL, Burkhardt JK, Schwartzberg PL.
siRNA reveals kinase-independent functions for Itk in TCR-induced regulation of Vav and the actin cytoskeleton.
J. Immunology. 174: 1385-92, 2005.
[Journal]
30)  Caplen NJ.
Gene therapy progress and prospects. Downregulating gene expression: the impact of RNA interference.
Gene Ther. 11: 1241-8, 2004.
[Journal]
31)  Okada T, Caplen NJ, Ramsey WJ, Onodera M, Shimazaki K, Nomoto T, Ajalli R, Wildner O, Morris J, Kume A, Hamada H, Blaese RM, Ozawa K.
In situ generation of pseudotyped retroviral progeny by adenovirus-mediated transduction of tumor cells enhances the killing effect of HSV-tk suicide gene therapy in vitro and in vivo.
J Gene Med. 6: 288-99, 2004.
[Journal]
32)  Caplen NJ.
RNAi quashes polyQ.
Nat Med. 10: 775-6, 2004.
[Journal]
33)  Scacheri PC, Rozenblatt-Rosen O, Caplen NJ, Wolfsberg TG, Umayam L, Lee JC, Hughes CM, Shanmugam KS, Bhattacharjee A, Meyerson M, Collins FS.
Short interfering RNAs can induce unexpected and divergent changes in the levels of untargeted proteins in mammalian cells.
Proc Natl Acad Sci U S A. 101: 1892-7, 2004.
[Journal]
34)  Caplen NJ.
RNAi as a gene therapy approach.
Expert Opin Biol Ther. 3: 575-86, 2003.
[Journal]
35)  Mousses S, Caplen NJ, Cornelison R, Weaver D, Basik M, Hautaniemi S, Elkahloun AG, Lotufo RA, Choudary A, Dougherty ER, Suh E, Kallioniemi O.
RNAi microarray analysis in cultured mammalian cells.
Genome Res. 13: 2341-7, 2003.
[Journal]
36)  Caplen NJ, Mousses S.
Short interfering RNA (siRNA)-mediated RNA interference (RNAi) in human cells.
Ann N Y Acad Sci. 1002: 56-62, 2003.
[Journal]
37)  Caplen NJ.
A new approach to the inhibition of gene expression.
Trends Biotechnol. 20: 49-51, 2002.
[Journal]
38)  Caplen NJ, Zheng Z, Falgout B, Morgan RA.
Inhibition of viral gene expression and replication in mosquito cells by dsRNA-triggered RNA interference.
Mol. Ther. 6: 243-51, 2002.
[Journal]
39)  Caplen NJ, Taylor JP, Statham VS, Tanaka F, Fire A, Morgan RA.
Rescue of polyglutamine-mediated cytotoxicity by double-stranded RNA-mediated RNA interference.
Hum. Mol. Genet. 11: 175-84, 2002.
[Journal]
40)  Caplen NJ.
Cystic fibrosis gene therapy trials and tribulations.
Trends Mol Med. 7: 488, 2001.
[Journal]
41)  Caplen NJ, Parrish S, Imani F, Fire A, Morgan RA.
Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems.
Proc. Natl. Acad. Sci. U.S.A. 98: 9742-7, 2001.
[Journal]
42)  Caplen NJ.
A new gene transfer vector for the lung.
Mol. Med. Today. 6: 460, 2000.
[Journal]
43)  Caplen NJ, Fleenor J, Fire A, Morgan RA.
dsRNA-mediated gene silencing in cultured Drosophila cells: a tissue culture model for the analysis of RNA interference.
Gene. 252: 95-105, 2000.
[Journal]
44)  Caplen NJ.
Is successful gene therapy for retinal disease in sight?.
Mol Med Today. 6: 340, 2000.
[Journal]
45)  Caplen NJ.
Nucleic acid transfer using cationic lipids.
Methods Mol. Biol. 133: 1-19, 2000.
[Journal]
46)  Caplen NJ, Higginbotham JN, Scheel JR, Vahanian N, Yoshida Y, Hamada H, Blaese RM, Ramsey WJ.
Adeno-retroviral chimeric viruses as in vivo transducing agents.
Gene Ther. 6: 454-9, 1999.
[Journal]
47)  Ramsey WJ, Caplen NJ, Li Q, Higginbotham JN, Shah M, Blaese RM.
Adenovirus vectors as transcomplementing templates for the production of replication defective retroviral vectors.
Biochem. Biophys. Res. Commun. 246: 912-9, 1998.
[Journal]
48)  Caplen NJ.
Gene therapy: different strategies for different applications. American Society of Gene Therapy: First Annual Meeting, Seattle, Washington, USA, 28-31 May 1998.
Mol Med Today. 4: 374-5, 1998.
[Journal]
49)  Stern M, Caplen NJ, Browning JE, Griesenbach U, Sorgi F, Huang L, Gruenert DC, Marriot C, Crystal RG, Geddes DM, Alton EW.
The effect of mucolytic agents on gene transfer across a CF sputum barrier in vitro.
Gene Ther. 5: 91-8, 1998.
[Journal]
50)  Stern M, Sorgi F, Hughes C, Caplen NJ, Browning JE, Middleton PG, Gruenert DC, Farr SJ, Huang L, Geddes DM, Alton EW.
The effects of jet nebulisation on cationic liposome-mediated gene transfer in vitro.
Gene Ther. 5: 583-93, 1998.
[Journal]
51)  Davies JC, Stern M, Dewar A, Caplen NJ, Munkonge FM, Pitt T, Sorgi F, Huang L, Bush A, Geddes DM, Alton EW.
CFTR gene transfer reduces the binding of Pseudomonas aeruginosa to cystic fibrosis respiratory epithelium.
Am. J. Respir. Cell Mol. Biol. 16: 657-63, 1997.
[Journal]
52)  Li D, Wang D, Majumdar S, Jany B, Durham SR, Cottrell J, Caplen N, Geddes DM, Alton EW, Jeffery PK.
Localization and up-regulation of mucin (MUC2) gene expression in human nasal biopsies of patients with cystic fibrosis.
J. Pathol. 181: 305-10, 1997.
[Journal]
53)  Smith SN, Steel DM, Middleton PG, Munkonge FM, Geddes DM, Caplen NJ, Porteous DJ, Dorin JR, Alton EW.
Bioelectric characteristics of exon 10 insertional cystic fibrosis mouse: comparison with humans.
Am. J. Physiol. 268: C297-307, 1995.
[Journal]
54)  Caplen NJ, Kinrade E, Sorgi F, Gao X, Gruenert D, Geddes D, Coutelle C, Huang L, Alton EW, Williamson R.
In vitro liposome-mediated DNA transfection of epithelial cell lines using the cationic liposome DC-Chol/DOPE.
Gene Ther. 2: 603-13, 1995.
[Journal]
55)  Caplen NJ, Alton EW, Middleton PG, Dorin JR, Stevenson BJ, Gao X, Durham SR, Jeffery PK, Hodson ME, Coutelle C.
Liposome-mediated CFTR gene transfer to the nasal epithelium of patients with cystic fibrosis.
Nat. Med. 1: 39-46, 1995.
[Journal]
56)  Stern M, Munkonge FM, Caplen NJ, Sorgi F, Huang L, Geddes DM, Alton EW.
Quantitative fluorescence measurements of chloride secretion in native airway epithelium from CF and non-CF subjects.
Gene Ther. 2: 766-74, 1995.
[Journal]
57)  Caplen NJ, Gao X, Hayes P, Elaswarapu R, Fisher G, Kinrade E, Chakera A, Schorr J, Hughes B, Dorin JR.
Gene therapy for cystic fibrosis in humans by liposome-mediated DNA transfer: the production of resources and the regulatory process.
Gene Ther. 1: 139-47, 1994.
[Journal]
58)  Middleton PG, Caplen NJ, Gao X, Huang L, Gaya H, Geddes DM, Alton EW.
Nasal application of the cationic liposome DC-Chol:DOPE does not alter ion transport, lung function or bacterial growth.
Eur. Respir. J. 7: 442-5, 1994.
[Journal]
59)  Coutelle C, Caplen N, Hart S, Huxley C, Williamson R.
Gene therapy for cystic fibrosis.
Arch. Dis. Child. 68: 437-40, 1993.
[Journal]
60)  Alton EW, Middleton PG, Caplen NJ, Smith SN, Steel DM, Munkonge FM, Jeffery PK, Geddes DM, Hart SL, Williamson R.
Non-invasive liposome-mediated gene delivery can correct the ion transport defect in cystic fibrosis mutant mice.
Nat. Genet. 5: 135-42, 1993.
[Journal]
61)  Alton E, Caplen N, Geddes D, Williamson R.
New treatments for cystic fibrosis.
Br. Med. Bull. 48: 785-804, 1992.
[Journal]
62)  Caplen NJ, Patel A, Millward A, Campbell RD, Ratanachaiyavong S, Wong FS, Demaine AG.
Complement C4 and heat shock protein 70 (HSP70) genotypes and type I diabetes mellitus.
Immunogenetics. 32: 427-30, 1990.
[Journal]
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This page was last updated on 7/29/2013.