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Barry R. O'Keefe, Ph.D.

Selected Publications

1)  O'Keefe BR.
Biologically active proteins from natural product extracts.
J Nat Prod. 64: 1373-81, 2001.
2)  Mori T, O'Keefe BR, Sowder RC, Bringans S, Gardella R, Berg S, Cochran P, Turpin JA, Buckheit RW, McMahon JB, Boyd MR.
Isolation and characterization of griffithsin, a novel HIV-inactivating protein, from the red alga Griffithsia sp.
J Biol Chem. 280: 9345-53, 2005.
3)  Sasiela CA, Stewart DH, Kitagaki J, Safiran YJ, Yang Y, Weissman AM, Oberoi P, Davydov IV, Goncharova E, Beutler JA, McMahon JB, O'Keefe BR.
Identification of inhibitors for MDM2 ubiquitin ligase activity from natural product extracts by a novel high-throughput electrochemiluminescent screen.
J Biomol Screen. 13: 229-37, 2008.
4)  O'Keefe BR, Vojdani F, Buffa V, Shattock RJ, Montefiori DC, Bakke J, Mirsalis J, d'Andrea AL, Hume SD, Bratcher B, Saucedo CJ, McMahon JB, Pogue GP, Palmer KE.
Scaleable manufacture of HIV-1 entry inhibitor griffithsin and validation of its safety and efficacy as a topical microbicide component.
Proc. Natl. Acad. Sci. U.S.A. 106: 6099-104, 2009.
5)  O'Keefe BR, Giomarelli B, Barnard DL, Shenoy SR, Chan PK, McMahon JB, Palmer KE, Barnett BW, Meyerholz DK, Wohlford-Lenane CL, McCray PB.
Broad-spectrum in vitro activity and in vivo efficacy of the antiviral protein griffithsin against emerging viruses of the family Coronaviridae.
J. Virol. 84: 2511-21, 2010.
6)  Takebe Y, Saucedo CJ, Lund G, Uenishi R, Hase S, Tsuchiura T, Kneteman N, Ramessar K, Tyrrell DL, Shirakura M, Wakita T, McMahon JB, O'Keefe BR.
Antiviral lectins from red and blue-green algae show potent in vitro and in vivo activity against hepatitis C virus.
PLoS ONE. 8: e64449, 2013.
7)  Nguyen TX, Morrell A, Conda-Sheridan M, Marchand C, Agama K, Bermingham A, Stephen AG, Chergui A, Naumova A, Fisher R, O'Keefe BR, Pommier Y, Cushman M.
Correction to Synthesis and Biological Evaluation of the First Dual Tyrosyl-DNA Phosphodiesterase I (Tdp1)-Topoisomerase I (Top1) Inhibitors.
J. Med. Chem. 55: 7297, 2012.
8)  Nguyen TX, Morrell A, Conda-Sheridan M, Marchand C, Agama K, Bermingam A, Stephen AG, Chergui A, Naumova A, Fisher R, O'Keefe BR, Pommier Y, Cushman M.
Synthesis and biological evaluation of the first dual tyrosyl-DNA phosphodiesterase I (Tdp1)-topoisomerase I (Top1) inhibitors.
J. Med. Chem. 55: 4457-78, 2012.
9)  Alexandre KB, Gray ES, Mufhandu H, McMahon JB, Chakauya E, O'Keefe BR, Chikwamba R, Morris L.
The lectins griffithsin, cyanovirin-N and scytovirin inhibit HIV-1 binding to the DC-SIGN receptor and transfer to CD4(+) cells.
Virology. 423: 175-86, 2012.
10)  Alexandre KB, Gray ES, Pantophlet R, Moore PL, McMahon JB, Chakauya E, O'Keefe BR, Chikwamba R, Morris L.
Binding of the mannose-specific lectin, griffithsin, to HIV-1 gp120 exposes the CD4-binding site.
J. Virol. 85: 9039-50, 2011.
11)  Kouokam JC, Huskens D, Schols D, Johannemann A, Riedell SK, Walter W, Walker JM, Matoba N, O'Keefe BR, Palmer KE.
Investigation of griffithsin"s interactions with human cells confirms its outstanding safety and efficacy profile as a microbicide candidate.
PLoS ONE. 6: e22635, 2011.
12)  Alexandre KB, Gray ES, Lambson BE, Moore PL, Choge IA, Mlisana K, Karim SS, McMahon J, O'Keefe B, Chikwamba R, Morris L.
Mannose-rich glycosylation patterns on HIV-1 subtype C gp120 and sensitivity to the lectins, Griffithsin, Cyanovirin-N and Scytovirin.
Virology. 402: 187-96, 2010.
13)  Moulaei T, Shenoy SR, Giomarelli B, Thomas C, McMahon JB, Dauter Z, O'Keefe BR, Wlodawer A.
Monomerization of viral entry inhibitor griffithsin elucidates the relationship between multivalent binding to carbohydrates and anti-HIV activity.
Structure. 18: 1104-15, 2010.
14)  Chung S, Wendeler M, Rausch JW, Beilhartz G, Gotte M, O'Keefe BR, Bermingham A, Beutler JA, Liu S, Zhuang X, Le Grice SF.
Structure-activity analysis of vinylogous urea inhibitors of human immunodeficiency virus-encoded ribonuclease H.
Antimicrob. Agents Chemother. 54: 3913-21, 2010.
15)  Bushkin GG, Ratner DM, Cui J, Banerjee S, Duraisingh MT, Jennings CV, Dvorin JD, Gubbels M, Robertson SD, Steffen M, O'Keefe BR, Robbins PW, Samuelson J.
Suggestive evidence for Darwinian Selection against asparagine-linked glycans of Plasmodium falciparum and Toxoplasma gondii.
Eukaryot Cell. 9: 228-41, 2010.
16)  Carpentieri A, Ratner DM, Ghosh SK, Banerjee S, Bushkin GG, Cui J, Lubrano M, Steffen M, Costello CE, O'Keefe B, Robbins PW, Samuelson J.
The antiretroviral lectin cyanovirin-N targets well-known and novel targets on the surface of Entamoeba histolytica trophozoites.
Eukaryotic Cell. 9: 1661-8, 2010.
17)  Moulaei T, Stuchlik O, Reed M, Yuan W, Pohl J, Lu W, Haugh-Krumpe L, O'Keefe BR, Wlodawer A.
Topology of the disulfide bonds in the antiviral lectin scytovirin.
Protein Sci. 19(9): 1649-61, 2010.
18)  Clement JA, Kitagaki J, Yang Y, Saucedo CJ, O'Keefe BR, Weissman AM, McKee TC, McMahon JB.
Discovery of new pyridoacridine alkaloids from Lissoclinum cf. badium that inhibit the ubiquitin ligase activity of Hdm2 and stabilize p53.
Bioorg. Med. Chem. 16: 10022-8, 2008.
19)  Weissman AM, Yang Y, Kitagaki J, Sasiela CA, Beutler JA, O'Keefe BR.
Inhibiting Hdm2 and ubiquitin-activating enzyme: targeting the ubiquitin conjugating system in cancer.
Ernst Schering Found Symp Proc. 171-90, 2008.
20)  Smee DF, Bailey KW, Wong MH, O'Keefe BR, Gustafson KR, Mishin VP, Gubareva LV.
Treatment of influenza A (H1N1) virus infections in mice and ferrets with cyanovirin-N.
Antiviral Res. 80: 266-71, 2008.
21)  Wendeler M, Lee HF, Bermingham A, Miller JT, Chertov O, Bona MK, Baichoo NS, Ehteshami M, Beutler J, O'Keefe BR, Götte M, Kvaratskhelia M, Le Grice S.
Vinylogous ureas as a novel class of inhibitors of reverse transcriptase-associated ribonuclease H activity.
ACS Chem. Biol. 3: 635-44, 2008.
22)  Takada K, Bermingham A, O'Keefe BR, Wamiru A, Beutler JA, Le Grice SF, Lloyd J, Gustafson KR, McMahon JB.
An HIV RNase H inhibitory 1,3,4,5-tetragalloylapiitol from the African plant Hylodendron gabunensis.
J. Nat. Prod. 70: 1647-9, 2007.
23)  Moulaei T, Botos I, Ziolkowska NE, Bokesch HR, Krumpe LR, McKee TC, O'Keefe BR, Dauter Z, Wlodawer A.
Atomic-resolution crystal structure of the antiviral lectin scytovirin.
Protein Sci. 16: 2756-60, 2007.
24)  Ziolkowska NE, Shenoy SR, O'Keefe BR, Wlodawer A.
Crystallographic studies of the complexes of antiviral protein griffithsin with glucose and N-acetylglucosamine.
Protein Sci. 16: 1485-9, 2007.
25)  Ziolkowska NE, Shenoy SR, O'Keefe BR, McMahon JB, Palmer KE, Dwek RA, Wormald MR, Wlodawer A.
Crystallographic, thermodynamic, and molecular modeling studies of the mode of binding of oligosaccharides to the potent antiviral protein griffithsin.
Proteins. 67: 661-70, 2007.
26)  Emau P, Tian B, O'keefe BR, Mori T, McMahon JB, Palmer KE, Jiang Y, Bekele G, Tsai CC.
Griffithsin, a potent HIV entry inhibitor, is an excellent candidate for anti-HIV microbicide.
J. Med. Primatol. 36: 244-53, 2007.
27)  Smee DF, Wandersee MK, Checketts MB, O'Keefe BR, Saucedo C, Boyd MR, Mishin VP, Gubareva LV.
Influenza A (H1N1) virus resistance to cyanovirin-N arises naturally during adaptation to mice and by passage in cell culture in the presence of the inhibitor.
Antivir. Chem. Chemother. 18: 317-27, 2007.
28)  McFeeters RL, Xiong C, O'Keefe BR, Bokesch HR, McMahon JB, Ratner DM, Castelli R, Seeberger PH, Byrd RA.
The novel fold of scytovirin reveals a new twist for antiviral entry inhibitors.
J. Mol. Biol. 369: 451-61, 2007.
29)  Ziolkowska NE, O'Keefe BR, Mori T, Zhu C, Giomarelli B, Vojdani F, Palmer KE, McMahon JB, Wlodawer A.
Domain-swapped structure of the potent antiviral protein griffithsin and its mode of carbohydrate binding.
Structure. 14: 1127-35, 2006.
30)  Xiong C, O'Keefe BR, Botos I, Wlodawer A, McMahon JB.
Overexpression and purification of scytovirin, a potent, novel anti-HIV protein from the cultured cyanobacterium Scytonema varium.
Protein Expr. Purif. 46: 233-9, 2006.
31)  Xiong C, O'Keefe BR, Byrd RA, McMahon JB.
Potent anti-HIV activity of scytovirin domain 1 peptide.
Peptides. 27: 1668-75, 2006.
32)  Maderia M, Wu J, Bax A, Shenoy S, O'Keefe B, Marquez VE, Barchi JJ.
Engineering DNA topology with locked nucleosides: a structural study.
Nucleosides Nucleotides Nucleic Acids. 24: 687-90, 2005.
33)  Witvrouw M, Fikkert V, Hantson A, Pannecouque C, O'keefe BR, McMahon J, Stamatatos L, de Clercq E, Bolmstedt A.
Resistance of human immunodeficiency virus type 1 to the high-mannose binding agents cyanovirin N and concanavalin A.
J. Virol. 79: 7777-84, 2005.
34)  Milanowski DJ, Rashid MA, Gustafson KR, O'Keefe BR, Nawrocki JP, Pannell LK, Boyd MR.
Cyclonellin, a new cyclic octapeptide from the marine sponge Axinella carteri.
J. Nat. Prod. 67: 441-4, 2004.
35)  Bringans SD, O'Keefe BR, Bray M, Whitehouse CA, Boyd MR.
Development of a fluorescent microplate assay for determining cyanovirin-N levels in plasma.
Anal Bioanal Chem. 380: 269-74, 2004.
36)  Bokesch HR, Charan RD, Meragelman KM, Beutler JA, Gardella R, O'Keefe BR, McKee TC, McMahon JB.
Isolation and characterization of anti-HIV peptides from Dorstenia contrajerva and Treculia obovoidea.
FEBS Lett. 567: 287-90, 2004.
37)  Adams EW, Ratner DM, Bokesch HR, McMahon JB, O'Keefe BR, Seeberger PH.
Oligosaccharide and glycoprotein microarrays as tools in HIV glycobiology; glycan-dependent gp120/protein interactions.
Chem. Biol. 11: 875-81, 2004.
38)  Ratner DM, Adams EW, Su J, O'Keefe BR, Mrksich M, Seeberger PH.
Probing protein-carbohydrate interactions with microarrays of synthetic oligosaccharides.
Chembiochem. 5: 379-82, 2004.
39)  Bokesch HR, O'Keefe BR, McKee TC, Pannell LK, Patterson GM, Gardella RS, Sowder RC, Turpin J, Watson K, Buckheit RW, Boyd MR.
A potent novel anti-HIV protein from the cultured cyanobacterium Scytonema varium.
Biochemistry. 42: 2578-84, 2003.
40)  Barrientos LG, O'Keefe BR, Bray M, Sanchez A, Gronenborn AM, Boyd MR.
Cyanovirin-N binds to the viral surface glycoprotein, GP1,2 and inhibits infectivity of Ebola virus.
Antiviral Res. 58: 47-56, 2003.
41)  Adams EW, Ueberfeld J, Ratner DM, O'Keefe BR, Walt DR, Seeberger PH.
Encoded fiber-optic microsphere arrays for probing protein-carbohydrate interactions.
Angew. Chem. Int. Ed. Engl. 42: 5317-20, 2003.
42)  O'Keefe BR, Smee DF, Turpin JA, Saucedo CJ, Gustafson KR, Mori T, Blakeslee D, Buckheit R, Boyd MR.
Potent anti-influenza activity of cyanovirin-N and interactions with viral hemagglutinin.
Antimicrob Agents Chemother. 47: 2518-25, 2003.
43)  Barrientos LG, Louis JM, Hung J, Smith TH, O'Keefe BR, Gardella RS, Mori T, Boyd MR, Gronenborn AM.
Design and initial characterization of a circular permuted variant of the potent HIV-inactivating protein cyanovirin-N.
Proteins. 46: 153-60, 2002.
44)  Beutler JA, McMahon JB, Johnson TR, O'Keefe BR, Buzzell RA, Robbins D, Gardella R, Wilson J, Boyd MR.
High throughput screening for cyanovirin-N mimetics binding to HIV-1 gp41.
J Biomol Screen. 7: 105-10, 2002.
45)  Shenoy SR, Barrientos LG, Ratner DM, O'Keefe BR, Seeberger PH, Gronenborn AM, Boyd MR.
Multisite and multivalent binding between cyanovirin-N and branched oligomannosides: calorimetric and NMR characterization.
Chem. Biol. 9: 1109-18, 2002.
46)  Botos I, O'Keefe BR, Shenoy SR, Cartner LK, Ratner DM, Seeberger PH, Boyd MR, Wlodawer A.
Structures of the complexes of a potent anti-HIV protein cyanovirin-N and high mannose oligosaccharides.
J. Biol. Chem. 277: 34336-42, 2002.
47)  Barrientos LG, Louis JM, Botos I, Mori T, Han Z, O'Keefe BR, Boyd MR, Wlodawer A, Gronenborn AM.
The domain-swapped dimer of cyanovirin-N is in a metastable folded state: reconciliation of X-ray and NMR structures.
Structure. 10: 673-86, 2002.
48)  Bolmstedt AJ, O'Keefe BR, Shenoy SR, McMahon JB, Boyd MR.
Cyanovirin-N defines a new class of antiviral agent targeting N-linked, high-mannose glycans in an oligosaccharide-specific manner.
Mol. Pharmacol. 59: 949-54, 2001.
49)  Shenoy SR, O'Keefe BR, Bolmstedt AJ, Cartner LK, Boyd MR.
Selective interactions of the human immunodeficiency virus-inactivating protein cyanovirin-N with high-mannose oligosaccharides on gp120 and other glycoproteins.
J. Pharmacol. Exp. Ther. 297: 704-10, 2001.
50)  O'Keefe BR, Shenoy SR, Xie D, Zhang W, Muschik JM, Currens MJ, Chaiken I, Boyd MR.
Analysis of the interaction between the HIV-inactivating protein cyanovirin-N and soluble forms of the envelope glycoproteins gp120 and gp41.
Mol. Pharmacol. 58: 982-92, 2000.
51)  McMahon JB, Beutler JA, O'Keefe BR, Goodrum CB, Myers MA, Boyd MR.
Development of a cyanovirin-N-HIV-1 gp120 binding assay for high throughput screening of natural product extracts by time-resolved fluorescence.
J Biomol Screen. 5: 169-76, 2000.
52)  Charan RD, Munro MH, O'Keefe BR, McKee TC, Currens MJ, Pannell LK, Boyd MR.
Isolation and characterization of Myrianthus holstii lectin, a potent HIV-1 inhibitory protein from the plant Myrianthus holstii(1).
J. Nat. Prod. 63: 1170-4, 2000.
53)  O'Keefe BR, Erim T, Beutler JA, Cardellina JH, Gulakowski RJ, Krepps BL, McMahon JB, Sowder RC, Johnson DG, Buckheit RW, Halliday S, Boyd MR.
Isolation and characterization of adociavirin, a novel HIV-inhibitory protein from the sponge Adocia sp.
FEBS Lett. 431: 85-90, 1998.
54)  Mariner JM, McMahon JB, O'Keefe BR, Nagashima K, Boyd MR.
The HIV-inactivating protein, cyanovirin-N, does not block gp120-mediated virus-to-cell binding.
Biochem. Biophys. Res. Commun. 248: 841-5, 1998.
55)  Boyd MR, Gustafson KR, McMahon JB, Shoemaker RH, O'Keefe BR, Mori T, Gulakowski RJ, Wu L, Rivera MI, Laurencot CM, Currens MJ, Cardellina JH, Buckheit RW, Nara PL, Pannell LK, Sowder RC, Henderson LE.
Discovery of cyanovirin-N, a novel human immunodeficiency virus-inactivating protein that binds viral surface envelope glycoprotein gp120: potential applications to microbicide development.
Antimicrob. Agents Chemother. 41: 1521-30, 1997.
56)  O'Keefe BR, Beutler JA, Cardellina JH, Gulakowski RJ, Krepps BL, McMahon JB, Sowder RC, Henderson LE, Pannell LK, Pomponi SA, Boyd MR.
Isolation and characterization of niphatevirin, a human-immunodeficiency-virus-inhibitory glycoprotein from the marine sponge Niphates erecta.
Eur. J. Biochem. 245: 47-53, 1997.
57)  O'Keefe BR, Beutler JA, Cardellina JH, Prather TR, Shoemaker RH, Sowder RC, Henderson LE, Pannell LK, Boyd MR.
Isolation of a novel Kunitz family protease inhibitor in association with Tethya hemolysin from the sponge Tethya ingalli.
J. Nat. Prod. 60: 1094-9, 1997.
58)  O'Keefe BR, Beecher C.
Isolation and Characterization of S-Adenosyl-L-Methionine:Tetrahydroberberine-cis-N-Methyltransferase from Suspension Cultures of Sanguinaria canadensis L.
Plant Physiol. 105: 395-403, 1994.
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This page was last updated on 7/13/2014.