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Our Science – Kruhlak Website

Michael J. Kruhlak, Ph.D.

Selected Publications

1)  Nguyen VP, Chen J, Petrus MN, Goldman CK, Kruhlak MJ, Bamford RN, Waldmann TA.
A new domain in the Toll/IL-1R domain-containing adaptor inducing interferon-ß factor protein amino terminus is important for tumor necrosis factor-a receptor-associated factor 3 association, protein stabilization and interferon signaling.
J Innate Immun. 6: 377-93, 2014.
2)  Wang P, Zhou Z, Hu A, Ponte de Albuquerque C, Zhou Y, Hong L, Sierecki E, Ajiro M, Kruhlak M, Harris C, Guan KL, Zheng ZM, Newton AC, Sun P, Zhou H, Fu XD.
Both decreased and increased SRPK1 levels promote cancer by interfering with PHLPP-mediated dephosphorylation of Akt.
Mol. Cell. 54: 378-91, 2014.
3)  Polato F, Callen E, Wong N, Faryabi R, Bunting S, Chen HT, Kozak M, Kruhlak MJ, Reczek CR, Lee WH, Ludwig T, Baer R, Feigenbaum L, Jackson S, Nussenzweig A.
CtIP-mediated resection is essential for viability and can operate independently of BRCA1.
J. Exp. Med. 211: 1027-36, 2014.
4)  Yang Y, Schmitz R, Mitala J, Whiting A, Xiao W, Ceribelli M, Wright GW, Zhao H, Yang Y, Xu W, Rosenwald A, Ott G, Gascoyne RD, Connors JM, Rimsza LM, Campo E, Jaffe ES, Delabie J, Smeland EB, Braziel RM, Tubbs RR, Cook JR, Weisenburger DD, Chan WC, Wiestner A, Kruhlak MJ, Iwai K, Bernal F, Staudt LM.
Essential role of the linear ubiquitin chain assembly complex in lymphoma revealed by rare germline polymorphisms.
Cancer Discov. 4: 480-93, 2014.
5)  Williams JA, Zhang J, Jeon H, Nitta T, Ohigashi I, Klug D, Kruhlak MJ, Choudhury B, Sharrow SO, Granger L, Adams A, Eckhaus MA, Jenkinson SR, Richie ER, Gress RE, Takahama Y, Hodes RJ.
Thymic medullary epithelium and thymocyte self-tolerance require cooperation between CD28-CD80/86 and CD40-CD40L costimulatory pathways.
J. Immunol. 192: 630-40, 2014.
6)  Callen E, Di Virgilio M, Kruhlak MJ, Nieto-Soler M, Wong N, Chen HT, Faryabi RB, Polato F, Santos M, Starnes LM, Wesemann DR, Lee JE, Tubbs A, Sleckman BP, Daniel JA, Ge K, Alt FW, Fernandez-Capetillo O, Nussenzweig MC, Nussenzweig A.
53BP1 Mediates Productive and Mutagenic DNA Repair through Distinct Phosphoprotein Interactions.
Cell. 153: 1266-80, 2013.
7)  Lamy L, Ngo VN, Emre NC, Shaffer AL, Yang Y, Tian E, Nair V, Kruhlak MJ, Zingone A, Landgren O, Staudt LM.
Control of autophagic cell death by caspase-10 in multiple myeloma.
Cancer Cell. 23: 435-49, 2013.
8)  Kruhlak MJ.
Correlative fluorescence and EFTEM imaging of the organized components of the mammalian nucleus.
Methods Mol. Biol. 950: 397-416, 2013.
9)  Massimelli MJ, Majerciak V, Kruhlak M, Zheng ZM.
Interplay between polyadenylate-binding protein 1 and Kaposi"s sarcoma-associated herpesvirus ORF57 in accumulation of polyadenylated nuclear RNA, a viral long noncoding RNA.
J. Virol. 87: 243-56, 2013.
10)  Sousa AA, Kruhlak MJ.
Introduction: nanoimaging techniques in biology.
Methods Mol. Biol. 950: 1-10, 2013.
11)  Jenkinson SR, Williams JA, Jeon H, Zhang J, Nitta T, Ohigashi I, Kruhlak M, Zuklys S, Sharrow S, Adams A, Granger L, Choi Y, Siebenlist U, Bishop GA, Hollander GA, Takahama Y, Hodes RJ.
TRAF3 enforces the requirement for T cell cross-talk in thymic medullary epithelial development.
Proc. Natl. Acad. Sci. U.S.A. 110: 21107-12, 2013.
12)  Ben-Aissa K, Patino-Lopez G, Belkina NV, Maniti O, Rosales T, Hao JJ, Kruhlak MJ, Knutson JR, Picart C, Shaw S.
Activation of Moesin, a Protein That Links Actin Cytoskeleton to the Plasma Membrane, Occurs by Phosphatidylinositol 4,5-bisphosphate (PIP2) Binding Sequentially to Two Sites and Releasing an Autoinhibitory Linker.
J. Biol. Chem. 287: 16311-23, 2012.
13)  Tai X, Van Laethem F, Pobezinsky L, Guinter T, Sharrow SO, Adams A, Granger L, Kruhlak M, Lindsten T, Thompson CB, Feigenbaum L, Singer A.
Basis of CTLA-4 function in regulatory and conventional CD4+ T cells.
Blood. 119: 5155-63, 2012.
14)  Liu Y, Belkina NV, Park C, Nambiar R, Loughhead SM, Patino-Lopez G, Ben-Aissa K, Hao JJ, Kruhlak MJ, Qi H, von Andrian UH, Kehrl JH, Tyska MJ, Shaw S.
Constitutively active ezrin increases membrane tension, slows migration, and impedes endothelial transmigration of lymphocytes in vivo in mice.
Blood. 119: 445-53, 2012.
15)  Lonsdorf AS, Krämer BF, Fahrleitner M, Schönberger T, Gnerlich S, Ring S, Gehring S, Schneider SW, Kruhlak MJ, Meuth SG, Nieswandt B, Gawaz M, Enk AH, Langer HF.
Engagement of alphaIIbbeta3 (GPIIb/IIIa) with alphavbeta3 integrin mediates interaction of melanoma cells with platelets: a connection to hematogenous metastasis.
J. Biol. Chem. 287: 2168-78, 2012.
16)  Müller JR, Waldmann TA, Kruhlak MJ, Dubois S.
Paracrine and transpresentation functions of IL-15 are mediated by diverse splice versions of IL-15Ra in human monocytes and dendritic cells.
J. Biol. Chem. 287: 40328-38, 2012.
17)  Langer HF, Choi EY, Zhou H, Schleicher R, Chung KJ, Tang Z, Göbel K, Bdeir K, Chatzigeorgiou A, Wong C, Bhatia S, Kruhlak MJ, Rose JW, Burns JB, Hill KE, Qu H, Zhang Y, Lehrmann E, Becker KG, Wang Y, Simon DI, Nieswandt B, Lambris JD, Li X, Meuth SG, Kubes P, Chavakis T.
Platelets contribute to the pathogenesis of experimental autoimmune encephalomyelitis.
Circ. Res. 110: 1202-10, 2012.
18)  Sousa AA, Morgan JT, Brown PH, Adams A, Mudiyanselage P, Zhang G, Ackerson CJ, Kruhlak MJ, Leapman RD.
Synthesis, Characterization, and Direct Intracellular Imaging of Ultrasmall and Uniform Glutathione-Coated Gold Nanoparticles.
Small. 8: 2277-86, 2012.
19)  Kang JG, Majerciak V, Uldrick TS, Wang X, Kruhlak M, Yarchoan R, Zheng ZM.
Kaposi's sarcoma-associated herpesviral IL-6 and human IL-6 open reading frames contain miRNA binding sites and are subject to cellular miRNA regulation.
J. Pathol. 225: 378-89, 2011.
20)  Majerciak V, Uranishi H, Kruhlak M, Pilkington GR, Massimelli MJ, Bear J, Pavlakis GN, Felber BK, Zheng ZM.
Kaposi's sarcoma-associated herpesvirus ORF57 interacts with cellular RNA export cofactors RBM15 and OTT3 to promote expression of viral ORF59.
J. Virol. 85: 1528-40, 2011.
21)  Kang JG, Pripuzova N, Majerciak V, Kruhlak M, Le SY, Zheng ZM.
Kaposi's sarcoma-associated herpesvirus ORF57 promotes escape of viral and human interleukin-6 from microRNA-mediated suppression.
J. Virol. 85: 2620-30, 2011.
22)  Majerciak V, Kruhlak M, Dagur PK, McCoy JP, Zheng ZM.
Caspase-7 cleavage of Kaposi sarcoma-associated herpesvirus ORF57 confers a cellular function against viral lytic gene expression.
J. Biol. Chem. 285: 11297-307, 2010.
23)  Langer HF, Chung K, Orlova VV, Choi EY, Kaul S, Kruhlak MJ, Alatsatianos M, Deangelis RA, Roche PA, Magotti P, Li X, Economopoulou M, Rafail S, Lambris JD, Chavakis T.
Complement-mediated inhibition of neovascularization reveals a point of convergence between innate immunity and angiogenesis.
Blood. 116: 4395-403, 2010.
24)  Rui L, Emre NC, Kruhlak MJ, Chung HJ, Steidl C, Slack G, Wright GW, Lenz G, Ngo VN, Shaffer AL, Xu W, Zhao H, Yang Y, Lamy L, Davis RE, Xiao W, Powell J, Maloney D, Thomas CJ, Möller P, Rosenwald A, Ott G, Muller-Hermelink HK, Savage K, Connors JM, Rimsza LM, Campo E, Jaffe ES, Delabie J, Smeland EB, Weisenburger DD, Chan WC, Gascoyne RD, Levens D, Staudt LM.
Cooperative epigenetic modulation by cancer amplicon genes.
Cancer Cell. 18: 590-605, 2010.
25)  Nakata H, Kruhlak M, Kamata W, Ogata-Aoki H, Li J, Maeda K, Ghosh AK, Mitsuya H.
Effects of CC chemokine receptor 5 (CCR5) inhibitors on the dynamics of CCR5 and CC-chemokine-CCR5 interactions.
Antivir Ther. 15: 321-31, 2010.
26)  Katragkou A, Kruhlak MJ, Simitsopoulou M, Chatzimoschou A, Taparkou A, Cotten CJ, Paliogianni F, Diza-Mataftsi E, Tsantali C, Walsh TJ, Roilides E.
Interactions between human phagocytes and Candida albicans biofilms alone and in combination with antifungal agents.
J. Infect. Dis. 201: 1941-9, 2010.
27)  Patino-Lopez G, Aravind L, Dong X, Kruhlak MJ, Ostap EM, Shaw S.
Myosin 1G is an abundant class I myosin in lymphocytes whose localization at the plasma membrane depends on its ancient divergent pleckstrin homology (PH) domain (Myo1PH).
J. Biol. Chem. 285: 8675-86, 2010.
28)  Callén E, Bunting S, Huang CY, Difilippantonio MJ, Wong N, Khor B, Mahowald G, Kruhlak MJ, Ried T, Sleckman BP, Nussenzweig A.
Chimeric IgH-TCRalpha/delta translocations in T lymphocytes mediated by RAG.
Cell Cycle. 8: 2408-12, 2009.
29)  Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM.
Control of the papillomavirus early-to-late switch by differentially expressed SRp20.
J. Virol. 83: 167-80, 2009.
30)  Kruhlak MJ, Celeste A, Nussenzweig A.
Monitoring DNA breaks in optically highlighted chromatin in living cells by laser scanning confocal microscopy.
Methods Mol. Biol. 523: 125-40, 2009.
31)  Hao JJ, Liu Y, Kruhlak M, Debell KE, Rellahan BL, Shaw S.
Phospholipase C-mediated hydrolysis of PIP2 releases ERM proteins from lymphocyte membrane.
J. Cell Biol. 184: 451-62, 2009.
32)  Difilippantonio S, Gapud E, Wong N, Huang CY, Mahowald G, Chen HT, Kruhlak MJ, Callen E, Livak F, Nussenzweig MC, Sleckman BP, Nussenzweig A.
53BP1 facilitates long-range DNA end-joining during V(D)J recombination.
Nature. 456: 529-33, 2008.
33)  Majerciak V, Yamanegi K, Allemand E, Kruhlak M, Krainer AR, Zheng ZM.
Kaposi's sarcoma-associated herpesvirus ORF57 functions as a viral splicing factor and promotes expression of intron-containing viral lytic genes in spliceosome-mediated RNA splicing.
J. Virol. 82: 2792-801, 2008.
34)  Patino-Lopez G, Dong X, Ben-Aissa K, Bernot KM, Itoh T, Fukuda M, Kruhlak MJ, Samelson LE, Shaw S.
Rab35 and its GAP EPI64C in T cells regulate receptor recycling and immunological synapse formation.
J. Biol. Chem. 283: 18323-30, 2008.
35)  Difilippantonio S, Celeste A, Kruhlak MJ, Lee Y, Difilippantonio MJ, Feigenbaum L, Jackson SP, McKinnon PJ, Nussenzweig A.
Distinct domains in Nbs1 regulate irradiation-induced checkpoints and apoptosis.
J. Exp. Med. 204: 1003-11, 2007.
36)  Kim JA, Kruhlak M, Dotiwala F, Nussenzweig A, Haber JE.
Heterochromatin is refractory to {gamma}-H2AX modification in yeast and mammals.
J. Cell Biol. 178: 209-18, 2007.
37)  Kwon O, Eck P, Chen S, Corpe CP, Lee JH, Kruhlak M, Levine M.
Inhibition of the intestinal glucose transporter GLUT2 by flavonoids.
FASEB J. 21: 366-77, 2007.
38)  Hope WW, Kruhlak MJ, Lyman CA, Petraitiene R, Petraitis V, Francesconi A, Kasai M, Mickiene D, Sein T, Peter J, Kelaher AM, Hughes JE, Cotton MP, Cotten CJ, Bacher J, Tripathi S, Bermudez L, Maugel TK, Zerfas PM, Wingard JR, Drusano GL, Walsh TJ.
Pathogenesis of Aspergillus fumigatus and the kinetics of galactomannan in an in vitro model of early invasive pulmonary aspergillosis: implications for antifungal therapy.
J. Infect. Dis. 195: 455-66, 2007.
39)  Liu Y, Kruhlak MJ, Hao JJ, Shaw S.
Rapid T cell receptor-mediated SHP-1 S591 phosphorylation regulates SHP-1 cellular localization and phosphatase activity.
J. Leukoc. Biol. 82: 742-51, 2007.
40)  Kruhlak M, Crouch EE, Orlov M, Montaño C, Gorski SA, Nussenzweig A, Misteli T, Phair RD, Casellas R.
The ATM repair pathway inhibits RNA polymerase I transcription in response to chromosome breaks.
Nature. 447: 730-4, 2007.
41)  Kruhlak MJ, Celeste A, Dellaire G, Fernandez-Capetillo O, Müller WG, McNally JG, Bazett-Jones DP, Nussenzweig A.
Changes in chromatin structure and mobility in living cells at sites of DNA double-strand breaks.
J. Cell Biol. 172: 823-34, 2006.
42)  Herbeuval JP, Nilsson J, Boasso A, Hardy AW, Kruhlak MJ, Anderson SA, Dolan MJ, Dy M, Andersson J, Shearer GM.
Differential expression of IFN-alpha and TRAIL/DR5 in lymphoid tissue of progressor versus nonprogressor HIV-1-infected patients.
Proc. Natl. Acad. Sci. U.S.A. 103: 7000-5, 2006.
43)  Kruhlak MJ, Celeste A, Nussenzweig A.
Spatio-temporal dynamics of chromatin containing DNA breaks.
Cell Cycle. 5: 1910-2, 2006.
44)  Kim CS, Vasko VV, Kato Y, Kruhlak M, Saji M, Cheng SY, Ringel MD.
AKT activation promotes metastasis in a mouse model of follicular thyroid carcinoma.
Endocrinology. 146: 4456-63, 2005.
45)  Difilippantonio S, Celeste A, Fernandez-Capetillo O, Chen HT, Reina San Martin B, Van Laethem F, Yang YP, Petukhova GV, Eckhaus M, Feigenbaum L, Manova K, Kruhlak M, Camerini-Otero RD, Sharan S, Nussenzweig M, Nussenzweig A.
Role of Nbs1 in the activation of the Atm kinase revealed in humanized mouse models.
Nat. Cell Biol. 7: 675-85, 2005.
46)  Sarkar K, Kruhlak MJ, Erlandsen SL, Shaw S.
Selective inhibition by rottlerin of macropinocytosis in monocyte-derived dendritic cells.
Immunology. 116: 513-24, 2005.
47)  Vasko V, Saji M, Hardy E, Kruhlak M, Larin A, Savchenko V, Miyakawa M, Isozaki O, Murakami H, Tsushima T, Burman KD, De Micco C, Ringel MD.
Akt activation and localisation correlate with tumour invasion and oncogene expression in thyroid cancer.
J. Med. Genet. 41: 161-70, 2004.
48)  Nijhara R, van Hennik PB, Gignac ML, Kruhlak MJ, Hordijk PL, Delon J, Shaw S.
Rac1 mediates collapse of microvilli on chemokine-activated T lymphocytes.
J. Immunol. 173: 4985-93, 2004.
49)  Leifer CA, Kennedy MN, Mazzoni A, Lee C, Kruhlak MJ, Segal DM.
TLR9 is localized in the endoplasmic reticulum prior to stimulation.
J. Immunol. 173: 1179-83, 2004.
50)  Brown MJ, Nijhara R, Hallam JA, Gignac M, Yamada KM, Erlandsen SL, Delon J, Kruhlak M, Shaw S.
Chemokine stimulation of human peripheral blood T lymphocytes induces rapid dephosphorylation of ERM proteins, which facilitates loss of microvilli and polarization.
Blood. 102: 3890-9, 2003.
51)  Celeste A, Fernandez-Capetillo O, Kruhlak MJ, Pilch DR, Staudt DW, Lee A, Bonner RF, Bonner WM, Nussenzweig A.
Histone H2AX phosphorylation is dispensable for the initial recognition of DNA breaks.
Nat. Cell Biol. 5: 675-9, 2003.
52)  Puri N, Kruhlak MJ, Whiteheart SW, Roche PA.
Mast cell degranulation requires N-ethylmaleimide-sensitive factor-mediated SNARE disassembly.
J. Immunol. 171: 5345-52, 2003.
53)  Ren Y, Kruhlak MJ, Bazett-Jones DP.
Same serial section correlative light and energy-filtered transmission electron microscopy.
J. Histochem. Cytochem. 51: 605-12, 2003.
54)  Tao M, Kruhlak M, Xia S, Androphy E, Zheng ZM.
Signals that dictate nuclear localization of human papillomavirus type 16 oncoprotein E6 in living cells.
J. Virol. 77: 13232-47, 2003.
55)  Hendzel MJ, Kruhlak MJ, MacLean NA, Boisvert F, Lever MA, Bazett-Jones DP.
Compartmentalization of regulatory proteins in the cell nucleus.
J. Steroid Biochem. Mol. Biol. 76: 9-21, 2001.
56)  Kruhlak MJ, Hendzel MJ, Fischle W, Bertos NR, Hameed S, Yang XJ, Verdin E, Bazett-Jones DP.
Regulation of global acetylation in mitosis through loss of histone acetyltransferases and deacetylases from chromatin.
J. Biol. Chem. 276: 38307-19, 2001.
57)  Boisvert FM, Kruhlak MJ, Box AK, Hendzel MJ, Bazett-Jones DP.
The transcription coactivator CBP is a dynamic component of the promyelocytic leukemia nuclear body.
J. Cell Biol. 152: 1099-106, 2001.
58)  Spencer CA, Kruhlak MJ, Jenkins HL, Sun X, Bazett-Jones DP.
Mitotic transcription repression in vivo in the absence of nucleosomal chromatin condensation.
J. Cell Biol. 150: 13-26, 2000.
59)  Kruhlak MJ, Lever MA, Fischle W, Verdin E, Bazett-Jones DP, Hendzel MJ.
Reduced mobility of the alternate splicing factor (ASF) through the nucleoplasm and steady state speckle compartments.
J. Cell Biol. 150: 41-51, 2000.
60)  Wang AH, Kruhlak MJ, Wu J, Bertos NR, Vezmar M, Posner BI, Bazett-Jones DP, Yang XJ.
Regulation of histone deacetylase 4 by binding of 14-3-3 proteins.
Mol. Cell. Biol. 20: 6904-12, 2000.
61)  Bazett-Jones DP, Hendzel MJ, Kruhlak MJ.
Stoichiometric analysis of protein- and nucleic acid-based structures in the cell nucleus.
Micron. 30: 151-7, 1999.
62)  Hendzel MJ, Kruhlak MJ, Bazett-Jones DP.
Organization of highly acetylated chromatin around sites of heterogeneous nuclear RNA accumulation.
Mol. Biol. Cell. 9: 2491-507, 1998.
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This page was last updated on 8/4/2014.