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Our Science – Borgnia Website

Mario Juan Borgnia, Ph.D.

Selected Publications

1)  Milne JL, Borgnia MJ, Bartesaghi A, Tran EE, Earl LA, Schauder DM, Lengyel J, Pierson J, Patwardhan A, Subramaniam S.
Cryo-electron microscopy--a primer for the non-microscopist.
FEBS J. 280: 28-45, 2013.
2)  Schauder DM, Kuybeda O, Zhang J, Klymko K, Bartesaghi A, Borgnia MJ, Mayer ML, Subramaniam S.
Glutamate receptor desensitization is mediated by changes in quaternary structure of the ligand binding domain.
Proc. Natl. Acad. Sci. U.S.A. 110: 5921-6, 2013.
3)  Frank GA, Bartesaghi A, Kuybeda O, Borgnia MJ, White TA, Sapiro G, Subramaniam S.
Computational separation of conformational heterogeneity using cryo-electron tomography and 3D sub-volume averaging.
J. Struct. Biol. 178: 165-76, 2012.
4)  Tran EE, Borgnia MJ, Kuybeda O, Schauder DM, Bartesaghi A, Frank GA, Sapiro G, Milne JL, Subramaniam S.
Structural mechanism of trimeric HIV-1 envelope glycoprotein activation.
PLoS Pathog. 8: e1002797, 2012.
5)  Meyerson JR, White TA, Bliss D, Moran A, Bartesaghi A, Borgnia MJ, de la Cruz MJ, Schauder D, Hartnell LM, Nandwani R, Dawood M, Kim B, Kim JH, Sununu J, Yang L, Bhatia S, Subramaniam C, Hurt DE, Gaudreault L, Subramaniam S.
Determination of molecular structures of HIV envelope glycoproteins using cryo-electron tomography and automated sub-tomogram averaging.
J Vis Exp. 2011.
6)  Khursigara CM, Lan G, Neumann S, Wu X, Ravindran S, Borgnia MJ, Sourjik V, Milne J, Tu Y, Subramaniam S.
Lateral density of receptor arrays in the membrane plane influences sensitivity of the E. coli chemotaxis response.
EMBO J. 30: 1719-29, 2011.
7)  White TA, Bartesaghi A, Borgnia MJ, de la Cruz MJ, Nandwani R, Hoxie JA, Bess JW, Lifson JD, Milne JL, Subramaniam S.
Three-dimensional structures of soluble CD4-bound states of trimeric simian immunodeficiency virus envelope glycoproteins determined by using cryo-electron tomography.
J. Virol. 85: 12114-23, 2011.
8)  Harris A, Borgnia MJ, Shi D, Bartesaghi A, He H, Pejchal R, Kang YK, Depetris R, Marozsan AJ, Sanders RW, Klasse PJ, Milne JL, Wilson IA, Olson WC, Moore JP, Subramaniam S.
Trimeric HIV-1 glycoprotein gp140 immunogens and native HIV-1 envelope glycoproteins display the same closed and open quaternary molecular architectures.
Proc. Natl. Acad. Sci. U.S.A. 108: 11440-5, 2011.
9)  White TA, Bartesaghi A, Borgnia MJ, Meyerson JR, de la Cruz MJ, Bess JW, Nandwani R, Hoxie JA, Lifson JD, Milne JL, Subramaniam S.
Molecular architectures of trimeric SIV and HIV-1 envelope glycoproteins on intact viruses: strain-dependent variation in quaternary structure.
PLoS Pathog. 6: e1001249, 2010.
10)  Narasimha R, Aganj I, Bennett AE, Borgnia MJ, Zabransky D, Sapiro G, McLaughlin SW, Milne JL, Subramaniam S.
Evaluation of denoising algorithms for biological electron tomography.
J. Struct. Biol. 164: 7-17, 2008.
11)  Liu J, Bartesaghi A, Borgnia MJ, Sapiro G, Subramaniam S.
Molecular architecture of native HIV-1 gp120 trimers.
Nature. 455: 109-13, 2008.
12)  Borgnia MJ, Subramaniam S, Milne JL.
Three-dimensional imaging of the highly bent architecture of Bdellovibrio bacteriovorus by using cryo-electron tomography.
J. Bacteriol. 190: 2588-96, 2008.
13)  Milne JL, Wu X, Borgnia MJ, Lengyel JS, Brooks BR, Shi D, Perham RN, Subramaniam S.
Molecular structure of a 9-MDa icosahedral pyruvate dehydrogenase subcomplex containing the E2 and E3 enzymes using cryoelectron microscopy.
J. Biol. Chem. 281: 4364-70, 2006.
14)  Borgnia MJ, Shi D, Zhang P, Milne JL.
Visualization of alpha-helical features in a density map constructed using 9 molecular images of the 1.8 MDa icosahedral core of pyruvate dehydrogenase.
J. Struct. Biol. 147: 136-45, 2004.
15)  Wu X, Milne JL, Borgnia MJ, Rostapshov AV, Subramaniam S, Brooks BR.
A core-weighted fitting method for docking atomic structures into low-resolution maps: application to cryo-electron microscopy.
J. Struct. Biol. 141: 63-76, 2003.
16)  Zhang P, Borgnia MJ, Mooney P, Shi D, Pan M, O'Herron P, Mao A, Brogan D, Milne JL, Subramaniam S.
Automated image acquisition and processing using a new generation of 4K x 4K CCD cameras for cryo electron microscopic studies of macromolecular assemblies.
J. Struct. Biol. 143: 135-44, 2003.
17)  Pohl P, Saparov SM, Borgnia MJ, Agre P.
Highly selective water channel activity measured by voltage clamp: analysis of planar lipid bilayers reconstituted with purified AqpZ.
Proc. Natl. Acad. Sci. U.S.A. 98: 9624-9, 2001.
18)  Borgnia MJ, Agre P.
Reconstitution and functional comparison of purified GlpF and AqpZ, the glycerol and water channels from Escherichia coli.
Proc. Natl. Acad. Sci. U.S.A. 98: 2888-93, 2001.
19)  Braun T, Philippsen A, Wirtz S, Borgnia MJ, Agre P, Kühlbrandt W, Engel A, Stahlberg H.
The 3.7 A projection map of the glycerol facilitator GlpF: a variant of the aquaporin tetramer.
EMBO Rep. 1: 183-9, 2000.
20)  Borgnia M, Nielsen S, Engel A, Agre P.
Cellular and molecular biology of the aquaporin water channels.
Annu. Rev. Biochem. 68: 425-58, 1999.
21)  Borgnia M, Nielsen S, Engel A, Agre P.
Cellular and molecular biology of the aquaporin water channels.
Annu. Rev. Biochem. 68: 425-58, 1999.
22)  Borgnia MJ, Kozono D, Calamita G, Maloney PC, Agre P.
Functional reconstitution and characterization of AqpZ, the E. coli water channel protein.
J. Mol. Biol. 291: 1169-79, 1999.
23)  Ringler P, Borgnia MJ, Stahlberg H, Maloney PC, Agre P, Engel A.
Structure of the water channel AqpZ from Escherichia coli revealed by electron crystallography.
J. Mol. Biol. 291: 1181-90, 1999.
24)  Agre P, Bonhivers M, Borgnia MJ.
The aquaporins, blueprints for cellular plumbing systems.
J. Biol. Chem. 273: 14659-62, 1998.
25)  Borgnia MJ, Eytan GD, Assaraf YG.
Competition of hydrophobic peptides, cytotoxic drugs, and chemosensitizers on a common P-glycoprotein pharmacophore as revealed by its ATPase activity.
J. Biol. Chem. 271: 3163-71, 1996.
26)  Assaraf YG, Borgnia MJ.
Probing the interaction of the multidrug-resistance phenotype with the polypeptide ionophore gramicidin D via functional channel formation.
Eur. J. Biochem. 222: 813-24, 1994.
27)  Eytan GD, Borgnia MJ, Regev R, Assaraf YG.
Transport of polypeptide ionophores into proteoliposomes reconstituted with rat liver P-glycoprotein.
J. Biol. Chem. 269: 26058-65, 1994.
28)  Assaraf YG, Borgnia MJ.
Differential reversal of lipophilic antifolate resistance in mammalian cells with modulators of the multidrug resistance phenotype.
Anticancer Drugs. 4: 395-406, 1993.
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This page was last updated on 8/30/2013.