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Our Science – Waugh Website

David S. Waugh, Ph.D.

Selected Publications

1)  Hogan M, Bahta M, Cherry S, Lountos GT, Tropea JE, Zhao BM, Burke TR, Waugh DS, Ulrich RG.
Biomolecular Interactions of small-molecule inhibitors affecting the YopH protein tyrosine phosphatase.
Chem Biol Drug Des. 81: 323-33, 2013.
[Journal]
2)  Raran-Kurussi S, Tözsér J, Cherry S, Tropea JE, Waugh DS.
Differential temperature dependence of tobacco etch virus and rhinovirus 3C proteases.
Anal. Biochem. 436: 142-4, 2013.
[Journal]
3)  Court DL, Gan J, Liang YH, Shaw GX, Tropea JE, Costantino N, Waugh DS, Ji X.
RNase III: Genetics and function; structure and mechanism.
Annu. Rev. Genet. 47: 405-31, 2013.
[Journal]
4)  Lountos GT, Tropea JE, Waugh DS.
Structure of the Trypanosoma cruzi protein tyrosine phosphatase TcPTP1, a potential therapeutic target for Chagas" disease.
Mol. Biochem. Parasitol. 187: 1-8, 2013.
[Journal]
5)  Austin BP, Waugh DS.
Isolation of Metarhizium anisopliae carboxypeptidase A with native disulfide bonds from the cytosol of Escherichia coli BL21(DE3).
Protein Expr. Purif. 82: 116-24, 2012.
[Journal]
6)  Lountos GT, Tropea JE, Waugh DS.
Structure of the cytoplasmic domain of Yersinia pestis YscD, an essential component of the type III secretion system.
Acta Crystallogr. D Biol. Crystallogr. 68: 201-9, 2012.
[Journal]
7)  Raran-Kurussi S, Waugh DS.
The ability to enhance the solubility of its fusion partners is an intrinsic property of maltose-binding protein but their folding is either spontaneous or chaperone-mediated.
PLoS ONE. 7: e49589, 2012.
[Journal]
8)  Kissner TL, Ruthel G, Alam S, Mann E, Ajami D, Rebek M, Larkin E, Fernandez S, Ulrich RG, Ping S, Waugh DS, Rebek J, Saikh KU.
Therapeutic Inhibition of Pro-Inflammatory Signaling and Toxicity to Staphylococcal Enterotoxin B by a Synthetic Dimeric BB-Loop Mimetic of MyD88.
PLoS ONE. 7: e40773, 2012.
[Journal]
9)  Waugh DS.
An overview of enzymatic reagents for the removal of affinity tags.
Protein Expr. Purif. 80: 283-93, 2011.
[Journal]
10)  Sun P, Tropea JE, Waugh DS.
Enhancing the solubility of recombinant proteins in Escherichia coli by using hexahistidine-tagged maltose-binding protein as a fusion partner.
Methods Mol. Biol. 705: 259-74, 2011.
[Journal]
11)  Kim SE, Bahta M, Lountos GT, Ulrich RG, Burke TR, Waugh DS.
Isothiazolidinone (IZD) as a phosphoryl mimetic in inhibitors of the Yersinia pestis protein tyrosine phosphatase YopH.
Acta Crystallogr. D Biol. Crystallogr. 67: 639-45, 2011.
[Journal]
12)  Waugh DS.
Reprint of: Making the most of affinity tags.
Protein Expr. Purif. [Epub ahead of print], 2011.
[Journal]
13)  Lountos GT, Jobson AG, Tropea JE, Self CR, Zhang G, Pommier Y, Shoemaker RH, Waugh DS.
Structural characterization of inhibitor complexes with checkpoint kinase 2 (Chk2), a drug target for cancer therapy.
J. Struct. Biol. 176: 292-301, 2011.
[Journal]
14)  Lountos GT, Tropea JE, Waugh DS.
Structure of human dual-specificity phosphatase 27 at 2.38 Å resolution.
Acta Crystallogr. D Biol. Crystallogr. 67: 471-9, 2011.
[Journal]
15)  Tu C, Zhou X, Tarasov SG, Tropea JE, Austin BP, Waugh DS, Court DL, Ji X.
The Era GTPase recognizes the GAUCACCUCC sequence and binds helix 45 near the 3' end of 16S rRNA.
Proc Natl Acad Sci U S A. 108: 10156-61, 2011.
[Journal]
16)  Austin BP, Tözsér J, Bagossi P, Tropea JE, Waugh DS.
The substrate specificity of Metarhizium anisopliae and Bos taurus carboxypeptidases A: insights into their use as tools for the removal of affinity tags.
Protein Expr. Purif. 77: 53-61, 2011.
[Journal]
17)  Luke JM, Carnes AE, Sun P, Hodgson CP, Waugh DS, Williams JA.
Thermostable tag (TST) protein expression system: engineering thermotolerant recombinant proteins and vaccines.
J. Biotechnol. 151: 242-50, 2011.
[Journal]
18)  Bahta M, Lountos GT, Dyas B, Kim SE, Ulrich RG, Waugh DS, Burke TR.
Utilization of nitrophenylphosphates and oxime-based ligation for the development of nanomolar affinity inhibitors of the Yersinia pestis outer protein H (YopH) phosphatase.
J. Med. Chem. 54: 2933-43, 2011.
[Journal]
19)  Lountos GT, Jobson AG, Tropea JE, Self CR, Zhang G, Pommier Y, Shoemaker RH, Waugh DS.
X-ray structures of checkpoint kinase 2 in complex with inhibitors that target its gatekeeper-dependent hydrophobic pocket.
FEBS Lett. 585: 3245-9, 2011.
[Journal]
20)  Liu F, Hakami RM, Dyas B, Bahta M, Lountos GT, Waugh DS, Ulrich RG, Burke TR.
A rapid oxime linker-based library approach to identification of bivalent inhibitors of the Yersinia pestis protein-tyrosine phosphatase, YopH.
Bioorg. Med. Chem. Lett. 20: 2813-6, 2010.
[Journal]
21)  Subburaman P, Austin BP, Shaw GX, Waugh DS, Ji X.
Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of macrophage growth locus A (MglA) protein from Francisella tularensis.
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66: 554-7, 2010.
[Journal]
22)  Muus U, Hose C, Yao W, Kosakowska-Cholody T, Farnsworth D, Dyba M, Lountos GT, Waugh DS, Monks A, Burke TR, Michejda CJ.
Development of antiproliferative phenylmaleimides that activate the unfolded protein response.
Bioorg Med Chem. 18: 4535-41, 2010.
[Journal]
23)  Sun P, Austin BP, Tözsér J, Waugh DS.
Structural determinants of tobacco vein mottling virus protease substrate specificity.
Protein Sci. 19: 2240-51, 2010.
[Journal]
24)  Lountos GT, Austin BP, Nallamsetty S, Waugh DS.
Atomic resolution structure of the cytoplasmic domain of Yersinia pestis YscU, a regulatory switch involved in type III secretion.
Protein Sci. 18: 467-74, 2009.
[Journal]
25)  Jobson AG, Lountos GT, Lorenzi PL, Llamas J, Connelly J, Cerna D, Tropea JE, Onda A, Zoppoli G, Kondapaka S, Zhang G, Caplen NJ, Cardellina JH, Yoo SS, Monks A, Self C, Waugh DS, Shoemaker RH, Pommier Y.
Cellular inhibition of checkpoint kinase 2 (Chk2) and potentiation of camptothecins and radiation by the novel Chk2 inhibitor PV1019 [7-nitro-1H-indole-2-carboxylic acid {4-[1-(guanidinohydrazone)-ethyl]-phenyl}-amide].
J. Pharmacol. Exp. Ther. 331: 816-26, 2009.
[Journal]
26)  Lountos GT, Tropea JE, Zhang D, Jobson AG, Pommier Y, Shoemaker RH, Waugh DS.
Crystal structure of checkpoint kinase 2 in complex with NSC 109555, a potent and selective inhibitor.
Protein Sci. 18: 92-100, 2009.
[Journal]
27)  Sun P, Tropea J, Waugh D.
Enhancing the solubility of recombinant proteins in Escherichia coli by using hexahistidine-tagged maltose-binding protein as a fusion partner.
Methods Mol. Biol. 498: 157-72, 2009.
[Journal]
28)  Tropea JE, Cherry S, Waugh DS.
Expression and purification of soluble His(6)-tagged TEV protease.
Methods Mol. Biol. 498: 297-307, 2009.
[Journal]
29)  Austin BP, Nallamsetty S, Waugh DS.
Hexahistidine-tagged maltose-binding protein as a fusion partner for the production of soluble recombinant proteins in Escherichia coli.
Methods Mol. Biol. 498: 157-72, 2009.
[Journal]
30)  Zhang D, Tözsér J, Waugh DS.
Molecular cloning, overproduction, purification and biochemical characterization of the p39 nsp2 protease domains encoded by three alphaviruses.
Protein Expr. Purif. 64: 89-97, 2009.
[Journal]
31)  Lountos G, Tropea J, Cherry S, Waugh D.
Overproduction, purification, and structure determination of human dual-specificity phosphatase 14.
Acta Crystallogr D Biol Crystallogr. 65: 1013-20, 2009.
[Journal]
32)  Tu C, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X.
Structural basis for binding of RNA and cofactor by a KsgA methyltransferase.
Structure. 17: 374-85, 2009.
[Journal]
33)  Tu C, Zhou X, Tropea J, Austin B, Waugh D, Court D, Ji X.
Structure of ERA in complex with the 3' end of 16S rRNA: Implications for ribosome biogenesis.
Proc. Natl. Acad. Sci. USA. 106: 14843-8, 2009.
[Journal]
34)  Lountos G, Jobson A, Tropea J, Zhang D, Self C, Shoemaker R, Pommier Y, Waugh D.
Structure-assisted discovery of novel inhibitors ofhuman checkpoint kinase 2 (Chk2).
Acta Biochim. Pol. 56S: 69-70, 2009.
[Journal]
35)  Gan J, Shaw G, Tropea JE, Waugh DS, Court DL, Ji X.
A stepwise model for double-stranded RNA processing by ribonuclease III.
Mol. Microbiol. 67: 143-54, 2008.
[Journal]
36)  Davis J, Wang J, Tropea JE, Zhang D, Dauter Z, Waugh DS, Wlodawer A.
Novel fold of VirA, a type III secretion system effector protein from Shigella flexneri.
Protein Sci. 17: 2167-73, 2008.
[Journal]
37)  Das R, Loss S, Li J, Waugh DS, Tarasov S, Wingfield PT, Byrd RA, Altieri AS.
Structural biophysics of the NusB:NusE antitermination complex.
J. Mol. Biol. 376: 705-20, 2008.
[Journal]
38)  Sun P, Tropea JE, Austin BP, Cherry S, Waugh DS.
Structural characterization of the Yersinia pestis type III secretion system needle protein YscF in complex with its heterodimeric chaperone YscE/YscG.
J. Mol. Biol. 377: 819-30, 2008.
[Journal]
39)  Tropea JE, Cherry S, Nallamsetty S, Bignon C, Waugh DS.
A generic method for the production of recombinant proteins in Escherichia coli using a dual hexahistidine-maltose-binding protein affinity tag.
Methods Mol. Biol. 363: 1-19, 2007.
[Journal]
40)  Nallamsetty S, Waugh DS.
A generic protocol for the expression and purification of recombinant proteins in Escherichia coli using a combinatorial His6-maltose binding protein fusion tag.
Nat Protoc. 2: 383-91, 2007.
[Journal]
41)  Nallamsetty S, Waugh DS.
Mutations that alter the equilibrium between open and closed conformations of Escherichia coli maltose-binding protein impede its ability to enhance the solubility of passenger proteins.
Biochem. Biophys. Res. Commun. 364: 639-44, 2007.
[Journal]
42)  Sun P, Austin BP, Schubot FD, Waugh DS.
New protein fold revealed by a 1.65 A resolution crystal structure of Francisella tularensis pathogenicity island protein IglC.
Protein Sci. 16: 2560-3, 2007.
[Journal]
43)  Blaszczyk J, Li Y, Cherry S, Alexandratos J, Wu Y, Shaw G, Tropea JE, Waugh DS, Yan H, Ji X.
Structure and activity of Yersinia pestis 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase as a novel target for the development of antiplague therapeutics.
Acta Crystallogr. D Biol. Crystallogr. 63: 1169-77, 2007.
[Journal]
44)  Phan J, Tropea JE, Waugh DS.
Structure-assisted discovery of Variola major H1 phosphatase inhibitors.
Acta Crystallogr. D Biol. Crystallogr. 63: 698-704, 2007.
[Journal]
45)  McFeeters RL, Altieri AS, Cherry S, Tropea JE, Waugh DS, Byrd RA.
The high-precision solution structure of Yersinia modulating protein YmoA provides insight into interaction with H-NS.
Biochemistry. 46: 13975-82, 2007.
[Journal]
46)  Saikh KU, Kissner TL, Dyas B, Tropea JE, Waugh DS, Ulrich RG.
Human cytolytic T cell recognition of Yersinia pestis virulence proteins that target innate immune responses.
J. Infect. Dis. 194: 1753-60, 2006.
[Journal]
47)  Tropea JE, Phan J, Waugh DS.
Overproduction, purification, and biochemical characterization of the dual specificity H1 protein phosphatase encoded by variola major virus.
Protein Expr Purif. 50: 31-36, 2006.
[Journal]
48)  Gan J, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X.
Structural insight into the mechanism of double-stranded RNA processing by ribonuclease III.
Cell. 124: 355-66, 2006.
[Journal]
49)  Schubot FD, Tropea JE, Waugh DS.
Structure of the POZ domain of human LRF, a master regulator of oncogenesis.
Biochem. Biophys. Res. Commun. 351: 1-6, 2006.
[Journal]
50)  Tözsér J, Tropea JE, Cherry S, Bagossi P, Copeland TD, Wlodawer A, Waugh DS.
Comparison of the substrate specificity of two potyvirus proteases.
FEBS J. 272: 514-23, 2005.
[Journal]
51)  Schubot FD, Cherry S, Austin BP, Tropea JE, Waugh DS.
Crystal structure of the protease-resistant core domain of Yersinia pestis virulence factor YopR.
Protein Sci. 14: 1679-83, 2005.
[Journal]
52)  Phan J, Austin BP, Waugh DS.
Crystal structure of the Yersinia type III secretion protein YscE.
Protein Sci. 14: 2759-63, 2005.
[Journal]
53)  Phan J, Shi ZD, Burke TR, Waugh DS.
Crystal structures of a high-affinity macrocyclic peptide mimetic in complex with the Grb2 SH2 domain.
J. Mol. Biol. 353: 104-15, 2005.
[Journal]
54)  Nallamsetty S, Austin BP, Penrose KJ, Waugh DS.
Gateway vectors for the production of combinatorially-tagged His6-MBP fusion proteins in the cytoplasm and periplasm of Escherichia coli.
Protein Sci. 14: 2964-71, 2005.
[Journal]
55)  Gan J, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X.
Intermediate states of ribonuclease III in complex with double-stranded RNA.
Structure. 13: 1435-42, 2005.
[Journal]
56)  Waugh DS.
Making the most of affinity tags.
Trends Biotechnol. 23: 316-320, 2005.
[Journal]
57)  Ferracci F, Schubot FD, Waugh DS, Plano GV.
Selection and characterization of Yersinia pestis YopN mutants that constitutively block Yop secretion.
Mol. Microbiol. 57: 970-987, 2005.
[Journal]
58)  Nallamsetty S, Waugh DS.
Solubility enhancing proteins MBP and NusA play a passive role during the folding of their fusion partners.
Prot. Expr. Purif. 45: 175-182, 2005.
[Journal]
59)  Hansen AM, Gu Y, Li M, Andrykovitch M, Waugh DS, Jin DJ, Ji X.
Structural basis for the function of stringent starvation protein a as a transcription factor.
J. Biol. Chem. 280: 17380-91, 2005.
[Journal]
60)  Lee K, Boovanahalli SK, Nam KY, Kang SU, Lee M, Phan J, Wu L, Waugh DS, Zhang ZY, No KT, Lee JJ, Burke TR.
Synthesis of tripeptides as potent Yersinia protein tyrosine phosphatase inhibitors.
Bioorg. Med. Chem. Lett. 15: 4037-42, 2005.
[Journal]
61)  Schubot FD, Jackson MW, Penrose KJ, Cherry S, Tropea JE, Plano GV, Waugh DS.
Three-dimensional structure of a macromolecular assembly that regulates type III secretion in Yersinia pestis.
J Mol Biol. 346: 1147-61, 2005.
[Journal]
62)  Schubot FD, Waugh DS.
A pivotal role for reductive methylation in the de novo crystallization of a ternary complex composed of Yersinia pestis virulence factors YopN, SycN and YscB.
Acta Crystallogr D Biol Crystallogr. 60: 1981-6, 2004.
[Journal]
63)  Nallamsetty S, Kapust RB, Tözsér J, Cherry S, Tropea JE, Copeland TD, Waugh DS.
Efficient site-specific processing of fusion proteins by tobacco vein mottling virus protease in vivo and in vitro.
Protein Expr Purif. 38: 108-15, 2004.
[Journal]
64)  Liang X, Young JJ, Boone SA, Waugh DS, Duesbery NS.
Involvement of domain II in toxicity of anthrax lethal factor.
J. Biol. Chem. 279: 52473-8, 2004.
[Journal]
65)  Blaszczyk J, Gan J, Tropea JE, Court DL, Waugh DS, Ji X.
Noncatalytic Assembly of Ribonuclease III with Double-Stranded RNA.
Structure (Camb). 12: 457-66, 2004.
[Journal]
66)  Swietnicki W, O'Brien S, Holman K, Cherry S, Brueggemann E, Tropea JE, Hines HB, Waugh DS, Ulrich RG.
Novel protein-protein interactions of the Yersinia pestis type III secretion system elucidated with a matrix analysis by surface plasmon resonance and mass spectrometry.
J Biol Chem. 279: 38693-700, 2004.
[Journal]
67)  Phan J, Tropea JE, Waugh DS.
Structure of the Yersinia pestis type III secretion chaperone SycH in complex with a stable fragment of YscM2.
Acta Crystallogr. D Biol. Crystallogr. 60: 1591-9, 2004.
[Journal]
68)  Derewenda U, Mateja A, Devedjiev Y, Routzahn KM, Evdokimov AG, Derewenda ZS, Waugh DS.
The structure of Yersinia pestis V-antigen, an essential virulence factor and mediator of immunity against plague.
Structure (Camb). 12: 301-6, 2004.
[Journal]
69)  Zhdanov AS, Phan J, Evdokimov AG, Tropea JE, Kapust RB, Li M, Wlodawer A, Waugh DS.
[Tobacco etch virus proteinase: crystal structure of the active enzyme and its inactive mutant].
Bioorg Khim. 29: 457-60, 2003.
[Journal]
70)  Knowlton JR, Bubunenko M, Andrykovitch M, Guo W, Routzahn KM, Waugh DS, Court DL, Ji X.
A spring-loaded state of NusG in its functional cycle is suggested by X-ray crystallography and supported by site-directed mutants.
Biochemistry. 42: 2275-81, 2003.
[Journal]
71)  Andrykovitch M, Routzahn KM, Li M, Gu Y, Waugh DS, Ji X.
Characterization of four orthologs of stringent starvation protein A.
Acta Crystallogr D Biol Crystallogr. 59: 881-6, 2003.
[Journal]
72)  Phan J, Lee K, Cherry S, Tropea JE, Burke TR, Waugh DS.
High-resolution structure of the Yersinia pestis protein tyrosine phosphatase YopH in complex with a phosphotyrosyl mimetic-containing hexapeptide.
Biochemistry. 42: 13113-21, 2003.
[Journal]
73)  Fox JD, Routzahn KM, Bucher MH, Waugh DS.
Maltodextrin-binding proteins from diverse bacteria and archaea are potent solubility enhancers.
FEBS Lett. 537: 53-7, 2003.
[Journal]
74)  Fox JD, Waugh DS.
Maltose-binding protein as a solubility enhancer.
Methods Mol Biol. 205: 99-117, 2003.
[Journal]
75)  Evdokimov AG, Phan J, Tropea JE, Routzahn KM, Peters HK, Pokross M, Waugh DS.
Similar modes of polypeptide recognition by export chaperones in flagellar biosynthesis and type III secretion.
Nat Struct Biol. 10: 789-93, 2003.
[Journal]
76)  Lee K, Gao Y, Yao ZJ, Phan J, Wu L, Liang J, Waugh DS, Zhang ZY, Burke TR.
Tripeptide inhibitors of Yersinia protein-tyrosine phosphatase.
Bioorg Med Chem Lett. 13: 2577-81, 2003.
[Journal]
77)  Evdokimov AG, Tropea JE, Routzahn KM, Waugh DS.
Crystal structure of the Yersinia pestis GTPase activator YopE.
Protein Sci. 11: 401-8, 2002.
[Journal]
78)  Andrykovitch M, Guo W, Routzahn KM, Gu Y, Anderson DE, Reshetnikova LS, Knowlton JR, Waugh DS, Ji X.
Crystallization and preliminary X-ray diffraction studies of NusG, a protein shared by the transcription and translation machines.
Acta Crystallogr D Biol Crystallogr. 58: 2157-8, 2002.
[Journal]
79)  Bucher MH, Evdokimov AG, Waugh DS.
Differential effects of short affinity tags on the crystallization of Pyrococcus furiosus maltodextrin-binding protein.
Acta Crystallogr D Biol Crystallogr. 58: 392-7, 2002.
[Journal]
80)  Routzahn KM, Waugh DS.
Differential effects of supplementary affinity tags on the solubility of MBP fusion proteins.
J. Struct. Funct. Genomics. 2: 83-92, 2002.
[Journal]
81)  Kapust RB, Routzahn KM, Waugh DS.
Processive degradation of nascent polypeptides, triggered by tandem AGA codons, limits the accumulation of recombinant tobacco etch virus protease in Escherichia coli BL21(DE3).
Protein Expr Purif. 24: 61-70, 2002.
[Journal]
82)  Zhou M, Horita DA, Waugh DS, Byrd RA, Morrison DK.
Solution structure and functional analysis of the cysteine-rich C1 domain of kinase suppressor of Ras (KSR).
J Mol Biol. 315: 435-46, 2002.
[Journal]
83)  Phan J, Zdanov A, Evdokimov AG, Tropea JE, Peters HK, Kapust RB, Li M, Wlodawer A, Waugh DS.
Structural basis for the substrate specificity of tobacco etch virus protease.
J Biol Chem. 277: 50564-72, 2002.
[Journal]
84)  Kapust RB, Tözsér J, Copeland TD, Waugh DS.
The P1' specificity of tobacco etch virus protease.
Biochem Biophys Res Commun. 294: 949-55, 2002.
[Journal]
85)  Evdokimov AG, Tropea JE, Routzahn KM, Waugh DS.
Three-dimensional structure of the type III secretion chaperone SycE from Yersinia pestis.
Acta Crystallogr D Biol Crystallogr. 58: 398-406, 2002.
[Journal]
86)  Blaszczyk J, Tropea JE, Bubunenko M, Routzahn KM, Waugh DS, Court DL, Ji X.
Crystallographic and modeling studies of RNase III suggest a mechanism for double-stranded RNA cleavage.
Structure (Camb). 9: 1225-36, 2001.
[Journal]
87)  Fox JD, Kapust RB, Waugh DS.
Single amino acid substitutions on the surface of Escherichia coli maltose-binding protein can have a profound impact on the solubility of fusion proteins.
Protein Sci. 10: 622-30, 2001.
[Journal]
88)  Evdokimov AG, Anderson DE, Routzahn KM, Waugh DS.
Structural basis for oligosaccharide recognition by Pyrococcus furiosus maltodextrin-binding protein.
J Mol Biol. 305: 891-904, 2001.
[Journal]
89)  Evdokimov AG, Tropea JE, Routzahn KM, Copeland TD, Waugh DS.
Structure of the N-terminal domain of Yersinia pestis YopH at 2.0 A resolution.
Acta Crystallogr D Biol Crystallogr. 57: 793-9, 2001.
[Journal]
90)  Kapust RB, Tözsér J, Fox JD, Anderson DE, Cherry S, Copeland TD, Waugh DS.
Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency.
Protein Eng. 14: 993-1000, 2001.
[Journal]
91)  Evdokimov AG, Anderson DE, Routzahn KM, Waugh DS.
Unusual molecular architecture of the Yersinia pestis cytotoxin YopM: a leucine-rich repeat protein with the shortest repeating unit.
J Mol Biol. 312: 807-21, 2001.
[Journal]
92)  Kapust RB, Waugh DS.
Controlled intracellular processing of fusion proteins by TEV protease.
Protein Expr Purif. 19: 312-8, 2000.
[Journal]
93)  Eisenmesser EZ, Kapust RB, Nawrocki JP, Mazzulla MJ, Pannell LK, Waugh DS, Byrd RA.
Expression, purification, refolding, and characterization of recombinant human interleukin-13: utilization of intracellular processing.
Protein Expr Purif. 20: 186-95, 2000.
[Journal]
94)  Evdokimov AG, Anderson DE, Routzahn KM, Waugh DS.
Overproduction, purification, crystallization and preliminary X-ray diffraction analysis of YopM, an essential virulence factor extruded by the plague bacterium Yersinia pestis.
Acta Crystallogr D Biol Crystallogr. 56 Pt 12: 1676-9, 2000.
[Journal]
95)  Kireeva ML, Komissarova N, Waugh DS, Kashlev M.
The 8-nucleotide-long RNA:DNA hybrid is a primary stability determinant of the RNA polymerase II elongation complex.
J Biol Chem. 275: 6530-6, 2000.
[Journal]
96)  Baden HA, Sarma SP, Kapust RB, Byrd RA, Waugh DS.
The amino-terminal domain of human STAT4. Overproduction, purification, and biophysical characterization.
J. Biol. Chem. 273: 17109-14, 1998.
[Journal]
97)  Tsao KL, Waugh DS.
Balancing the production of two recombinant proteins in Escherichia coli by manipulating plasmid copy number: high-level expression of heterodimeric Ras farnesyltransferase.
Protein Expr. Purif. 11: 233-40, 1997.
[Journal]
98)  Waugh DS.
Genetic tools for selective labeling of proteins with alpha-15N-amino acids.
J. Biomol. NMR. 8: 184-92, 1996.
[Journal]
99)  Emerson SD, Madison VS, Palermo RE, Waugh DS, Scheffler JE, Tsao KL, Kiefer SE, Liu SP, Fry DC.
Structure of the Ras-binding domain of c-Raf-1 as determined by NMR spectroscopy and identification of the region that interacts with Ras.
Drug Des Discov. 13: 83-93, 1996.
[Journal]
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This page was last updated on 4/10/2014.