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Our Science – Emmert-Buck Website

Michael R. Emmert-Buck, M.D., Ph.D.

Selected Publications

1)  Armani M, Tangrea M, Yang B, Rosenberg A, Ylaya K, Morris J, Rodriguez-Canales J, Hanson J, Shapiro B, Emmert-Buck MR, Smela E, Hewitt SM.
Multiplex Quantitative Measurement of mRNAs From Fixed Tissue Microarray Sections.
Diagn. Mol. Pathol. [Epub ahead of print], 2014.
2)  Humerick M, Hanson J, Rodriguez-Canales J, Lubelski D, Rashid OM, Salinas YD, Shi Y, Ponzio T, Fields R, Emmert-Buck MR, Gainer H.
Analysis of transcription factor mRNAs in identified oxytocin and vasopressin magnocellular neurons isolated by laser capture microdissection.
PLoS ONE. 8: e69407, 2013.
3)  Li H, Rodriguez-Canales J, Liu W, Zhu J, Hanson JC, Pack S, Zhuang Z, Emmert-Buck MR, Rodgers GP.
Deletion of the olfactomedin 4 gene is associated with progression of human prostate cancer.
Am. J. Pathol. 183: 1329-38, 2013.
4)  Chen Y, Dodd SJ, Tangrea MA, Emmert-Buck MR, Koretsky AP.
Measuring collective cell movement and extracellular matrix interactions using magnetic resonance imaging.
Sci Rep. 3: 1879, 2013.
5)  Rodriguez-Canales J, Hanson JC, Hipp JD, Balis UJ, Tangrea MA, Emmert-Buck MR, Bova GS.
Optimal molecular profiling of tissue and tissue components: defining the best processing and microdissection methods for biomedical applications.
Methods Mol. Biol. 980: 61-120, 2013.
6)  Mukherjee S, Rodriguez-Canales J, Hanson J, Emmert-Buck MR, Tangrea MA, Prieto DA, Blonder J, Johann DJ.
Proteomic analysis of frozen tissue samples using laser capture microdissection.
Methods Mol. Biol. 1002: 71-83, 2013.
7)  Blackler AR, Morgan NY, Gao B, Olano LR, Armani MD, Romantseva E, Kakareka JW, Bonner RF, Mukherjee S, Xiao B, Tran K, Pohida TJ, Emmert-Buck MR, Tangrea MA, Markey SP.
Proteomic analysis of nuclei dissected from fixed rat brain tissue using expression microdissection.
Anal. Chem. 85: 7139-45, 2013.
8)  Yan W, Shih J, Rodriguez-Canales J, Tangrea MA, Player A, Diao L, Hu N, Goldstein AM, Wang J, Taylor PR, Lippman SM, Wistuba II, Emmert-Buck MR, Erickson HS.
Three-dimensional mRNA measurements reveal minimal regional heterogeneity in esophageal squamous cell carcinoma.
Am. J. Pathol. 182: 529-39, 2013.
9)  Du Q, Yan W, Burton VH, Hewitt SM, Wang L, Hu N, Taylor PR, Armani MD, Mukherjee S, Emmert-Buck MR, Tangrea MA.
Validation of esophageal squamous cell carcinoma candidate genes from high-throughput transcriptomic studies.
Am J Cancer Res. 3: 402-10, 2013.
10)  Chowdhuri SR, Xi L, Pham TH, Hanson J, Rodriguez-Canales J, Berman A, Rajan A, Giaccone G, Emmert-Buck M, Raffeld M, Filie AC.
EGFR and KRAS mutation analysis in cytologic samples of lung adenocarcinoma enabled by laser capture microdissection.
Mod. Pathol. 25: 548-55, 2012.
11)  Yan W, Shih JH, Rodriguez-Canales J, Tangrea MA, Ylaya K, Hipp J, Player A, Hu N, Goldstein AM, Taylor PR, Emmert-Buck MR, Erickson HS.
Identification of unique expression signatures and therapeutic targets in esophageal squamous cell carcinoma.
BMC Res Notes. 5: 73, 2012.
12)  Hipp J, Monaco J, Kunju LP, Cheng J, Yagi Y, Rodriguez-Canales J, Emmert-Buck MR, Hewitt S, Feldman MD, Tomaszewski JE, Toner M, Tompkins RG, Flotte T, Lucas D, Gilbertson JR, Madabhushi A, Balis U.
Integration of architectural and cytologic driven image algorithms for prostate adenocarcinoma identification.
Anal Cell Pathol (Amst). 35:: 251-65, 2012.
13)  Roy Chowdhuri S, Hanson J, Cheng J, Rodriguez-Canales J, Fetsch P, Balis U, Filie AC, Giaccone G, Emmert-Buck MR, Hipp JD.
Semiautomated laser capture microdissection of lung adenocarcinoma cytology samples.
Acta Cytol. 56: 622-31, 2012.
14)  Nacev A, Kim SH, Rodriguez-Canales J, Tangrea MA, Shapiro B, Emmert-Buck MR.
A dynamic magnetic shift method to increase nanoparticle concentration in cancer metastases: a feasibility study using simulations on autopsy specimens.
Int J Nanomedicine. 6: 2907-23, 2011.
15)  Antin JH, Aplin AE, Cheng SY, Emmert-Buck MR, Cryns VL, Wang D.
American journal of cancer research: editorial board (2011).
Am J Cancer Res. 1: 817-22, 2011.
16)  Emmert-Buck MR.
An NIH intramural percubator as a model of academic-industry partnerships: from the beginning of life through the valley of death.
J Transl Med. 9: 54, 2011.
17)  Hipp J, Flotte T, Monaco J, Cheng J, Madabhushi A, Yagi Y, Rodriguez-Canales J, Emmert-Buck M, Dugan MC, Hewitt S, Toner M, Tompkins RG, Lucas D, Gilbertson JR, Balis UJ.
Computer aided diagnostic tools aim to empower rather than replace pathologists: Lessons learned from computational chess.
J Pathol Inform. 2: 25, 2011.
18)  Gannot G, Richardson AM, Rodriguez-Canales J, Pinto PA, Merino MJ, Chuaqui RF, Gillespie JW, Emmert-Buck MR.
Decrease in CD8+ lymphocyte number and altered cytokine profile in human prostate cancer.
Am J Cancer Res. 1: 120-127, 2011.
19)  Hipp JD, Lucas DR, Emmert-Buck MR, Compton CC, Balis UJ.
Digital slide repositories for publications: lessons learned from the microarray community.
Am. J. Surg. Pathol. 35: 783-6, 2011.
20)  Tangrea MA, Mukherjee S, Gao B, Markey SP, Du Q, Armani M, Kreitman MS, Rosenberg AM, Wallis BS, Eberle FC, Duncan FC, Hanson JC, Chuaqui RF, Rodriguez-Canales J, Emmert-Buck MR.
Effect of immunohistochemistry on molecular analysis of tissue samples: implications for microdissection technologies.
J. Histochem. Cytochem. 59: 591-600, 2011.
21)  Hanson JC, Tangrea MA, Kim S, Armani MD, Pohida TJ, Bonner RF, Rodriguez-Canales J, Emmert-Buck MR.
Expression microdissection adapted to commercial laser dissection instruments.
Nat Protoc. 6: 457-67, 2011.
22)  Hipp J, Cheng J, Pantanowitz L, Hewitt S, Yagi Y, Monaco J, Madabhushi A, Rodriguez-Canales J, Hanson J, Roy-Chowdhuri S, Filie AC, Feldman MD, Tomaszewski JE, Shih NN, Brodsky V, Giaccone G, Emmert-Buck MR, Balis UJ.
Image microarrays (IMA): Digital pathology's missing tool.
J Pathol Inform. 2: 47, 2011.
23)  Tangrea MA, Hanson JC, Bonner RF, Pohida TJ, Rodriguez-Canales J, Emmert-Buck MR.
Immunoguided microdissection techniques.
Methods Mol. Biol. 755: 57-66, 2011.
24)  Antin JH, Emmert-Buck MR, Aplin AE, Cheng SY, Cryns VL, Wang D.
Launching of American Journal of Cancer Research.
Am J Cancer Res. 1: i, 2011.
25)  Zhu L, Tangrea MA, Mukherjee S, Emmert-Buck MR.
Layered electrophoretic transfer - A method for pre-analytic processing of histological sections.
Proteomics. 11: 883-9, 2011.
26)  Eberle FC, Rodriguez-Canales J, Wei L, Hanson JC, Killian JK, Sun HW, Adams LG, Hewitt SM, Wilson WH, Pittaluga S, Meltzer PS, Staudt LM, Emmert-Buck MR, Jaffe ES.
Methylation profiling of mediastinal gray zone lymphoma reveals a distinctive signature with elements shared by classical Hodgkin's lymphoma and primary mediastinal large B-cell lymphoma.
Haematologica. 96: 558-66, 2011.
27)  Zhu L, Yan W, Rodriguez-Canales J, Rosenberg AM, Hu N, Goldstein AM, Taylor PR, Erickson HS, Emmert-Buck MR, Tangrea MA.
MicroRNA analysis of microdissected normal squamous esophageal epithelium and tumor cells.
Am J Cancer Res. 1: 574-584, 2011.
28)  Armani M, Tangrea MA, Shapiro B, Emmert-Buck MR, Smela E.
Quantifying mRNA levels across tissue sections with 2D-RT-qPCR.
Anal Bioanal Chem. 400: 3383-93, 2011.
29)  Hipp J, Cheng J, Hanson JC, Yan W, Taylor P, Hu N, Rodriguez-Canales J, Hipp J, Tangrea MA, Emmert-Buck MR, Balis U.
SIVQ-aided laser capture microdissection: A tool for high-throughput expression profiling.
J Pathol Inform. 2: 19, 2011.
30)  Yan W, Wistuba II, Emmert-Buck MR, Erickson HS.
Squamous Cell Carcinoma - Similarities and Differences among Anatomical Sites.
Am J Cancer Res. 1: 275-300, 2011.
31)  Rodriguez-Canales J, Eberle FC, Jaffe ES, Emmert-Buck MR.
Why is it crucial to reintegrate pathology into cancer research?.
Bioessays. 33: 490-8, 2011.
32)  Erickson HS, Gannot G, Tangrea MA, Chuaqui RF, Gillespie JW, Emmert-Buck MR.
High throughput screening of normal and neoplastic tissue samples.
Comb. Chem. High Throughput Screen. 13: 253-67, 2010.
33)  Eberle FC, Hanson JC, Killian JK, Wei L, Ylaya K, Hewitt SM, Jaffe ES, Emmert-Buck MR, Rodriguez-Canales J.
Immunoguided laser assisted microdissection techniques for DNA methylation analysis of archival tissue specimens.
J Mol Diagn. 12: 394-401, 2010.
34)  Erickson HS, Josephson JW, Vira M, Albert PS, Gillespie JW, Rodriguez-Canales J, Pinto PA, Chuaqui RF, Emmert-Buck MR, Coleman JA.
Influence of hypoxia induced by minimally invasive prostatectomy on gene expression: implications for biomarker analysis.
Am J Transl Res. 2: 210-22, 2010.
35)  Arnott D, Emmert-Buck MR.
Proteomic profiling of cancer--opportunities, challenges, and context.
J. Pathol. 222: 16-20, 2010.
36)  Armani M, Rodriguez-Canales J, Gillespie J, Tangrea M, Erickson H, Emmert-Buck MR, Shapiro B, Smela E.
2D-PCR: a method of mapping DNA in tissue sections.
Lab Chip. 9: 3526-34, 2009.
37)  Johann DJ, Rodriguez-Canales J, Mukherjee S, Prieto DA, Hanson JC, Emmert-Buck M, Blonder J.
Approaching solid tumor heterogeneity on a cellular basis by tissue proteomics using laser capture microdissection and biological mass spectrometry.
J. Proteome Res. 8: 2310-8, 2009.
38)  Zoon CK, Starker EQ, Wilson AM, Emmert-Buck MR, Libutti SK, Tangrea MA.
Current molecular diagnostics of breast cancer and the potential incorporation of microRNA.
Expert Rev. Mol. Diagn. 9: 455-67, 2009.
39)  Mukherjee S, Richardson AM, Rodriguez-Canales J, Ylaya K, Erickson HS, Player A, Kawasaki ES, Pinto PA, Choyke PL, Merino MJ, Albert PS, Chuaqui RF, Emmert-Buck MR.
Identification of EpCAM as a molecular target of prostate cancer stroma.
Am. J. Pathol. 175: 2277-87, 2009.
40)  Killian JK, Bilke S, Davis S, Walker RL, Killian MS, Jaeger EB, Chen Y, Hipp J, Pittaluga S, Raffeld M, Cornelison R, Smith WI, Bibikova M, Fan JB, Emmert-Buck MR, Jaffe ES, Meltzer PS.
Large-scale profiling of archival lymph nodes reveals pervasive remodeling of the follicular lymphoma methylome.
Cancer Res. 69: 758-64, 2009.
41)  Erickson HS, Albert PS, Gillespie JW, Rodriguez-Canales J, Marston Linehan W, Pinto PA, Chuaqui RF, Emmert-Buck MR.
Quantitative RT-PCR gene expression analysis of laser microdissected tissue samples.
Nat Protoc. 4: 902-22, 2009.
42)  Shou JZ, Hu N, Takikita M, Roth MJ, Johnson LL, Giffen C, Wang QH, Wang C, Wang Y, Su H, Kong LH, Emmert-Buck MR, Goldstein AM, Hewitt SM, Taylor PR.
Overexpression of CDC25B and LAMC2 mRNA and protein in esophageal squamous cell carcinomas and premalignant lesions in subjects from a high-risk population in China.
Cancer Epidemiol. Biomarkers Prev. 17: 1424-35, 2008.
43)  Xiao Y, Gao X, Gannot G, Emmert-Buck MR, Srivastava S, Wagner PD, Amos MD, Barker PE.
Quantitation of HER2 and telomerase biomarkers in solid tumors with IgY antibodies and nanocrystal detection.
Int. J. Cancer. 122: 2178-86, 2008.
44)  Blonder J, Johann DJ, Veenstra TD, Xiao Z, Emmert-Buck MR, Ziegler RG, Rodriguez-Canales J, Hanson JA, Xu X.
Quantitation of steroid hormones in thin fresh frozen tissue sections.
Anal. Chem. 80: 8845-52, 2008.
45)  Erickson HS, Gillespie JW, Emmert-Buck MR.
Tissue microdissection.
Methods Mol. Biol. 424: 433-48, 2008.
46)  Erickson HS, Albert PS, Gillespie JW, Wallis BS, Rodriguez-Canales J, Linehan WM, Gonzalez S, Velasco A, Chuaqui RF, Emmert-Buck MR.
Assessment of normalization strategies for quantitative RT-PCR using microdissected tissue samples.
Lab Invest. 87: 951-62, 2007.
47)  Richardson AM, Woodson K, Wang Y, Rodriguez-Canales J, Erickson HS, Tangrea MA, Novakovic K, Gonzalez S, Velasco A, Kawasaki ES, Emmert-Buck MR, Chuaqui RF, Player A.
Global expression analysis of prostate cancer-associated stroma and epithelia.
Diagn. Mol. Pathol. 16: 189-97, 2007.
48)  Rodriguez-Canales J, Hanson JC, Tangrea MA, Erickson HS, Albert PS, Wallis BS, Richardson AM, Pinto PA, Linehan WM, Gillespie JW, Merino MJ, Libutti SK, Woodson KG, Emmert-Buck MR, Chuaqui RF.
Identification of a unique epigenetic sub-microenvironment in prostate cancer.
J. Pathol. 211: 410-9, 2007.
49)  Catena R, Muniz-Medina V, Moralejo B, Javierre B, Best CJ, Emmert-Buck MR, Green JE, Baker CC, Calvo A.
Increased expression of VEGF121/VEGF165-189 ratio results in a significant enhancement of human prostate tumor angiogenesis.
Int. J. Cancer. 120: 2096-109, 2007.
50)  Gannot G, Tangrea MA, Richardson AM, Flaig MJ, Hewitt SM, Marcus EM, Emmert-Buck MR, Chuaqui RF.
Layered expression scanning: multiplex molecular analysis of diverse life science platforms.
Clin. Chim. Acta. 376: 9-16, 2007.
51)  Gannot G, Tangrea MA, Erickson HS, Pinto PA, Hewitt SM, Chuaqui RF, Gillespie JW, Emmert-Buck MR.
Layered Peptide Array for Multiplex Immunohistochemistry.
J Mol Diagn. 9: 297-304, 2007.
52)  Gannot G, Tangrea MA, Chuaqui RF, Gillespie JW, Emmert-Buck MR.
Layered peptide arrays: a diverse technique for antibody screening of clinical samples.
Ann. N. Y. Acad. Sci. 1098: 451-3, 2007.
53)  Hanson JA, Gillespie JW, Grover A, Tangrea MA, Chuaqui RF, Emmert-Buck MR, Tangrea JA, Libutti SK, Linehan WM, Woodson KG.
Gene promoter methylation in prostate tumor-associated stromal cells.
J. Natl. Cancer Inst. 98: 255-61, 2006.
54)  Hu N, Wang C, Hu Y, Yang HH, Kong LH, Lu N, Su H, Wang QH, Goldstein AM, Buetow KH, Emmert-Buck MR, Taylor PR, Lee MP.
Genome-wide loss of heterozygosity and copy number alteration in esophageal squamous cell carcinoma using the Affymetrix GeneChip Mapping 10 K array.
BMC Genomics. 7: 299, 2006.
55)  Grover AC, Tangrea MA, Woodson KG, Wallis BS, Hanson JC, Chuaqui RF, Gillespie JW, Erickson HS, Bonner RF, Pohida TJ, Emmert-Buck MR, Libutti SK.
Tumor-associated endothelial cells display GSTP1 and RARbeta2 promoter methylation in human prostate cancer.
Journal of translational medicine [electronic resource]. 4: 13, 2006.
56)  Hu N, Su H, Li WJ, Giffen C, Goldstein AM, Hu Y, Wang C, Roth MJ, Li G, Dawsey SM, Xu Y, Taylor PR, Emmert-Buck MR.
Allelotyping of esophageal squamous-cell carcinoma on chromosome 13 defines deletions related to family history.
Genes Chromosomes Cancer. 44: 271-8, 2005.
57)  Gannot G, Gillespie JW, Chuaqui RF, Tangrea MA, Linehan WM, Emmert-Buck MR.
Histomathematical analysis of clinical specimens: challenges and progress.
J. Histochem. Cytochem. 53: 177-85, 2005.
58)  Gannot G, Tangrea MA, Gillespie JW, Erickson HS, Wallis BS, Leakan RA, Knezevic V, Hartmann DP, Chuaqui RF, Emmert-Buck MR.
Layered peptide arrays: high-throughput antibody screening of clinical samples.
The Journal of molecular diagnostics : JMD. 7: 427-36, 2005.
59)  Best CJ, Gillespie JW, Yi Y, Chandramouli GV, Perlmutter MA, Gathright Y, Erickson HS, Georgevich L, Tangrea MA, Duray PH, González S, Velasco A, Linehan WM, Matusik RJ, Price DK, Figg WD, Emmert-Buck MR, Chuaqui RF.
Molecular alterations in primary prostate cancer after androgen ablation therapy.
Clin. Cancer Res. 11: 6823-34, 2005.
60)  Tangrea MA, Chuaqui RF, Gillespie JW, Ahram M, Gannot G, Wallis BS, Best CJ, Linehan WM, Liotta LA, Pohida TJ, Bonner RF, Emmert-Buck MR.
Expression Microdissection: Operator-Independent Retrieval of Cells for Molecular Profiling.
Diagn Mol Pathol. 13(4): 207-212, 2004.
61)  Hu N, Goldstein AM, Albert PS, Giffen C, Tang ZZ, Ding T, Taylor PR, Emmert-Buck MR.
Evidence for a familial esophageal cancer susceptibility gene on chromosome 13.
Cancer Epidemiol Biomarkers Prev. 12: 1112-5, 2003.
62)  Chuaqui RF, Bonner RF, Best CJ, Gillespie JW, Flaig MJ, Hewitt SM, Phillips JL, Krizman DB, Tangrea MA, Ahram M, Linehan WM, Knezevic V, Emmert-Buck MR.
Post-analysis follow-up and validation of microarray experiments.
Nat Genet. 32: 509-14, 2002.
63)  Crabtree JS, Scacheri PC, Ward JM, Garrett-Beal L, Emmert-Buck MR, Edgemon KA, Lorang D, Libutti SK, Chandrasekharappa SC, Marx SJ, Spiegel AM, Collins FS.
A mouse model of multiple endocrine neoplasia, type 1, develops multiple endocrine tumors.
Proc Natl Acad Sci U S A. 98: 1118-23, 2001.
64)  Englert CR, Baibakov GV, Emmert-Buck MR.
Layered expression scanning: rapid molecular profiling of tumor samples.
Cancer Res. 60: 1526-30, 2000.
65)  Emmert-Buck MR, Strausberg RL, Krizman DB, Bonaldo MF, Bonner RF, Bostwick DG, Brown MR, Buetow KH, Chuaqui RF, Cole KA, Duray PH, Englert CR, Gillespie JW, Greenhut S, Grouse L, Hillier LW, Katz KS, Klausner RD, Kuznetzov V, Lash AE, Lennon G, Linehan WM, Liotta LA, Marra MA, Munson PJ, Ornstein DK, Prabhu VV, Prange C, Schuler GD, Soares MB, Tolstoshev CM, Vocke CD, Waterston RH.
Molecular profiling of clinical tissue specimens: feasibility and applications.
Am J Pathol. 156: 1109-15, 2000.
66)  Strausberg RL, Buetow KH, Emmert-Buck MR, Klausner RD.
The cancer genome anatomy project: building an annotated gene index.
Trends Genet. 16: 103-6, 2000.
67)  Cole KA, Krizman DB, Emmert-Buck MR.
The genetics of cancer--a 3D model.
Nat Genet. 21: 38-41, 1999.
68)  Bonner RF, Emmert-Buck M, Cole K, Pohida T, Chuaqui R, Goldstein S, Liotta LA.
Laser capture microdissection: molecular analysis of tissue.
Science. 278: 1481,1483, 1997.
69)  Emmert-Buck MR, Lubensky IA, Dong Q, Manickam P, Guru SC, Kester MB, Olufemi SE, Agarwal S, Burns AL, Spiegel AM, Collins FS, Marx SJ, Zhuang Z, Liotta LA, Chandrasekharappa SC, Debelenko LV.
Localization of the multiple endocrine neoplasia type I (MEN1) gene based on tumor loss of heterozygosity analysis.
Cancer Res. 57: 1855-8, 1997.
70)  Chandrasekharappa SC, Guru SC, Manickam P, Olufemi SE, Collins FS, Emmert-Buck MR, Debelenko LV, Zhuang Z, Lubensky IA, Liotta LA, Crabtree JS, Wang Y, Roe BA, Weisemann J, Boguski MS, Agarwal SK, Kester MB, Kim YS, Heppner C, Dong Q, Spiegel AM, Burns AL, Marx SJ.
Positional cloning of the gene for multiple endocrine neoplasia-type 1.
Science. 276: 404-7, 1997.
71)  Emmert-Buck MR, Bonner RF, Smith PD, Chuaqui RF, Zhuang Z, Goldstein SR, Weiss RA, Liotta LA.
Laser capture microdissection.
Science. 274: 998-1001, 1996.
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This page was last updated on 4/28/2014.