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Our Science – Milne Website

Jacqueline L. Milne, Ph.D.

Selected Publications

1)  Milne JL, Borgnia MJ, Bartesaghi A, Tran EE, Earl LA, Schauder DM, Lengyel J, Pierson J, Patwardhan A, Subramaniam S.
Cryo-electron microscopy--a primer for the non-microscopist.
FEBS J. 280: 28-45, 2013.
[Journal]
2)  Harris AK, Bartesaghi A, Milne JL, Subramaniam S.
HIV-1 envelope glycoprotein trimers display open quaternary conformation when bound to the gp41 MPER-directed broadly neutralizing antibody Z13e1.
J. Virol. [Epub ahead of print], 2013.
[Journal]
3)  Tran EE, Borgnia MJ, Kuybeda O, Schauder DM, Bartesaghi A, Frank GA, Sapiro G, Milne JL, Subramaniam S.
Structural Mechanism of Trimeric HIV-1 Envelope Glycoprotein Activation.
PLoS Pathog. 8: e1002797, 2012.
[Journal]
4)  Khursigara CM, Lan G, Neumann S, Wu X, Ravindran S, Borgnia MJ, Sourjik V, Milne J, Tu Y, Subramaniam S.
Lateral density of receptor arrays in the membrane plane influences sensitivity of the E. coli chemotaxis response.
EMBO J. 30: 1719-29, 2011.
[Journal]
5)  Butan C, Hartnell LM, Fenton AK, Bliss D, Sockett RE, Subramaniam S, Milne JL.
Spiral architecture of the nucleoid in Bdellovibrio bacteriovorus.
J. Bacteriol. 193: 1341-50, 2011.
[Journal]
6)  White TA, Bartesaghi A, Borgnia MJ, de la Cruz MJ, Nandwani R, Hoxie JA, Bess JW, Lifson JD, Milne JL, Subramaniam S.
Three-dimensional structures of soluble CD4-bound states of trimeric simian immunodeficiency virus envelope glycoproteins determined by using cryo-electron tomography.
J. Virol. 85: 12114-23, 2011.
[Journal]
7)  Harris A, Borgnia MJ, Shi D, Bartesaghi A, He H, Pejchal R, Kang YK, Depetris R, Marozsan AJ, Sanders RW, Klasse PJ, Milne JL, Wilson IA, Olson WC, Moore JP, Subramaniam S.
Trimeric HIV-1 glycoprotein gp140 immunogens and native HIV-1 envelope glycoproteins display the same closed and open quaternary molecular architectures.
Proc. Natl. Acad. Sci. U.S.A. 108: 11440-5, 2011.
[Journal]
8)  Milne J.
Structure and regulation of pyruvate dehydrogenase complexes. In: Encyclopedia of Biological Chemistry.
M. Daniel Lane and William Lennarz, eds. Elsevier Inc.; 2010. In Press. [Book Chapter]
9)  White TA, Bartesaghi A, Borgnia MJ, Meyerson JR, de la Cruz MJ, Bess JW, Nandwani R, Hoxie JA, Lifson JD, Milne JL, Subramaniam S.
Molecular architectures of trimeric SIV and HIV-1 envelope glycoproteins on intact viruses: strain-dependent variation in quaternary structure.
PLoS Pathog. 6: e1001249, 2010.
[Journal]
10)  Hildebrand M, Kim S, Shi D, Scott K, Subramaniam S.
3D imaging of diatoms with ion-abrasion scanning electron microscopy.
J. Struct. Biol. 166: 316-28, 2009.
[Journal]
11)  Heymann JA, Shi D, Kim S, Bliss D, Milne JL, Subramaniam S.
3D imaging of mammalian cells with ion-abrasion scanning electron microscopy.
J. Struct. Biol. 166: 1-7, 2009.
[Journal]
12)  Milne JL, Subramaniam S.
Cryo-electron tomography of bacteria: progress, challenges and future prospects.
Nat. Rev. Microbiol. 7: 666-75, 2009.
[Journal]
13)  Lengyel JS, Milne JL, Subramaniam S.
Electron tomography in nanoparticle imaging and analysis.
Nanomedicine (Lond). 3: 125-31, 2008.
[Journal]
14)  Narasimha R, Aganj I, Bennett AE, Borgnia MJ, Zabransky D, Sapiro G, McLaughlin SW, Milne JL, Subramaniam S.
Evaluation of denoising algorithms for biological electron tomography.
J. Struct. Biol. 164: 7-17, 2008.
[Journal]
15)  Lengyel JS, Stott KM, Wu X, Brooks BR, Balbo A, Schuck P, Perham RN, Subramaniam S, Milne JL.
Extended polypeptide linkers establish the spatial architecture of a pyruvate dehydrogenase multienzyme complex.
Structure. 16: 93-103, 2008.
[Journal]
16)  Liu J, Bartesaghi A, Borgnia MJ, Sapiro G, Subramaniam S.
Molecular architecture of native HIV-1 gp120 trimers.
Nature. 455: 109-13, 2008.
[Journal]
17)  Nanavati D, Gucek M, Milne JL, Subramaniam S, Markey SP.
Stoichiometry and absolute quantification of proteins with mass spectrometry using fluorescent and isotope-labeled concatenated peptide standards.
Mol. Cell Proteomics. 7: 442-7, 2008.
[Journal]
18)  Borgnia MJ, Subramaniam S, Milne JL.
Three-dimensional imaging of the highly bent architecture of Bdellovibrio bacteriovorus by using cryo-electron tomography.
J. Bacteriol. 190: 2588-96, 2008.
[Journal]
19)  Milne JL, Wu X, Borgnia MJ, Lengyel JS, Brooks BR, Shi D, Perham RN, Subramaniam S.
Molecular structure of a 9-MDa icosahedral pyruvate dehydrogenase subcomplex containing the E2 and E3 enzymes using cryoelectron microscopy.
J. Biol. Chem. 281: 4364-70, 2006.
[Journal]
20)  Subramaniam S, Milne JLS.
Three-dimensional electron microscopy at molecular resolution.
Ann. Rev. Biophys. Biomol. Struct. 33: 141-155, 2004.
[Journal]
21)  Subramaniam S, Milne JL.
Three-dimensional electron microscopy at molecular resolution.
Annu Rev Biophys Biomol Struct. 33: 141-55, 2004.
[Journal]
22)  Borgnia MJ, Shi D, Zhang P, Milne JL.
Visualization of alpha-helical features in a density map constructed using 9 molecular images of the 1.8 MDa icosahedral core of pyruvate dehydrogenase.
J. Struct. Biol. 147: 136-145, 2004.
[Journal]
23)  Wu X, Milne JLS, Borgnia MJ, Rostapshov AV, Subramaniam S, Brooks BR.
A core-weighted fitting method for docking atomic structures into low resolution maps: Applications in cryo-electron microscopy.
J. Struct. Biol. 141: 63-76, 2003.
[Journal]
24)  Wu X, Milne JL, Borgnia MJ, Rostapshov AV, Subramaniam S, Brooks BR.
A core-weighted fitting method for docking atomic structures into low-resolution maps: application to cryo-electron microscopy.
J. Struct. Biol. 141: 63-76, 2003.
[Journal]
25)  Zhang P, Borgnia MJ, Mooney P, Shi D, Pan M, O'Herron P, Mao A, Brogan D, Milne JL, Subramaniam S.
Automated image acquisition and processing using a new generation of 4K x 4K CCD cameras for cryo-electron microscopic studies of macromolecular complexes.
J. Struct. Biol. 143: 135-144, 2003.
[Journal]
26)  Milne JL, Shi D, Rosenthal PB, Sunshine JS, Domingo GJ, Wu X, Brooks BR, Perham RN, Henderson R, Subramaniam S.
Molecular architecture and mechanism of an icosahedral pyruvate dehydrogenase complex: a multifunctional catalytic machine.
EMBO J. 21: 5587-5598, 2002.
[Journal]
27)  Zhang P, Beatty A, Milne JLS, Subramaniam S.
Automated data collection with a Tecnai 12 electron microscope: applications for molecular imaging by cryo-microscopy.
J. Struct. Biol. 135: 251-261, 2001.
[Journal]
28)  Zhang P, Beatty A, Milne JL, Subramaniam S.
Automated data collection with a Tecnai 12 electron microscope: applications for molecular imaging by cryomicroscopy.
J. Struct. Biol. 135: 251-61, 2001.
[Journal]
29)  Heymann JA, Sarker R, Hirai T, Shi D, Milne JL, Maloney PC, Subramaniam S.
Projection structure and molecular architecture of OxlT, a bacterial membrane transporter.
EMBO J. 20: 4408-13, 2001.
[Journal]
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This page was last updated on 5/9/2013.