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Donald L. Court, Ph.D.

Selected Publications

1)  Tal A, Arbel-Goren R, Costantino N, Court DL, Stavans J.
Location of the unique integration site on an Escherichia coli chromosome by bacteriophage lambda DNA in vivo.
Proc. Natl. Acad. Sci. U.S.A. 111: 7308-12, 2014.
2)  Thomason LC, Sawitzke JA, Li X, Costantino N, Court DL.
Recombineering: genetic engineering in bacteria using homologous recombination.
Curr Protoc Mol Biol. 106: 1.16.1-1.16.39, 2014.
3)  Parks AR, Court C, Lubkowska L, Jin DJ, Kashlev M, Court DL.
Bacteriophage λ N protein inhibits transcription slippage by Escherichia coli RNA polymerase.
Nucleic Acids Res. 42: 5823-9, 2014.
4)  Haeusser DP, Hoashi M, Weaver A, Brown N, Pan J, Sawitzke JA, Thomason LC, Court DL, Margolin W.
The Kil peptide of bacteriophage λ blocks Escherichia coli cytokinesis via ZipA-dependent inhibition of FtsZ assembly.
PLoS Genet. 10: e1004217, 2014.
5)  Court DL, Gan J, Liang YH, Shaw GX, Tropea JE, Costantino N, Waugh DS, Ji X.
RNase III: Genetics and function; structure and mechanism.
Annu. Rev. Genet. 47: 405-31, 2013.
6)  Li XT, Thomason LC, Sawitzke JA, Costantino N, Court DL.
Positive and negative selection using the tetA-sacB cassette: recombineering and P1 transduction in Escherichia coli.
Nucleic Acids Res. 41: e204, 2013.
7)  Sawitzke JA, Thomason LC, Bubunenko M, Li X, Costantino N, Court DL.
Recombineering: Highly Efficient in vivo Genetic Engineering using Single-strand Oligos.
Meth. Enzymol. 533: 157-77, 2013.
8)  Sawitzke JA, Thomason LC, Bubunenko M, Li X, Costantino N, Court DL.
Recombineering: Using Drug Cassettes to Knock out Genes in vivo.
Meth. Enzymol. 533: 79-102, 2013.
9)  Sawitzke JA, Bubunenko M, Thomason L, Costantino N, Court DL.
Maloy S, Hughes K, eds.
Recombineering: A modern approach to genetic engineering. In: Brenner's Encyclopedia of Genetics.
Oxford: Elsevier/Academic Press; 2013. p. 109-112 [Book Chapter]
10)  Li XT, Thomason LC, Sawitzke JA, Costantino N, Court DL.
Bacterial DNA polymerases participate in oligonucleotide recombination.
Mol. Microbiol. 88: 906-20, 2013.
11)  Arbel-Goren R, Tal A, Friedlander T, Meshner S, Costantino N, Court DL, Stavans J.
Effects of post-transcriptional regulation on phenotypic noise in Escherichia coli.
Nucleic Acids Res. 41: 4825-34, 2013.
12)  Strader MB, Hervey WJ, Costantino N, Fujigaki S, Chen CY, Akal-Strader A, Ihunnah CA, Makusky AJ, Court DL, Markey SP, Kowalak JA.
A Coordinated Proteomic Approach for Identifying Proteins that Interact with the E. coli Ribosomal Protein S12.
J. Proteome Res. 12: 1289-99, 2013.
13)  Zhou YN, Lubkowska L, Hui M, Court C, Chen S, Court DL, Strathern J, Jin DJ, Kashlev M.
Isolation and characterization of RNA polymerase rpoB mutations that alter transcription slippage during elongation in Escherichia coli.
J. Biol. Chem. 288: 2700-10, 2013.
14)  Bubunenko M, Court DL, Al Refaii A, Saxena S, Korepanov A, Friedman DI, Gottesman ME, Alix JH.
Nus transcription elongation factors and RNase III modulate small ribosome subunit biogenesis in Escherichia coli.
Mol. Microbiol. 87: 382-93, 2013.
15)  Strathern JN, Jin DJ, Court DL, Kashlev M.
Isolation and characterization of transcription fidelity mutants.
Biochim Biophys Acta. 1819: 694-9, 2012.
16)  Sawitzke JA, Youngren B, Thomason LC, Baker T, Sengupta M, Court D, Austin S.
The segregation of Escherichia coli minichromosomes constructed in vivo by recombineering.
Plasmid. 67: 148-54, 2012.
17)  Feiss M, Court DL.
Tidona CA, Darai G, eds.
λ-like phages - Siphoviridae. In: The Springer Index of viruses. Volume Part 83. 2nd Edition.
Berlin; Heidelberg; New York: Springer; 2012. p. 1781-1790 [Book Chapter]
18)  Stagno JR, Altieri AS, Bubunenko M, Tarasov SG, Li J, Court DL, Byrd RA, Ji X.
Structural basis for RNA recognition by NusB and NusE in the initiation of transcription antitermination.
Nucleic Acids Res. 39: 7803-15, 2011.
19)  Tu C, Zhou X, Tarasov SG, Tropea JE, Austin BP, Waugh DS, Court DL, Ji X.
The Era GTPase recognizes the GAUCACCUCC sequence and binds helix 45 near the 3' end of 16S rRNA.
Proc. Natl. Acad. Sci. U.S.A. 108: 10156-61, 2011.
20)  Sawitzke JA, Costantino N, Li XT, Thomason LC, Bubunenko M, Court C, Court DL.
Probing cellular processes with oligo-mediated recombination and using the knowledge gained to optimize recombineering.
J. Mol. Biol. 407: 45-59, 2011.
21)  Strader MB, Costantino N, Elkins CA, Chen CY, Patel I, Makusky AJ, Choy JS, Court DL, Markey SP, Kowalak JA.
A Proteomic and Transcriptomic Approach Reveals New Insight into {beta}-methylthiolation of Escherichia coli Ribosomal Protein S12.
Mol. Cell Proteomics. 10: M110.005199, 2011.
22)  Swingle B, Markel E, Costantino N, Bubunenko MG, Cartinhour S, Court DL.
Oligonucleotide recombination in Gram-negative bacteria.
Mol. Microbiol. 75: 138-48, 2010.
23)  Martínez-Trujillo M, Sánchez-Trujillo A, Ceja V, Avila-Moreno F, Bermúdez-Cruz RM, Court D, Montañez C.
Sequences required for transcription termination at the intrinsic lambdatI terminator.
Can. J. Microbiol. 56: 168-77, 2010.
24)  Ninfa AJ, Atkinson M, Forger D, Atkins S, Arps D, Selinsky S, Court D, Perry N, Mayo AE.
Ditty J, Mackey S, Johnson C, eds.
A Synthetic Biology Approach to Understanding Biological Oscillations: Developing a Genetic Oscillator for Escherichia coli. In: Bacterial Circadian Programs. Volume 1.
Berlin: Springer; 2009. p. 301-329. [Book Chapter]
25)  Thomason LC, Oppenheim AB, Court DL.
Modifying bacteriophage lambda with recombineering.
Methods Mol. Biol. 501: 239-51, 2009.
26)  Sharan SK, Thomason LC, Kuznetsov SG, Court DL.
Recombineering: a homologous recombination-based method of genetic engineering.
Nat Protoc. 4: 206-23, 2009.
27)  Tu C, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X.
Structural basis for binding of RNA and cofactor by a KsgA methyltransferase.
Structure. 17: 374-85, 2009.
28)  Tu C, Zhou X, Tropea JE, Austin BP, Waugh DS, Court DL, Ji X.
Structure of ERA in complex with the 3' end of 16S rRNA: Implications for ribosome biogenesis.
Proc. Natl. Acad. Sci. U.S.A. 2009.
29)  Rokney A, Kobiler O, Amir A, Court DL, Stavans J, Adhya S, Oppenheim AB.
Host responses influence on the induction of lambda prophage.
Mol. Microbiol. 68: 29-36, 2008.
30)  Datta S, Costantino N, Zhou X, Court DL.
Identification and analysis of recombineering functions from Gram-negative and Gram-positive bacteria and their phages.
Proc. Natl. Acad. Sci. U.S.A. 105: 1626-31, 2008.
31)  Adhya SL, Court DL, Friedman DI, Gottesman ME.
Obituary: amos oppenheim (31 october 1934 to 24 september 2006).
Mol. Microbiol. 67: 685-6, 2008.
32)  Luo X, Hsiao HH, Bubunenko M, Weber G, Court DL, Gottesman ME, Urlaub H, Wahl MC.
Structural and functional analysis of the E. coli NusB-S10 transcription antitermination complex.
Mol. Cell. 32: 791-802, 2008.
33)  Court DL, Oppenheim AB, Adhya SL.
A new look at bacteriophage lambda genetic networks.
J. Bacteriol. 189: 298-304, 2007.
34)  Chan W, Costantino N, Li R, Lee SC, Su Q, Melvin D, Court DL, Liu P.
A recombineering based approach for high-throughput conditional knockout targeting vector construction.
Nucleic Acids Res. 35: e64, 2007.
35)  Gan J, Shaw G, Tropea JE, Waugh DS, Court DL, Ji X.
A stepwise model for double-stranded RNA processing by ribonuclease III.
Mol Microbiol. 67: 143-154, 2007.
36)  Thomason LC, Costantino N, Court DL.
E. coli genome manipulation by P1 transduction.
Curr Protoc Mol Biol. 79: Unit 1.17, 2007.
Full Text Article. [Journal]
37)  Bubunenko M, Baker T, Court DL.
Essentiality of ribosomal and transcription antitermination proteins analyzed by systematic gene replacement in Escherichia coli.
J. Bacteriol. 189: 2844-53, 2007.
38)  Korepanov AP, Gongadze GM, Garber MB, Court DL, Bubunenko MG.
Importance of the 5 S rRNA-binding ribosomal proteins for cell viability and translation in Escherichia coli.
J. Mol. Biol. 366: 1199-208, 2007.
39)  Thomason LC, Costantino N, Shaw DV, Court DL.
Multicopy plasmid modification with phage lambda Red recombineering.
Plasmid. 58: 148-58, 2007.
40)  Thomason L, Court DL, Bubunenko M, Costantino N, Wilson H, Datta S, Oppenheim A.
Recombineering: Genetic engineering in bacteria using homologous recombination.
Curr Protoc Mol Biol. 78: Unit 1.16, 2007.
Full Text Article. [Journal]
41)  Sawitzke JA, Thomason LC, Costantino N, Bubunenko M, Datta S, Court DL.
Recombineering: in vivo genetic engineering in E. coli, S. enterica, and beyond.
Meth. Enzymol. 421: 171-99, 2007.
42)  Wang Y, Stieglitz KA, Bubunenko M, Court DL, Stec B, Roberts MF.
The structure of the R184A mutant of the inositol monophosphatase encoded by suhB and implications for its functional interactions in E. coli.
J Biol Chem. 282: 26989-96, 2007.
43)  Phadtare S, Kazakov T, Bubunenko M, Court DL, Pestova T, Severinov K.
Transcription antitermination by translation initiation factor IF1.
J. Bacteriol. 189: 4087-93, 2007.
44)  Friedman D, Court D.
Regulation of Gene Expression by Transcription Termination and Antitermination. In: The Bacteriophages.
New York: Oxford University Press; 2006. p. 83-103 [Book Chapter]
45)  Bubunenko M, Korepanov A, Court DL, Jagannathan I, Dickinson D, Chaudhuri BR, Garber MB, Culver GM.
30S ribosomal subunits can be assembled in vivo without primary binding ribosomal protein S15.
RNA. 12: 1229-39, 2006.
46)  Datta S, Costantino N, Court DL.
A set of recombineering plasmids for gram-negative bacteria.
Gene. 379: 109-115, 2006.
47)  Washburn RS, Court DL, Gottesman ME.
Role of an RNase III binding site in transcription termination at lambda nutL by HK022 Nun protein.
J. Bacteriol. 188: 6824-31, 2006.
48)  Gan J, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X.
Structural insight into the mechanism of double-stranded RNA processing by ribonuclease III.
Cell. 124: 355-66, 2006.
49)  Thomason L, Costantino N, Sawitzke J, Datta S, Bubunenko M, Court D, Myers R, Oppenheim A.
Recombineering in Prokaryotes. In: Phages: Their Role in Bacterial Pathogenesis and Biotechnology.
Herndon, VA: ASM Press; 2005. p. 383-399 [Book Chapter]
50)  Gan J, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X.
Intermediate states of ribonuclease III in complex with double-stranded RNA.
Structure. 13: 1435-42, 2005.
51)  Svenningsen SL, Costantino N, Court DL, Adhya S.
On the role of Cro in {lambda} prophage induction.
Proc Natl Acad Sci U S A. 102: 4465-9, 2005.
52)  Kobiler O, Rokney A, Friedman N, Court DL, Stavans J, Oppenheim AB.
Quantitative kinetic analysis of the bacteriophage {lambda} genetic network.
Proc Natl Acad Sci U S A. 102: 4470-5, 2005.
53)  Warming S, Costantino N, Court DL, Jenkins NA, Copeland NG.
Simple and highly efficient BAC recombineering using galK selection.
Nucleic Acids Res. 33: e36, 2005.
54)  Oppenheim AB, Kobiler O, Stavans J, Court DL, Adhya S.
Switches in bacteriophage lambda development.
Annu. Rev. Genet. 39: 409-29, 2005.
55)  Thomason LC, Court DL, Datta AR, Khanna R, Rosner JL.
Identification of the Escherichia coli K-12 ybhE gene as pgl, encoding 6-phosphogluconolactonase.
J Bacteriol. 186: 8248-53, 2004.
56)  Oppenheim AB, Rattray AJ, Bubunenko M, Thomason LC, Court DL.
In vivo recombineering of bacteriophage lambda by PCR fragments and single-strand oligonucleotides.
Virology. 319: 185-9, 2004.
57)  Blaszczyk J, Gan J, Tropea JE, Court DL, Waugh DS, Ji X.
Noncatalytic Assembly of Ribonuclease III with Double-Stranded RNA.
Structure (Camb). 12: 457-66, 2004.
58)  Wilson HR, Zhou JG, Yu D, Court DL.
Translation repression by an RNA polymerase elongation complex.
Mol Microbiol. 53: 821-8, 2004.
59)  Knowlton JR, Bubunenko M, Andrykovitch M, Guo W, Routzahn KM, Waugh DS, Court DL, Ji X.
A spring-loaded state of NusG in its functional cycle is suggested by X-ray crystallography and supported by site-directed mutants.
Biochemistry. 42: 2275-81, 2003.
60)  Costantino N, Court DL.
Enhanced levels of lambda Red-mediated recombinants in mismatch repair mutants.
Proc Natl Acad Sci U S A. 100: 15748-53, 2003.
61)  Li XT, Costantino N, Lu LY, Liu DP, Watt RM, Cheah KS, Court DL, Huang JD.
Identification of factors influencing strand bias in oligonucleotide-mediated recombination in Escherichia coli.
Nucleic Acids Res. 31: 6674-87, 2003.
62)  Court DL, Swaminathan S, Yu D, Wilson H, Baker T, Bubunenko M, Sawitzke J, Sharan SK.
Mini-lambda: a tractable system for chromosome and BAC engineering.
Gene. 315: 63-9, 2003.
63)  Kim HC, Zhou JG, Wilson HR, Mogilnitskiy G, Court DL, Gottesman ME.
Phage HK022 Nun protein represses translation of phage lambda N (transcription termination/translation repression).
Proc Natl Acad Sci U S A. 100: 5308-12, 2003.
64)  Yu D, Sawitzke JA, Ellis H, Court DL.
Recombineering with overlapping single-stranded DNA oligonucleotides: testing a recombination intermediate.
Proc Natl Acad Sci U S A. 100: 7207-12, 2003.
65)  Thomason L, Bubunenko M, Costantino N, Wilson H, Oppenheim A, Court D.
Recombineering: Genetic engineering in bacteria using homologous recombination.
Curr Protoc Mol Biol. 61: Unit 1.16, 2003.
Full Text Article. [Journal]
66)  Feiss M, Court D.
Tidona C, Darai G, eds.
Lambda-like phages. In: The Springer Index of Viruses. Volume 1st edition.
Berlin: Springer-Verlag; 2002. p. 1098-1107 [Book Chapter]
67)  Minkovsky N, Zarimani A, Chary VK, Johnstone BH, Powell BS, Torrance PD, Court DL, Simons RW, Piggot PJ.
Bex, the Bacillus subtilis homolog of the essential Escherichia coli GTPase Era, is required for normal cell division and spore formation.
J Bacteriol. 184: 6389-94, 2002.
68)  Sergueev K, Court D, Reaves L, Austin S.
E. coli cell-cycle regulation by bacteriophage lambda.
J. Mol. Biol. 324: 297-307, 2002.
69)  Court DL, Sawitzke JA, Thomason LC.
Genetic engineering using homologous recombination.
Annu Rev Genet. 36: 361-88, 2002.
70)  Zhou Y, Filter JJ, Court DL, Gottesman ME, Friedman DI.
Requirement for NusG for transcription antitermination in vivo by the lambda N protein.
J Bacteriol. 184: 3416-3418, 2002.
71)  Wilson HR, Yu D, Peters HK, Zhou JG, Court DL.
The global regulator RNase III modulates translation repression by the transcription elongation factor N.
EMBO J. 21: 4154-4161, 2002.
72)  Kobiler O, Koby S, Teff D, Court D, Oppenheim AB.
The phage lambda CII transcriptional activator carries a C-terminal domain signaling for rapid proteolysis.
Proc Natl Acad Sci U S A. 99: 14964-9, 2002.
73)  Lee EC, Yu D, Martinez de Velasco J, Tessarollo L, Swing DA, Court DL, Jenkins NA, Copeland NG.
A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA.
Genomics. 73: 56-65, 2001.
74)  Friedman DI, Court DL.
Bacteriophage lambda: alive and well and still doing its thing.
Curr Opin Microbiol. 4: 201-207, 2001.
75)  Sergueev K, Yu D, Austin S, Court D.
Cell toxicity caused by products of the p(L) operon of bacteriophage lambda.
Gene. 272: 227-235, 2001.
76)  Blaszczyk J, Tropea JE, Bubunenko M, Routzahn KM, Waugh DS, Court DL, Ji X.
Crystallographic and modeling studies of RNase III suggest a mechanism for double-stranded RNA cleavage.
Structure (Camb). 9: 1225-1236, 2001.
77)  Ellis HM, Yu D, DiTizio T, Court DL.
High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides.
Proc Natl Acad Sci U S A. 98: 6742-6746, 2001.
78)  Timmons L, Court DL, Fire A.
Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans.
Gene. 263: 103-112, 2001.
79)  Swaminathan S, Ellis HM, Waters LS, Yu D, Lee EC, Court DL, Sharan SK.
Rapid engineering of bacterial artificial chromosomes using oligonucleotides.
Genesis. 29: 14-21, 2001.
80)  Copeland NG, Jenkins NA, Court DL.
Recombineering: a powerful new tool for mouse functional genomics.
Nat Rev Genet. 2: 769-779, 2001.
81)  Yu D, Ellis HM, Lee EC, Jenkins NA, Copeland NG, Court DL.
An efficient recombination system for chromosome engineering in Escherichia coli.
Proc Natl Acad Sci U S A. 97: 5978-5983, 2000.
82)  Britton RA, Chen SM, Wallis D, Koeuth T, Powell BS, Shaffer LG, Largaespada D, Jenkins NA, Copeland NG, Court DL, Lupski JR.
Isolation and preliminary characterization of the human and mouse homologues of the bacterial cell cycle gene era.
Genomics. 67: 78-82, 2000.
83)  Altieri AS, Mazzulla MJ, Horita DA, Coats RH, Wingfield PT, Das A, Court DL, Byrd RA.
The structure of the transcriptional antiterminator NusB from Escherichia coli.
Nat Struct Biol. 7: 470-474, 2000.
84)  Powell B, Peters HK, Nakamura Y, Court D.
Cloning and analysis of the rnc-era-recO operon from Pseudomonas aeruginosa.
J Bacteriol. 181: 5111-5113, 1999.
85)  Chen X, Court DL, Ji X.
Crystal structure of ERA: a GTPase-dependent cell cycle regulator containing an RNA binding motif.
Proc Natl Acad Sci U S A. 96: 8396-8401, 1999.
86)  Burova E, Hung SC, Chen J, Court DL, Zhou JG, Mogilnitskiy G, Gottesman ME.
Escherichia coli nusG mutations that block transcription termination by coliphage HK022 Nun protein.
Mol Microbiol. 31: 1783-1793, 1999.
87)  Chen X, Chen SM, Powell BS, Court DL, Ji X.
Purification, characterization and crystallization of ERA, an essential GTPase from Escherichia coli.
FEBS Lett. 445: 425-430, 1999.
88)  Yu D, Court DL.
A new system to place single copies of genes, sites and lacZ fusions on the Escherichia coli chromosome.
Gene. 223: 77-81, 1998.
89)  Britton RA, Powell BS, Dasgupta S, Sun Q, Margolin W, Lupski JR, Court DL.
Cell cycle arrest in Era GTPase mutants: a potential growth rate-regulated checkpoint in Escherichia coli.
Mol Microbiol. 27: 739-750, 1998.
90)  Powell BS, Court DL.
Control of ftsZ expression, cell division, and glutamine metabolism in Luria-Bertani medium by the alarmone ppGpp in Escherichia coli.
J Bacteriol. 180: 1053-1062, 1998.
91)  Dasgupta S, Fernandez L, Kameyama L, Inada T, Nakamura Y, Pappas A, Court DL.
Genetic uncoupling of the dsRNA-binding and RNA cleavage activities of the Escherichia coli endoribonuclease RNase III--the effect of dsRNA binding on gene expression.
Mol Microbiol. 28: 629-640, 1998.
92)  Zuber M, Hoover TA, Dertzbaugh MT, Court DL.
A Francisella tularensis DNA clone complements Escherichia coli defective for the production of Era, an essential Ras-like GTP-binding protein.
Gene. 189: 31-34, 1997.
93)  Britton RA, Powell BS, Court DL, Lupski JR.
Characterization of mutations affecting the Escherichia coli essential GTPase era that suppress two temperature-sensitive dnaG alleles.
J Bacteriol. 179: 4575-4582, 1997.
94)  Altieri AS, Mazzulla MJ, Zhou H, Costantino N, Court DL, Byrd RA.
Sequential assignments and secondary structure of the RNA-binding transcriptional regulator NusB.
FEBS Lett. 415: 221-226, 1997.
95)  Wilson HR, Kameyama L, Zhou JG, Guarneros G, Court DL.
Translational repression by a transcriptional elongation factor.
Genes Dev. 11: 2204-2213, 1997.
96)  Das A, Pal M, Mena JG, Whalen W, Wolska K, Crossley R, Rees W, von Hippel PH, Costantino N, Court D, Mazzulla M, Altieri AS, Byrd RA, Chattopadhyay S, DeVito J, Ghosh B.
Components of multiprotein-RNA complex that controls transcription elongation in Escherichia coli phage lambda.
Methods Enzymol. 274: 374-402, 1996.
97)  Cisneros B, Court D, Sanchez A, Montañez C.
Point mutations in a transcription terminator, lambda tI, that affect both transcription termination and RNA stability.
Gene. 181: 127-133, 1996.
98)  Schauer AT, Cheng SW, Zheng C, St Pierre L, Alessi D, Hidayetoglu DL, Costantino N, Court DL, Friedman DI.
The alpha subunit of RNA polymerase and transcription antitermination.
Mol Microbiol. 21: 839-851, 1996.
99)  Zuber M, Hoover TA, Court DL.
Analysis of a Coxiella burnetti gene product that activates capsule synthesis in Escherichia coli: requirement for the heat shock chaperone DnaK and the two-component regulator RcsC.
J Bacteriol. 177: 4238-4244, 1995.
100)  Zuber M, Hoover TA, Dertzbaugh MT, Court DL.
Analysis of the DnaK molecular chaperone system of Francisella tularensis.
Gene. 164: 149-152, 1995.
101)  Zuber M, Hoover TA, Court DL.
Cloning, sequencing and expression of the dnaJ gene of Coxiella burnetii.
Gene. 152: 99-102, 1995.
102)  Powell BS, Court DL, Inada T, Nakamura Y, Michotey V, Cui X, Reizer A, Saier MH, Reizer J.
Novel proteins of the phosphotransferase system encoded within the rpoN operon of Escherichia coli. Enzyme IIANtr affects growth on organic nitrogen and the conditional lethality of an erats mutant.
J Biol Chem. 270: 4822-4839, 1995.
103)  Court DL, Patterson TA, Baker T, Costantino N, Mao X, Friedman DI.
Structural and functional analyses of the transcription-translation proteins NusB and NusE.
J Bacteriol. 177: 2589-2591, 1995.
104)  Friedman DI, Court DL.
Transcription antitermination: the lambda paradigm updated.
Mol Microbiol. 18: 191-200, 1995.
105)  Cheng SW, Court DL, Friedman DI.
Transcription termination signals in the nin region of bacteriophage lambda: identification of Rho-dependent termination regions.
Genetics. 140: 875-887, 1995.
106)  Zuber M, Hoover TA, Powell BS, Court DL.
Analysis of the rnc locus of Coxiella burnetii.
Mol. Microbiol. 14: 291-300, 1994.
107)  Patterson TA, Zhang Z, Baker T, Johnson LL, Friedman DI, Court DL.
Bacteriophage lambda N-dependent transcription antitermination. Competition for an RNA site may regulate antitermination.
J. Mol. Biol. 236: 217-28, 1994.
108)  Powell BS, Rivas MP, Court DL, Nakamura Y, Turnbough CL.
Rapid confirmation of single copy lambda prophage integration by PCR.
Nucleic Acids Res. 22: 5765-6, 1994.
109)  Patterson TA, Costantino N, Dasgupta S, Court DL.
Improved bacterial hosts for regulated expression of genes from lambda pL plasmid vectors.
Gene. 132: 83-7, 1993.
110)  Oppenheim AB, Kornitzer D, Altuvia S, Court DL.
Posttranscriptional control of the lysogenic pathway in bacteriophage lambda.
Prog. Nucleic Acid Res. Mol. Biol. 46: 37-49, 1993.
111)  Takiff HE, Baker T, Copeland T, Chen SM, Court DL.
Locating essential Escherichia coli genes by using mini-Tn10 transposons: the pdxJ operon.
J. Bacteriol. 174: 1544-53, 1992.
112)  Chen S, Court DL.
Overexpression of rnc gene and purification of RNaseIII.
Chin. J. Biotechnol. 8: 82-91, 1992.
113)  Cheng SW, Lynch EC, Leason KR, Court DL, Shapiro BA, Friedman DI.
Functional importance of sequence in the stem-loop of a transcription terminator.
Science. 254: 1205-7, 1991.
114)  Chen S, Court DL.
High expression of era gene.
Chin. J. Biotechnol. 7: 177-84, 1991.
115)  Kameyama L, Fernandez L, Court DL, Guarneros G.
RNaselll activation of bacteriophage lambda N synthesis.
Mol. Microbiol. 5: 2953-63, 1991.
116)  Chen SM, Takiff HE, Barber AM, Dubois GC, Bardwell JC, Court DL.
Expression and characterization of RNase III and Era proteins. Products of the rnc operon of Escherichia coli.
J. Biol. Chem. 265: 2888-95, 1990.
117)  Marcus-Sekura CJ, Woerner AM, Zweig M, Court DL, Levin JG, Klutch M.
Expression of HIV-1 integrase in E. coli: immunological analysis of the recombinant protein.
AIDS Res. Hum. Retroviruses. 6: 1399-408, 1990.
118)  Guzman P, Rivera Chavira BE, Court DL, Gottesman ME, Guarneros G.
Transcription of a bacteriophage lambda DNA site blocks growth of Escherichia coli.
J. Bacteriol. 172: 1030-4, 1990.
119)  Bardwell JC, Régnier P, Chen SM, Nakamura Y, Grunberg-Manago M, Court DL.
Autoregulation of RNase III operon by mRNA processing.
EMBO J. 8: 3401-7, 1989.
120)  Inada T, Court DL, Ito K, Nakamura Y.
Conditionally lethal amber mutations in the leader peptidase gene of Escherichia coli.
J. Bacteriol. 171: 585-7, 1989.
121)  Takiff HE, Chen SM, Court DL.
Genetic analysis of the rnc operon of Escherichia coli.
J. Bacteriol. 171: 2581-90, 1989.
122)  Nakamura Y, Tsugawa A, Fornwald LW, Showalter SD, Court DL.
Murine monoclonal antibodies which recognize active sites of Escherichia coli NusA protein and epitope mapping by gene fusion.
Gene. 80: 13-9, 1989.
123)  Inada T, Kawakami K, Chen SM, Takiff HE, Court DL, Nakamura Y.
Temperature-sensitive lethal mutant of era, a G protein in Escherichia coli.
J. Bacteriol. 171: 5017-24, 1989.
124)  Levin JG, Crouch RJ, Post K, Hu SC, McKelvin D, Zweig M, Court DL, Gerwin BI.
Functional organization of the murine leukemia virus reverse transcriptase: characterization of a bacterially expressed AKR DNA polymerase deficient in RNase H activity.
J. Virol. 62: 4376-80, 1988.
125)  Tsugawa A, Saito M, Court DL, Nakamura Y.
nusA amber mutation that causes temperature-sensitive growth of Escherichia coli.
J. Bacteriol. 170: 908-15, 1988.
126)  Zuber M, Patterson TA, Court DL.
Analysis of nutR, a site required for transcription antitermination in phage lambda.
Proc. Natl. Acad. Sci. U.S.A. 84: 4514-8, 1987.
127)  Zweig M, Showalter SD, Du Bois GC, Sisk WP, Court DL.
Detection of heterologous fusion proteins in Escherichia coli with a monoclonal antibody.
Gene. 55: 47-53, 1987.
128)  Sisk WP, Chirikjian JG, Lautenberger J, Jorcyk C, Papas TS, Berman ML, Zagursky R, Court DL.
A plasmid vector for cloning and expression of gene segments: expression of an HTLV-I envelope gene segment.
Gene. 48: 183-93, 1986.
129)  Hu SC, Court DL, Zweig M, Levin JG.
Murine leukemia virus pol gene products: analysis with antisera generated against reverse transcriptase and endonuclease fusion proteins expressed in Escherichia coli.
J. Virol. 60: 267-74, 1986.
130)  Montañez C, Bueno J, Schmeissner U, Court DL, Guarneros G.
Mutations of bacteriophage lambda that define independent but overlapping RNA processing and transcription termination sites.
J. Mol. Biol. 191: 29-37, 1986.
131)  Nakamura Y, Mizusawa S, Court DL, Tsugawa A.
Regulatory defects of a conditionally lethal nusAts mutant of Escherichia coli. Positive and negative modulator roles of NusA protein in vivo.
J. Mol. Biol. 189: 103-11, 1986.
132)  Patterson TA, Court DL, Dubuc G, Michniewicz JJ, Goodchild J, Bukhari AI, Narang SA.
Transposition studies of mini-Mu plasmids constructed from the chemically synthesized ends of bacteriophage Mu.
Gene. 50: 101-9, 1986.
133)  Tsugawa A, Kurihara T, Zuber M, Court DL, Nakamura Y.
E. coli NusA protein binds in vitro to an RNA sequence immediately upstream of the boxA signal of bacteriophage lambda.
EMBO J. 4: 2337-42, 1985.
134)  Bear SE, Court DL, Friedman DI.
An accessory role for Escherichia coli integration host factor: characterization of a lambda mutant dependent upon integration host factor for DNA packaging.
J. Virol. 52: 966-72, 1984.
135)  Bear SE, Colberg-Poley AM, Court DL, Carter BJ, Enquist LW.
Analysis of two potential shuttle vectors containing herpes simplex virus defective DNA.
J. Mol. Appl. Genet. 2: 471-84, 1984.
136)  Feiss M, Adyha S, Court DL.
Isolation of plaque-forming, galactose-transducing strains of phage lambda.
Genetics. 71: 189-206, 1972.
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