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Our Science – Randazzo Website

Paul A. Randazzo, M.D., Ph.D.

Selected Publications

1)  Shiba Y, Kametaka S, Waguri S, Presley JF, Randazzo PA.
ArfGAP3 Regulates the Transport of Cation-Independent Mannose 6-Phosphate Receptor in the Post-Golgi Compartment.
Curr. Biol. 23: 1945-51, 2013.
2)  Chen P, Jian X, Yoon H, Randazzo PA.
ARAP2 signals through Arf6 and Rac1 to control focal adhesion morphology.
J. Biol. Chem. 288: 5849-60, 2013.
3)  Hosokawa H, Dip PV, Merkulova M, Bakulina A, Zhuang Z, Khatri A, Jian X, Keating SM, Bueler SA, Rubinstein JL, Randazzo PA, Ausiello DA, Gruber G, Marshansky V.
The N-terminus of a-Subunit Isoforms is Involved in Signaling between V-ATPase and Cytohesin-2.
J. Biol. Chem. 288: 5896-913, 2013.
4)  Chen P, Jian X, Luo R, Randazzo PA.
Approaches to studying Arf GAPs in cells: in vitro assay with isolated focal adhesions.
Curr Protoc Cell Biol. Chapter 17: Unit 17.13, 2012.
5)  Shiba Y, Randazzo PA.
ArfGAP1 function in COPI mediated membrane traffic: currently debated models and comparison to other coat-binding ArfGAPs.
Histol. Histopathol. 27: 1143-53, 2012.
6)  Northup JK, Jian X, Randazzo PA.
Nucleotide exchange factors: Kinetic analyses and the rationale for studying kinetics of GEFs.
Cell Logist. 2: 140-146, 2012.
7)  Luo R, Akpan IO, Hayashi R, Sramko M, Barr V, Shiba Y, Randazzo PA.
The GTP-binding protein-like domain of AGAP1 is a protein binding site that allosterically regulates ArfGAP catalytic activity.
J Biol Chem. 287: 17176-85, 2012.
8)  Jian X, Gruschus JM, Sztul E, Randazzo PA.
The PH domain of the Arf exchange factor Brag2 is an allosteric binding site.
J Biol Chem. 287: 24273-83, 2012.
9)  Yoon HY, Kales SC, Luo R, Lipkowitz S, Randazzo PA.
ARAP1 association with CIN85 affects epidermal growth factor receptor endocytic trafficking.
Biol. Cell. 103: 171-84, 2011.
10)  Shiba Y, Luo R, Hinshaw JE, Szul T, Hayashi R, Sztul E, Nagashima K, Baxa U, Randazzo PA.
ArfGAP1 promotes COPI vesicle formation by facilitating coatomer polymerization.
Cell Logist. 1: 139-154, 2011.
11)  Shiba Y, Randazzo PA.
GEFH1 binds ASAP1 and regulates podosome formation.
Biochem. Biophys. Res. Commun. 406: 574-9, 2011.
12)  Lowery J, Szul T, Seetharaman J, Jian X, Su M, Forouhar F, Xiao R, Acton TB, Montelione GT, Lin H, Wright JW, Lee E, Holloway ZG, Randazzo PA, Tong L, Sztul E.
Novel C-terminal Motif within Sec7 Domain of Guanine Nucleotide Exchange Factors Regulates ADP-ribosylation Factor (ARF) Binding and Activation.
J. Biol. Chem. 286: 36898-906, 2011.
13)  Sakurai A, Jian X, Lee CJ, Manavski Y, Chavakis E, Donaldson J, Randazzo PA, Gutkind JS.
Phosphatidylinositol-4-phosphate 5-kinase and GEP100/Brag2 protein mediate antiangiogenic signaling by semaphorin 3E-plexin-D1 through Arf6 protein.
J. Biol. Chem. 286: 34335-45, 2011.
14)  Chen PW, Randazzo PA, Parent CA.
ACAP-A/B are ArfGAP homologs in dictyostelium involved in sporulation but not in chemotaxis.
PLoS ONE. 5: e8624, 2010.
15)  Spang A, Shiba Y, Randazzo PA.
Arf GAPs: gatekeepers of vesicle generation.
FEBS Lett. 584: 2646-51, 2010.
16)  Jian X, Cavenagh M, Gruschus JM, Randazzo PA, Kahn RA.
Modifications to the C-terminus of Arf1 alter cell functions and protein interactions.
Traffic. 11: 732-42, 2010.
17)  Kang S, Lee E, Yoon H, Randazzo PA, Lee S.
PTK6 inhibits downregulation of EGF receptor through phosphorylation of ARAP1.
J Biol Chem. 285: 26013-21, 2010.
18)  Sakurai A, Gavard J, Annas-Linhares Y, Basile JR, Amornphimoltham P, Palmby TR, Yagi H, Zhang F, Randazzo PA, Li X, Weigert R, Gutkind JS.
Semaphorin 3E initiates antiangiogenic signaling through plexin D1 by regulating Arf6 and R-Ras.
Mol. Cell. Biol. 30: 3086-98, 2010.
19)  Campa F, Yoon H, Ha VL, Szentpetery Z, Balla T, Randazzo PA.
A PH domain in the Arf GAP ARAP1 binds PtdIns(3,4,5)P3 and regulates Arf GAP activity independently of recruitment to the plasma membranes.
J. Biol. Chem. 284: 28069-83, 2009.
20)  Luo R, Ha VL, Hayashi R, Randazzo PA.
Arf GAP2 is positively regulated by coatomer and cargo.
Cell. Signal. 21: 1169-79, 2009.
21)  Jian X, Brown P, Schuck P, Gruschus JM, Balbo A, Hinshaw JE, Randazzo PA.
Autoinhibition of Arf GTPase-activating protein activity by the BAR domain in ASAP1.
J. Biol. Chem. 284: 1652-63, 2009.
22)  Mazelova J, Astuto-Gribble L, Inoue H, Tam BM, Schonteich E, Prekeris R, Moritz OL, Randazzo PA, Deretic D.
Ciliary targeting motif VxPx directs assembly of a trafficking module through Arf4.
EMBO J. 28: 183-92, 2009.
23)  Gruschus JM, Chen PW, Luo R, Randazzo PA.
Journey to the ends of the Arf.
Structure. 17: 2-4, 2009.
24)  Liljegren SJ, Leslie ME, Darnielle L, Lewis MW, Taylor SM, Luo R, Geldner N, Chory J, Randazzo PA, Yanofsky MF, Ecker JR.
Regulation of membrane trafficking and organ separation by the NEVERSHED ARF-GAP protein.
Development. 136: 1909-18, 2009.
25)  Yoon HY, Lee JS, Randazzo PA.
ARAP1 regulates endocytosis of EGFR.
Traffic. 9: 2236-52, 2008.
26)  Inoue H, Ha VL, Prekeris R, Randazzo PA.
Arf GTPase-activating protein ASAP1 interacts with Rab11 effector FIP3 and regulates pericentrosomal localization of transferrin receptor-positive recycling endosome.
Mol. Biol. Cell. 19: 4224-37, 2008.
27)  Campa F, Randazzo PA.
Arf GTPase-activating proteins and their potential role in cell migration and invasion.
Cell Adh Migr. 2: 258-62, 2008.
28)  Ha VL, Bharti S, Inoue H, Vass WC, Campa F, Nie Z, de Gramont A, Ward Y, Randazzo PA.
ASAP3 is a focal adhesion-associated Arf GAP that functions in cell migration and invasion.
J. Biol. Chem. 283: 14915-26, 2008.
29)  Kahn RA, Bruford E, Inoue H, Logsdon JM, Nie Z, Premont RT, Randazzo PA, Satake M, Theibert AB, Zapp ML, Cassel D.
Consensus nomenclature for the human ArfGAP domain-containing proteins.
J. Cell Biol. 182: 1039-44, 2008.
30)  Ha VL, Luo R, Nie Z, Randazzo PA.
Contribution of AZAP-Type Arf GAPs to cancer cell migration and invasion.
Adv. Cancer Res. 101: 1-28, 2008.
31)  Luo R, Miller Jenkins LM, Randazzo PA, Gruschus J.
Dynamic interaction between Arf GAP and PH domains of ASAP1 in the regulation of GAP activity.
Cell Signal. 20: 1968-77, 2008.
32)  Esteban PF, Caprari P, Yoon HY, Randazzo PA, Tessarollo L.
In vitro and in vivo analysis of neurotrophin-3 activation of arf6 and rac-1.
Meth. Enzymol. 438: 171-83, 2008.
33)  Luo R, Randazzo PA.
Kinetic Analysis of Arf GAP1 Indicates a Regulatory Role for Coatomer.
J. Biol. Chem. 283: 21965-77, 2008.
34)  Inoue H, Randazzo PA.
Arf GAPs and Their Interacting Proteins.
Traffic. 8: 1465-75, 2007.
35)  Randazzo PA, Inoue H, Bharti S.
Arf GAPs as regulators of the actin cytoskeleton.
Biol. Cell. 99: 583-600, 2007.
36)  Luo R, Ahvazi B, Amariei D, Shroder D, Burrola B, Losert W, Randazzo PA.
Kinetic analysis of GTP hydrolysis catalysed by the Arf1-GTP-ASAP1 complex.
Biochem. J. 402: 439-47, 2007.
37)  Zhang Q, Major MB, Takanashi S, Camp ND, Nishiya N, Peters EC, Ginsberg MH, Jian X, Randazzo PA, Schultz PG, Moon RT, Ding S.
Small-molecule synergist of the Wnt/beta-catenin signaling pathway.
Proc. Natl. Acad. Sci. U.S.A. 104: 7444-8, 2007.
38)  Bharti S, Inoue H, Bharti K, Hirsch DS, Nie Z, Yoon HY, Artym V, Yamada KM, Mueller SC, Barr VA, Randazzo PA.
Src-dependent phosphorylation of ASAP1 regulates podosomes.
Mol. Cell. Biol. 27: 8271-83, 2007.
39)  Nie Z, Hirsch DS, Luo R, Jian X, Stauffer S, Cremesti A, Andrade J, Lebowitz J, Marino M, Ahvazi B, Hinshaw JE, Randazzo PA.
A BAR domain in the N terminus of the Arf GAP ASAP1 affects membrane structure and trafficking of epidermal growth factor receptor.
Curr. Biol. 16: 130-9, 2006.
40)  Esteban PF, Yoon HY, Becker J, Dorsey SG, Caprari P, Palko ME, Coppola V, Saragovi HU, Randazzo PA, Tessarollo L.
A kinase-deficient TrkC receptor isoform activates Arf6-Rac1 signaling through the scaffold protein tamalin.
J. Cell Biol. 173: 291-9, 2006.
41)  Yoon HY, Miura K, Cuthbert EJ, Davis KK, Ahvazi B, Casanova JE, Randazzo PA.
ARAP2 effects on the actin cytoskeleton are dependent on Arf6-specific GTPase-activating-protein activity and binding to RhoA-GTP.
J. Cell. Sci. 119: 4650-66, 2006.
42)  Nie Z, Randazzo PA.
Arf GAPs and membrane traffic.
J. Cell. Sci. 119: 1203-11, 2006.
43)  Rosenfeldt H, Castellone MD, Randazzo PA, Gutkind JS.
Rac inhibits thrombin-induced Rho activation: evidence of a Pak-dependent GTPase crosstalk.
J Mol Signal. 1: 8, 2006.
44)  Che MM, Nie Z, Randazzo PA.
Assays and properties of the Arf GAPs AGAP1, ASAP1, and Arf GAP1.
Meth. Enzymol. 404: 147-63, 2005.
45)  Yoon HY, Bonifacino JS, Randazzo PA.
In vitro assays of Arf1 interaction with GGA proteins.
Meth. Enzymol. 404: 316-32, 2005.
46)  Luo R, Jacques K, Ahvazi B, Stauffer S, Premont RT, Randazzo PA.
Mutational analysis of the Arf1*GTP/Arf GAP interface reveals an Arf1 mutant that selectively affects the Arf GAP ASAP1.
Curr. Biol. 15: 2164-9, 2005.
47)  Ha VL, Thomas GM, Stauffer S, Randazzo PA.
Preparation of myristoylated Arf1 and Arf6.
Meth. Enzymol. 404: 164-74, 2005.
48)  Che MM, Boja ES, Yoon H, Gruschus J, Jaffe H, Stauffer S, Schuck P, Fales HM, Randazzo PA.
Regulation of ASAP1 by phospholipids is dependent on the interface between the PH and Arf GAP domains.
Cell Signal. 17: 1276-88, 2005.
49)  Nie Z, Fei J, Premont RT, Randazzo PA.
The Arf GAPs AGAP1 and AGAP 2 distinguish between the adaptor protein complexes AP-1 and AP-3.
J Cell Sci. 118: 3555-66, 2005.
50)  I ST, Nie Z, Stewart A, Najdovska M, Hall NE, He H, Randazzo PA, Lock P.
ARAP3 is transiently tyrosine phosphorylated in cells attaching to fibronectin and inhibits cell spreading in a RhoGAP-dependent manner.
J Cell Sci. 117: 6071-84, 2004.
51)  Randazzo PA, Hirsch DS.
Arf GAPs: multifunctional proteins that regulate membrane traffic and actin remodelling.
Cell Signal. 16: 401-13, 2004.
52)  Kowanetz K, Husnjak K, Höller D, Kowanetz M, Soubeyran P, Hirsch D, Schmidt MH, Pavelic K, De Camilli P, Randazzo PA, Dikic I.
CIN85 associates with multiple effectors controlling intracellular trafficking of epidermal growth factor receptors.
Mol Biol Cell. 15: 3155-66, 2004.
53)  Yoon H, Jacques K, Nealon B, Stauffer S, Premont RT, Randazzo PA.
Differences between AGAP1, ASAP1 and ArfGAP1 in substrate recognition: Interaction with the N-terminus of Arf1.
Cell Signal. 16: 1033-1044, 2004.
54)  Nie Z, Hirsch DS, Randazzo PA.
Arf and its many interactors.
Curr Opin Cell Biol. 15: 396-404, 2003.
55)  Randazzo PA, Nie Z, Hirsch DS.
Arf and Phospholipids.
The Arf Book. Editor: Kahn RA, Humana Press, 2003.
56)  Hirsch DS, Stanley KT, Chen LX, Jacques KM, Puertollano R, Randazzo PA.
Arf Regulates Interaction of GGA with Mannose-6-Phosphate Receptor.
Traffic. 4: 26-35, 2003.
57)  Oda A, Wada I, Miura K, Okawa K, Kadoya T, Kato T, Nishihara H, Maeda M, Tanaka S, Nagashima K, Nishitani C, Matsuno K, Ishino M, Machesky LM, Fujita H, Randazzo P.
CrkL directs ASAP1 to peripheral focal adhesions.
J Biol Chem. 278: 6456-60, 2003.
58)  Randazzo PA.
RhoD, Src, and hDia2C in endosome motility.
Dev Cell. 4: 287-8, 2003.
59)  Nie Z, Boehm M, Boja ES, Vass WC, Bonifacino JS, Fales HM, Randazzo PA.
Specific regulation of the adaptor protein complex AP-3 by the Arf GAP AGAP1.
Dev Cell. 5: 513-21, 2003.
60)  Yanagisawa LL, Marchena J, Xie Z, Li X, Poon PP, Singer RA, Johnston GC, Randazzo PA, Bankaitis VA.
Activity of specific lipid-regulated ADP ribosylation factor-GTPase-activating proteins is required for Sec14p-dependent Golgi secretory function in yeast.
Mol Biol Cell. 13: 2193-206, 2002.
61)  Nie Z, Stanley KT, Stauffer S, Jacques KM, Hirsch DS, Takei J, Randazzo PA.
AGAP1, an Endosome-associated, Phosphoinositide-dependent ADP-ribosylation Factor GTPase-activating Protein That Affects Actin Cytoskeleton.
J Biol Chem. 277: 48965-75, 2002.
62)  Miura K, Jacques KM, Stauffer S, Kubosaki A, Zhu K, Hirsch DS, Resau J, Zheng Y, Randazzo PA.
ARAP1: a point of convergence for Arf and Rho signaling.
Mol Cell. 9: 109-19, 2002.
63)  Jacques KM, Nie Z, Stauffer S, Hirsch DS, Chen LX, Stanley KT, Randazzo PA.
Arf1 Dissociates from the Clathrin Adaptor GGA Prior to Being Inactivated by Arf GTPase-activating Proteins.
J Biol Chem. 277: 47235-41, 2002.
64)  Yang JS, Lee SY, Gao M, Bourgoin S, Randazzo PA, Premont RT, Hsu VW.
ARFGAP1 promotes the formation of COPI vesicles, suggesting function as a component of the coat.
J Cell Biol. 159: 69-78, 2002.
65)  Randazzo PA, Fales HM.
Preparation of myristoylated Arf1 and Arf6 proteins.
Methods Mol Biol. 189: 169-79, 2002.
66)  Puertollano R, Randazzo PA, Presley JF, Hartnell LM, Bonifacino JS.
The GGAs promote ARF-dependent recruitment of clathrin to the TGN.
Cell. 105: 93-102, 2001.
67)  Jackson TR, Brown FD, Nie Z, Miura K, Foroni L, Sun J, Hsu VW, Donaldson JG, Randazzo PA.
ACAPs are arf6 GTPase-activating proteins that function in the cell periphery.
J Cell Biol. 151: 627-38, 2000.
68)  Randazzo PA, Nie Z, Miura K, Hsu VW.
Molecular aspects of the cellular activities of ADP-ribosylation factors.
Sci STKE. 2000: RE1, 2000.
69)  Kam JL, Miura K, Jackson TR, Gruschus J, Roller P, Stauffer S, Clark J, Aneja R, Randazzo PA.
Phosphoinositide-dependent activation of the ADP-ribosylation factor GTPase-activating protein ASAP1. Evidence for the pleckstrin homology domain functioning as an allosteric site.
J Biol Chem. 275: 9653-63, 2000.
70)  Randazzo PA, Andrade J, Miura K, Brown MT, Long YQ, Stauffer S, Roller P, Cooper JA.
The Arf GTPase-activating protein ASAP1 regulates the actin cytoskeleton.
Proc Natl Acad Sci U S A. 97: 4011-6, 2000.
71)  Andreev J, Simon JP, Sabatini DD, Kam J, Plowman G, Randazzo PA, Schlessinger J.
Identification of a new Pyk2 target protein with Arf-GAP activity.
Mol Cell Biol. 19: 2338-50, 1999.
72)  Brown MT, Andrade J, Radhakrishna H, Donaldson JG, Cooper JA, Randazzo PA.
ASAP1, a phospholipid-dependent arf GTPase-activating protein that associates with and is phosphorylated by Src.
Mol Cell Biol. 18: 7038-51, 1998.
73)  Randazzo PA.
Functional interaction of ADP-ribosylation factor 1 with phosphatidylinositol 4,5-bisphosphate.
J Biol Chem. 272: 7688-92, 1997.
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This page was last updated on 11/13/2013.