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Shyam K. Sharan, Ph.D.

Selected Publications

1)  Bakker JL, Thirthagiri E, van Mil SE, Adank MA, Ikeda H, Verheul HM, Meijers-Heijboer H, de Winter JP, Sharan SK, Waisfisz Q.
A Novel Splice Site Mutation in the Non-coding Region of BRCA2: Implications for Fanconi Anemia and Familial Breast Cancer Diagnostics.
Hum. Mutat. 2014.
2)  Mukherjee M, Ge G, Zhang N, Edwards DG, Sumazin P, Sharan SK, Rao PH, Medina D, Pati D.
MMTV-Espl1 transgenic mice develop aneuploid, estrogen receptor alpha (ERa)-positive mammary adenocarcinomas.
Oncogene. 2013.
3)  Chang S, Sharan SK.
The role of epigenetic transcriptional regulation in BRCA1-mediated tumor suppression.
Transcription. Jan-Feb;4(1): 24-28, 2013.
Full Text Article. [Journal]
4)  Chang S, Sharan SK.
BRCA1 and MicroRNAs: Emerging networks and potential therapeutic targets.
Mol. Cells. 34: 425-32, 2012.
5)  Biswas K, Das R, Eggington JM, Qiao H, North SL, Stauffer S, Burkett SS, Martin BK, Southon E, Sizemore SC, Pruss D, Bowles KR, Roa BB, Hunter N, Tessarollo L, Wenstrup RJ, Byrd RA, Sharan SK.
Functional evaluation of BRCA2 variants mapping to the PALB2-binding and C-terminal DNA-binding domains using a mouse ES cell-based assay.
Hum. Mol. Genet. 21: 3993-4006, 2012.
6)  Chang S, Wang RH, Akagi K, Kim KA, Martin BK, Cavallone L, Haines DC, Basik M, Mai P, Poggi E, Isaacs C, Looi LM, Mun KS, Greene MH, Byers SW, Teo SH, Deng CX, Sharan SK.
Tumor suppressor BRCA1 epigenetically controls oncogenic microRNA-155.
Nat. Med. 17: 1275-82, 2011.
7)  Biswas K, Das R, Alter BP, Kuznetsov SG, Stauffer S, North SL, Burkett S, Brody LC, Meyer S, Byrd RA, Sharan SK.
A comprehensive functional characterization of BRCA2 variants associated with Fanconi anemia using mouse ES cell-based assay.
Blood. 118: 2430-42, 2011.
8)  Chang S, Biswas K, Martin BK, Stauffer S, Sharan SK.
Expression of human BRCA1 variants in mouse ES cells allows functional analysis of BRCA1 mutations.
J. Clin. Invest. 119: 3160-71, 2009.
9)  Sharan SK, Thomason LC, Kuznetsov SG, Court DL.
Recombineering: a homologous recombination-based method of genetic engineering.
Nat Protoc. 4: 206-23, 2009.
10)  Kuznetsov SG, Liu P, Sharan SK.
Mouse embryonic stem cell-based functional assay to evaluate mutations in BRCA2.
Nat. Med. 14: 875-81, 2008.
11)  Philip S, Swaminathan S, Kuznetsov SG, Kanugula S, Biswas K, Chang S, Loktionova NA, Haines DC, Kaldis P, Pegg AE, Sharan SK.
Degradation of BRCA2 in Alkyltransferase-Mediated DNA Repair and Its Clinical Implications.
Cancer Res. 68: 9973-81, 2008.
12)  Biswas K, Sharan SK.
Manipulating the mouse genome using recombineering.
Advancements in Genetic Engineering. 4: 1-5, 2013.
13)  Chang S, Sharan SK.
Epigenetic control of an oncogenic microRNA, miR-155, by BRCA1.
Oncotarget. 3: 5-6, 2012.
14)  Chang S, Stauffer S, Sharan SK.
Using recombineering to generate point mutations: the oligonucleotide-based.
Methods Mol. Biol. 852: 111-20, 2012.
15)  Biswas K, Stauffer S, Sharan SK.
Using recombineering to generate point mutations:galK-based positive-negative selection method.
Methods Mol. Biol. 852: 121-31, 2012.
16)  Pellegrini M, Claps G, Orlova VV, Barrios F, Dolci S, Geremia R, Rossi P, Rossi G, Arnold B, Chavakis T, Feigenbaum L, Sharan SK, Nussenzweig A.
Targeted JAM-C deletion in germ cells by Spo11-controlled Cre recombinase.
J. Cell. Sci. 124: 91-9, 2011.
17)  Kuznetsov SG, Chang S, Sharan SK.
Functional analysis of human BRCA2 variants using a mouse embryonic stem cell-based assay.
Methods Mol. Biol. 653: 259-80, 2010.
18)  Kuznetsov S, Pellegrini M, Shuda K, Fernandez-Capetillo O, Liu Y, Martin BK, Burkett S, Southon E, Pati D, Tessarollo L, West SC, Donovan PJ, Nussenzweig A, Sharan SK.
RAD51C deficiency in mice results in early prophase I arrest in males and sister chromatid separation at metaphase II in females.
J. Cell Biol. 176: 581-92, 2007.
19)  Li L, Biswas K, Habib LA, Kuznetsov SG, Hamel N, Kirchhoff T, Wong N, Armel S, Chong G, Narod SA, Claes K, Offit K, Robson ME, Stauffer S, Sharan SK, Foulkes WD.
Functional redundancy of exon 12 of BRCA2 revealed by a comprehensive analysis of the c.6853A>G (p.I2285V) variant.
Hum. Mutat. 30: 1543-50, 2009.
20)  Kuznetsov SG, Haines DC, Martin BK, Sharan SK.
Loss of rad51c leads to embryonic lethality and modulation of Trp53-dependent tumorigenesis in mice.
Cancer Res. 69: 863-72, 2009.
21)  Zhang N, Kuznetsov SG, Sharan SK, Li K, Rao PH, Pati D.
A handcuff model for the cohesin complex.
J. Cell Biol. 183: 1019-31, 2008.
22)  Posey KL, Yang Y, Veerisetty AC, Sharan SK, Hecht JT.
Model systems for studying skeletal dysplasias caused by TSP-5/COMP mutations.
Cell. Mol. Life Sci. 65: 687-99, 2008.
23)  Sharan SK, Kuznetsov S.
Resolving RAD51C function in late stages of homologous recombination.
Cell Div. 2: 15, 2007.
24)  Senatorov V, Malyukova I, Fariss R, Wawrousek EF, Swaminathan S, Sharan SK, Tomarev S.
Expression of mutated mouse myocilin induces open-angle glaucoma in transgenic mice.
J. Neurosci. 26: 11903-14, 2006.
25)  Difilippantonio S, Celeste A, Fernandez-Capetillo O, Chen HT, Reina San Martin B, Van Laethem F, Yang YP, Petukhova GV, Eckhaus M, Feigenbaum L, Manova K, Kruhlak M, Camerini-Otero RD, Sharan S, Nussenzweig M, Nussenzweig A.
Role of Nbs1 in the activation of the Atm kinase revealed in humanized mouse models.
Nat. Cell Biol. 7: 675-85, 2005.
26)  Sharan SK, Pyle A, Coppola V, Babus J, Swaminathan S, Benedict J, Swing D, Martin BK, Tessarollo L, Evans JP, Flaws JA, Handel MA.
BRCA2 Deficiency in mice leads to meiotic impairment and infertility.
Development. 131: 131-142, 2004.
27)  Swaminathan S, Sharan SK.
Bacterial artificial chromosome engineering.
Methods Mol Biol. 256: 89-106, 2004.
28)  Swing DA, Sharan SK.
BAC Rescue: A Tool for Functional Analysis of the Mouse Genome.
Methods Mol Biol. 256: 183-98, 2004.
29)  Yang Y, Swaminathan S, Martin BK, Sharan SK.
Aberrant splicing induced by missense mutations in BRCA1: clues from a humanized mouse model.
Hum Mol Genet. 12: 2121-31, 2003.
30)  Yang Y, Sharan SK.
A simple two-step, 'hit and fix' method to generate subtle mutations in BACs using short denatured PCR fragments.
Nucleic Acids Res. 31: e80, 2003.
31)  Court DL, Swaminathan S, Yu D, Wilson H, Baker T, Bubunenko M, Sawitzke J, Sharan SK.
Mini-lambda: a tractable system for chromosome and BAC engineering.
Gene. 315: 63-9, 2003.
32)  Kraakman-van der Zwet M, Overkamp WJ, van Lange RE, Essers J, van Duijn-Goedhart A, Wiggers I, Swaminathan S, van Buul PP, Errami A, Tan RT, Jaspers NG, Sharan SK, Kanaar R, Zdzienicka MZ.
Brca2 (XRCC11) deficiency results in radioresistant DNA synthesis and a higher frequency of spontaneous deletions.
Mol. Cell. Biol. 22: 669-79, 2002.
33)  Leasure CS, Chandler J, Gilbert DJ, Householder DB, Stephens R, Copeland NG, Jenkins NA, Sharan SK.
Sequence, chromosomal location and expression analysis of the murine homologue of human RAD51L2/RAD51C.
Gene. 271: 59-67, 2001.
34)  Chandler J, Hohenstein P, Swing DA, Tessarollo L, Sharan SK.
Human BRCA1 gene rescues the embryonic lethality of Brca1 mutant mice.
Genesis. 29: 72-7, 2001.
35)  Swaminathan S, Ellis HM, Waters LS, Yu D, Lee EC, Court DL, Sharan SK.
Rapid engineering of bacterial artificial chromosomes using oligonucleotides.
Genesis. 29: 14-21, 2001.
36)  Sharan SK, Bradley A.
Functional characterization of BRCA1 and BRCA2: clues from their interacting proteins.
J. Mammary Gland Biol. Neoplasia. 3: 413-21, 1998.
37)  Sharan SK, Morimatsu M, Albrecht U, Lim DS, Regel E, Dinh C, Sands A, Eichele G, Hasty P, Bradley A.
Embryonic lethality and radiation hypersensitivity mediated by Rad51 in mice lacking Brca2.
Nature. 386: 804-10, 1997.
38)  Sharan SK, Bradley A.
Murine Brca2: sequence, map position, and expression pattern.
Genomics. 40: 234-41, 1997.
39)  Sharan SK, Bradley A.
Identification and characterization of a microsatellite marker within murine Brca2 gene.
Mamm. Genome. 8: 79, 1997.
40)  Bradley A, Sharan SK.
... and secreted tumour suppressors.
Nat. Genet. 13: 268-9; author reply 269-72, 1996.
41)  Sharan SK, Wims M, Bradley A.
Murine Brca1: sequence and significance for human missense mutations.
Hum. Mol. Genet. 4: 2275-8, 1995.
42)  Holdener BC, Thomas JW, Schumacher A, Potter MD, Rinchik EM, Sharan SK, Magnuson T.
Physical localization of eed: a region of mouse chromosome 7 required for gastrulation.
Genomics. 27: 447-56, 1995.
43)  Sharan SK, Bradley A.
Role of transgenic mice in identification and characterization of tumour suppressor genes.
Cancer Surv. 25: 143-59, 1995.
44)  Sharan SK, Holdener-Kenny B, Threadgill DW, Magnuson T.
Genomic mapping within the albino-deletion complex using individual early postimplantation mouse embryos.
Mamm. Genome. 3: 79-83, 1992.
45)  Holdener-Kenny B, Sharan SK, Magnuson T.
Mouse albino-deletions: from genetics to genes in development.
Bioessays. 14: 831-9, 1992.
46)  Sharan SK, Holdener-Kenny B, Ruppert S, Schedl A, Kelsey G, Rinchik EM, Magnuson T.
The albino-deletion complex of the mouse: molecular mapping of deletion breakpoints that define regions necessary for development of the embryonic and extraembryonic ectoderm.
Genetics. 129: 825-32, 1991.
47)  Niswander L, Kelsey G, Schedl A, Ruppert S, Sharan SK, Holdener-Kenny B, Rinchik EM, Edström JE, Magnuson T.
Molecular mapping of albino deletions associated with early embryonic lethality in the mouse.
Genomics. 9: 162-9, 1991.
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This page was last updated on 1/13/2014.