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Wei-Shau Hu, Ph.D.

Selected Publications

1)  Nikolaitchik O, Dilley K, Fu W, Gorelick R, Tai S, Soheilian F, Ptak R, Nagashima K, Pathak V, Hu W.
Dimeric RNA recognition regulates HIV-1 genome packaging.
PLoS Pathog. 9(3): e1003249, 2013.
Full Text Article. [Journal]
2)  Moore MD, Nikolaitchik OA, Chen J, Hammarskjöld ML, Rekosh D, Hu WS.
Probing the HIV-1 genomic RNA trafficking pathway and dimerization by genetic recombination and single virion analyses.
PLoS Pathog. 5: e1000627, 2009.
3)  Chen J, Nikolaitchik O, Singh J, Wright A, Bencsics CE, Coffin JM, Ni N, Lockett S, Pathak VK, Hu WS.
High efficiency of HIV-1 genomic RNA packaging and heterozygote formation revealed by single virion analysis.
Proc. Natl. Acad. Sci. U.S.A. 106: 13535-40, 2009.
4)  Boyko V, Leavitt M, Gorelick R, Fu W, Nikolaitchik O, Pathak VK, Nagashima K, Hu WS.
Coassembly and Complementation of Gag Proteins from HIV-1 and HIV-2, Two Distinct Human Pathogens.
Mol. Cell. 23: 281-7, 2006.
5)  Chin MP, Rhodes TD, Chen J, Fu W, Hu WS.
Identification of a major restriction in HIV-1 intersubtype recombination.
Proc Natl Acad Sci U S A. 102: 9002-9007, 2005.
6)  Kuzembayeva M, Dilley K, Sardo L, Hu W.
Life of Psi: How full-length HIV-1 RNAs become packaged genomes in the viral particles.
Virology. 2014.
In Press. [Journal]
7)  Nikolaitchik O, Hu W.
Deciphering the role of the Gag-Pol ribosomal frameshift signal in HIV-1 RNA genome packaging.
J Virol. 88: 4040-6, 2014.
8)  Burdick R, Hu W, Pathak V.
Nuclear import of APOBEC3F-labeled HIV-1 preintegration complexes.
Proc Natl Acad Sci USA Plus. 110: E4780-9, 2013.
9)  Chen J, Nikolaitchik O, Dilley K, Hu W.
Freed E, eds.
Packaging of the HIV-1 RNA genome. In: Advances in HIV-1 Assembly and Release.
Switzerland: Springer International Publishing; 2013. p. 55-73 [Book Chapter]
10)  Chaipan C, Smith JL, Hu WS, Pathak VK.
APOBEC3G Restricts HIV-1 to a Greater Extent than APOBEC3F and APOBEC3DE in Human Primary CD4+ T Cells and Macrophages.
J. Virol. 87: 444-53, 2013.
11)  Hatch SC, Sardo L, Chen J, Burdick R, Gorelick R, Fivash MJ, Pathak VK, Hu WS.
Gag-Dependent Enrichment of HIV-1 RNA near the Uropod Membrane of Polarized T Cells.
J. Virol. 87: 11912-11915, 2013.
12)  Delviks-Frankenberry K, Paprotka T, Cingöz O, Wildt S, Hu WS, Coffin JM, Pathak VK.
Generation of Multiple Replication-Competent Retroviruses through Recombination between PreXMRV-1 and PreXMRV-2.
J. Virol. 87: 11525-11537, 2013.
13)  Izumi T, Burdick R, Shigemi M, Plisov S, Hu WS, Pathak VK.
Mov10 and APOBEC3G Localization to Processing Bodies Is Not Required for Virion Incorporation and Antiviral Activity.
J. Virol. 87: 11047-62, 2013.
14)  Cingöz O, Paprotka T, Delviks-Frankenberry KA, Wildt S, Hu WS, Pathak VK, Coffin JM.
Characterization, Mapping, and Distribution of the Two XMRV Parental Proviruses.
J. Virol. 86: 328-38, 2012.
15)  Hu WS, Hughes SH.
HIV-1 Reverse Transcription.
Cold Spring Harb Perspect Med. 2, 2012.
16)  Dilley KA, Ni N, Nikolaitchik OA, Chen J, Galli A, Hu WS.
Determining the frequency and mechanisms of HIV-1 and HIV-2 RNA copackaging by single-virion analysis.
J. Virol. 85: 10499-508, 2011.
17)  Josefsson L, King MS, Makitalo B, Brännström J, Shao W, Maldarelli F, Kearney MF, Hu WS, Chen J, Gaines H, Mellors JW, Albert J, Coffin JM, Palmer SE.
Majority of CD4+ T cells from peripheral blood of HIV-1-infected individuals contain only one HIV DNA molecule.
Proc. Natl. Acad. Sci. U.S.A. 108: 11199-204, 2011.
18)  Delviks-Frankenberry K, Galli A, Nikolaitchik O, Mens H, Pathak VK, Hu WS.
Mechanisms and Factors that Influence High Frequency Retroviral Recombination.
Viruses. 3: 1650-80, 2011.
19)  Ni N, Nikolaitchik OA, Dilley KA, Chen J, Galli A, Fu W, Prasad VV, Ptak RG, Pathak VK, Hu WS.
Mechanisms of human immunodeficiency virus type 2 RNA packaging: efficient trans packaging and selection of RNA copackaging partners.
J. Virol. 85: 7603-12, 2011.
20)  Nikolaitchik OA, Galli A, Moore MD, Pathak VK, Hu WS.
Multiple Barriers to Recombination between Divergent HIV-1 Variants Revealed by a Dual-Marker Recombination Assay.
J. Mol. Biol. 407: 521-31, 2011.
21)  Paprotka T, Delviks-Frankenberry K, Cingoz O, Martinez A, Kung H, Tepper C, Hu W, Fivash M, Coffin J, Pathak V.
Recombinant origin of the retrovirus XMRV.
Science. 333: 97-101, 2011.
Full Text Article. [Journal]
22)  Chaipan C, Dilley KA, Paprotka T, Delviks-Frankenberry KA, Venkatachari NJ, Hu WS, Pathak VK.
Severe Restriction of Xenotropic Murine Leukemia Virus-Related Virus Replication and Spread in Cultured Human Peripheral Blood Mononuclear Cells.
Journal of virology. 85: 4888-4897, 2011.
23)  Hussein IT, Ni N, Galli A, Chen J, Moore MD, Hu WS.
Delineation of the preferences and requirements of the human immunodeficiency virus type 1 dimerization initiation signal by using an in vivo cell-based selection approach.
J. Virol. 84: 6866-75, 2010.
24)  Paprotka T, Venkatachari NJ, Chaipan C, Burdick R, Delviks-Frankenberry KA, Hu WS, Pathak VK.
Inhibition of xenotropic murine leukemia virus-related virus by APOBEC3 proteins and antiviral drugs.
J. Virol. 84: 5719-29, 2010.
25)  Burdick R, Smith JL, Chaipan C, Friew Y, Chen J, Venkatachari NJ, Delviks-Frankenberry KA, Hu WS, Pathak VK.
P body-associated protein Mov10 inhibits HIV-1 replication at multiple stages.
J. Virol. 84: 10241-53, 2010.
26)  Galli A, Kearney M, Nikolaitchik OA, Yu S, Chin MP, Maldarelli F, Coffin JM, Pathak VK, Hu WS.
Patterns of Human Immunodeficiency Virus type 1 recombination ex vivo provide evidence for coadaptation of distant sites, resulting in purifying selection for intersubtype recombinants during replication.
J. Virol. 84: 7651-61, 2010.
27)  Russell R, Moore M, Hu W, Pathak V.
APOBEC3G induces a hypermutation gradient: Purifying selection at multiple steps during HIV-1 replication results in levels of G-to-A mutations that are high in DNA, intermediate in cellular viral RNA, and low in virion RNA.
Retrovirology. 6: 16, 2009.
Full Text Article. [Journal]
28)  Moore MD, Chin MP, Hu WS.
HIV-1 Recombination: An Experimental Assay and a Phylogenetic Approach.
Methods Mol. Biol. 485: 87-105, 2009.
29)  Moore MD, Hu WS.
HIV-1 RNA Dimerization: It Takes Two to Tango.
AIDS reviews. 11: 91-102, 2009.
30)  Friew YN, Boyko V, Hu WS, Pathak VK.
Intracellular interactions between APOBEC3G, RNA, and HIV-1 Gag: APOBEC3G multimerization is dependent on its association with RNA.
Retrovirology. 6: 56, 2009.
31)  Chen J, Pathak VK, Peng W, Hu W.
Capsid proteins from human immunodeficiency virus type 1 and simian immunodeficiency virus SIVmac can coassemble into mature cores of infectious viruses.
J. Virol. 82: 8253-61, 2008.
32)  Moore MD, Fu W, Soheilian F, Nagashima K, Ptak RG, Pathak VK, Hu W.
Suboptimal inhibition of protease activity in human immunodeficiency virus type 1: Effects on virion morphogenesis and RNA maturation.
Virology. 379: 152-60, 2008.
33)  Nikolaitchik OA, Gorelick RJ, Leavitt MG, Pathak VK, Hu W.
Functional complementation of nucleocapsid and late domain PTAP mutants of human immunodeficiency virus type 1 during replication.
Virology. 375: 539-49, 2008.
34)  Motomura K, Chen J, Hu WS.
Genetic recombination between human immunodeficiency virus type 1 (HIV-1) and HIV-2, two distinct human lentiviruses.
J. Virol. 82: 1923-33, 2008.
35)  Chukkapalli V, Hogue IB, Boyko V, Hu WS, Ono A.
Interaction between the human immunodeficiency virus type 1 Gag matrix domain and phosphatidylinositol-(4,5)-bisphosphate is essential for efficient gag membrane binding.
J. Virol. 82: 2405-17, 2008.
36)  Chin MP, Lee SK, Chen J, Nikolaitchik OA, Powell DA, Fivash MJ, Hu WS.
Long-range recombination gradient between HIV-1 subtypes B and C variants caused by sequence differences in the dimerization initiation signal region.
J. Mol. Biol. 377: 1324-33, 2008.
37)  Lee SK, Boyko V, Hu WS.
Capsid is an important determinant for functional complementation of murine leukemia virus and spleen necrosis virus Gag proteins.
Virology. 360: 388-97, 2007.
38)  Thomas DC, Voronin YA, Nikolenko GN, Chen J, Hu WS, Pathak VK.
Determination of the ex vivo rates of human immunodeficiency virus type 1 reverse transcription by using novel strand-specific amplification analysis.
J. Virol. 81: 4798-807, 2007.
39)  Moore MD, Fu W, Nikolaitchik O, Chen J, Ptak RG, Hu WS.
Dimer initiation signal of human immunodeficiency virus type 1: its role in partner selection during RNA copackaging and its effects on recombination.
J. Virol. 81: 4002-11, 2007.
40)  Chin MP, Chen J, Nikolaitchik OA, Hu WS.
Molecular determinants of HIV-1 intersubtype recombination potential.
Virology. 363: 437-46, 2007.
41)  Fu W, Prasad VV, Chen J, Nikolaitchik O, Hu WS.
Molecular mechanisms of simian immunodeficiency virus SIV(agm) RNA encapsidation.
Virology. 363: 210-9, 2007.
42)  Xu H, Chertova E, Chen J, Ott DE, Roser JD, Hu WS, Pathak VK.
Stoichiometry of the antiviral protein APOBEC3G in HIV-1 virions.
Virology. 360: 247-56, 2007.
43)  Fu W, Dang Q, Nagashima K, Freed EO, Pathak VK, Hu WS.
Effects of Gag mutation and processing on retroviral dimeric RNA maturation.
J Virol. 80: 1242-9, 2006.
44)  Nikolaitchik O, Rhodes TD, Ott D, Hu WS.
Effects of mutations in the human immunodeficiency virus type 1 Gag gene on RNA packaging and recombination.
J Virol. 80: 4691-7, 2006.
45)  Chen J, Powell D, Hu WS.
High frequency of genetic recombination is a common feature of primate lentivirus replication.
J. Virol. 80: 9651-8, 2006.
46)  Chen J, Rhodes TD, Hu WS.
Comparison of the genetic recombination rates of human immunodeficiency virus type 1 in macrophages and T cells.
J Virol. 79: 9337-40, 2005.
47)  Lee SK, Nagashima K, Hu WS.
Cooperative effect of Gag proteins p12 and capsid during early events of murine leukemia virus replication.
J Virol. 79: 4159-69, 2005.
48)  Rhodes TD, Nikolaitchik O, Chen J, Powell D, Hu WS.
Genetic recombination of human immunodeficiency virus type 1 in one round of viral replication: Effects of genetic distance, target cells, accessory genes, and lack of high negative interference in crossover events.
J Virol. 79: 1666-77, 2005.
49)  Chen J, Dang Q, Unutmaz D, Pathak VK, Maldarelli F, Powell D, Hu WS.
Mechanisms of nonrandom human immunodeficiency virus type 1 infection and double infection: preference in virus entry is important but is not the sole factor.
J Virol. 79: 4140-9, 2005.
50)  Svarovskaia ES, Xu H, Mbisa JL, Barr R, Gorelick RJ, Ono A, Freed EO, Hu WS, Pathak VK.
Human apolipoprotein B mRNA-editing enzyme-catalytic polypeptide-like 3G (APOBEC3G) is incorporated into HIV-1 virions through interactions with viral and nonviral RNAs.
J Biol Chem. 279: 35822-8, 2004.
51)  Dang Q, Chen J, Unutmaz D, Coffin JM, Pathak VK, Powell D, KewalRamani VN, Maldarelli F, Hu WS.
Nonrandom HIV-1 infection and double infection via direct and cell-mediated pathways.
Proc Natl Acad Sci U S A. 101: 632-7, 2004.
52)  Cheslock SR, Poon DT, Fu W, Rhodes TD, Henderson LE, Nagashima K, McGrath CF, Hu WS.
Charged assembly helix motif in murine leukemia virus capsid: an important region for virus assembly and particle size determination.
J Virol. 77: 7058-66, 2003.
53)  Cheslock SR, Anderson JA, Hwang CK, Pathak VK, Hu WS.
Utilization of nonviral sequences for minus-strand DNA transfer and gene reconstitution during retroviral replication.
J Virol. 74: 9571-9, 2000.
54)  Fu W, Hu WS.
Functional replacement of nucleocapsid flanking regions by heterologous counterparts with divergent primary sequences: effects of chimeric nucleocapsid on the retroviral replication cycle.
J Virol. 77: 754-61, 2003.
55)  Rhodes T, Wargo H, Hu WS.
High rates of human immunodeficiency virus type 1 recombination: near-random segregation of markers one kilobase apart in one round of viral replication.
J Virol. 77: 11193-200, 2003.
56)  Svarovskaia ES, Cheslock SR, Zhang WH, Hu WS, Pathak VK.
Retroviral mutation rates and reverse transcriptase fidelity.
Front Biosci. 8: D117-34, 2003.
57)  Hu WS, Rhodes T, Dang Q, Pathak V.
Retroviral recombination: review of genetic analyses.
Front Biosci. 8: D143-55, 2003.
58)  Beasley BE, Hu WS.
cis-Acting elements important for retroviral RNA packaging specificity.
J Virol. 76: 4950-60, 2002.
59)  Zhang WH, Hwang CK, Hu WS, Gorelick RJ, Pathak VK.
Zinc finger domain of murine leukemia virus nucleocapsid protein enhances the rate of viral DNA synthesis in vivo.
J Virol. 76: 7473-84, 2002.
60)  Dang Q, Hu WS.
Effects of homology length in the repeat region on minus-strand DNA transfer and retroviral replication.
J Virol. 75: 809-20, 2001.
61)  Hu WS, Pathak VK.
Design of retroviral vectors and helper cells for gene therapy.
Pharmacol Rev. 52: 493-511, 2000.
62)  Anderson JA, Pathak VK, Hu WS.
Effect of the murine leukemia virus extended packaging signal on the rates and locations of retroviral recombination.
J Virol. 74: 6953-63, 2000.
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This page was last updated on 4/3/2014.