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Our Science – Le Grice Website

Stuart F.J. Le Grice, Ph.D.

Selected Publications

1)  Costi R, Métifiot M, Chung S, Cuzzucoli Crucitti G, Maddali K, Pescatori L, Messore A, Madia V, Pupo G, Scipione L, Tortorella S, Di Leva F, Cosconati S, Marinelli L, Novellino E, Le Grice S, Corona A, Pommier Y, Marchand C, Di Santo R.
Basic quinolinonyl diketo acid derivatives as inhibitors of HIV integrase and their activity against RNase H function of reverse transcriptase.
J Med Chem. 2014.
In Press. [Journal]
2)  Nowak E, Miller J, Bona M, Studnicka J, Szczepanowski R, Jurkowski J, Le Grice S, Nowotny M.
Ty3 reverse transcriptase complexed with an RNA-DNA hybrid shows structural and functional asymmetry.
Nat. Struct. Mol. Biol. 21: 389-96, 2014.
3)  Abbondanzieri E, Le Grice S.
Unraveling the gymnastics of reverse transcription through single molecule spectroscopy.
AIDS Res Hum Retroviruses. 30: 209-210, 2014.
Full Text Article. [Journal]
4)  Fang X, Wang J, O'Carroll IP, Mitchell M, Zuo X, Wang Y, Yu P, Liu Y, Rausch JW, Dyba MA, Kjems J, Schwieters CD, Seifert S, Winans RE, Watts NR, Stahl SJ, Wingfield PT, Byrd RA, Le Grice SF, Rein A, Wang Y.
An Unusual Topological Structure of the HIV-1 Rev Response Element.
Cell. 155: 594-605, 2013.
Full Text Article. [Journal]
5)  Lusvarghi S, Sztuba-Solinska J, Purzycka KJ, Pauly GT, Rausch JW, Grice SF.
The HIV-2 Rev-response element: determining secondary structure and defining folding intermediates.
Nucleic Acids Res. 41: 6637-49, 2013.
6)  Sztuba-Solinska J, Teramoto T, Rausch JW, Shapiro BA, Padmanabhan R, Le Grice SF.
Structural complexity of Dengue virus untranslated regions: cis-acting RNA motifs and pseudoknot interactions modulating functionality of the viral genome.
Nucleic Acids Res. 41: 5075-89, 2013.
Full Text Article. [Journal]
7)  Lapkouski M, Tian L, Miller JT, Le Grice SF, Yang W.
Complexes of HIV-1 RT, NNRTI and RNA/DNA hybrid reveal a structure compatible with RNA degradation.
Nat. Struct. Mol. Biol. 20: 230-6, 2013.
8)  Chamanian M, Purzycka KJ, Wille PT, Ha JS, McDonald D, Gao Y, Le Grice SF, Arts EJ.
A cis-Acting Element in Retroviral Genomic RNA Links Gag-Pol Ribosomal Frameshifting to Selective Viral RNA Encapsidation.
Cell Host Microbe. 13: 181-92, 2013.
9)  Lapkouski M, Tian L, Miller J, Le Grice S, Yang W.
Reply to “Structural requirements for RNA degradation by HIV-1 reverse transcriptase”.
Nat Struct Mol Biol. 20: 1342-1343, 2013.
Full Text Article. [Journal]
10)  Corona A, Masaoka T, Tocco G, Tramontano E, Le Grice SF.
Active site and allosteric inhibitors of the ribonuclease H activity of HIV reverse transcriptase.
Future Med Chem. 5: 2127-39, 2013.
11)  Le Grice S, Nowotny M.
Reverse transcriptases. In: Nucleic Acid Polymerases (K. Murakami and M. Takselis, eds.).
New York: Springer Publishing; 2014. p. 189-214 [Book Chapter]
12)  Sztuba-Solinska J, Le Grice S.
Insights into secondary and tertiary interactions of dengue virus RNA by SHAPE. In: Dengue: Protocols and Methods (R. Padebanemabhan, ed.),, Methods in Molecular Biology.
New York/Germany: Springer Publishing; 2014. In Press. [Book Chapter]
13)  Miller J, Le Grice S.
Hope T, Richman D, Stevenson M, eds.
Reverse transcription. In: Encyclopedia of AIDS.
New York: Springer; 2014. In Press. [Book Chapter]
14)  Masaoka T, Chung S, Caboni P, Rausch JW, Wilson JA, Taskent-Sezgin H, Beutler JA, Tocco G, Le Grice S.
Exploiting Drug-Resistant Enzymes as Tools to Identify Thienopyrimidinone Inhibitors of Human Immunodeficiency Virus Reverse Transcriptase-Associated Ribonuclease H.
J. Med. Chem. 2013.
In Press. [Journal]
15)  Le Grice S, Goette M, eds.
Human Immunodeficiency Virus Reverse Transcriptase: A Bench-to-Bedside Success.
New York: Springer Publishing; 2013. [Book (Editor)]
16)  Le Grice S.
(Le Grice S, Goette M, eds.
Conformational dynamics of reverse transcription. In: Human Immunodeficiency Virus Reverse Transcriptase: A Bench-to-Bedside Success.
New York: Springer Publishing; 2013. p. 77-95 [Book Chapter]
17)  Rausch J.
(Le Grice S, Goette M, eds.
Progress towards developing potent and specific inhibitors of HIV RT-associated ribonuclease H. In: Human Immunodeficiency Virus Reverse Transcriptase: A Bench-to-Bedside Success.
New York: Springer Publishing; 2013. p. 143-172 [Book Chapter]
18)  Chung S, Miller JT, Lapkouski M, Tian L, Yang W, Le Grice SF.
Examining the Role of the HIV-1 Reverse Transcriptase p51 Subunit in Positioning and Hydrolysis of RNA/DNA Hybrids.
J. Biol. Chem. 288: 16177-84, 2013.
19)  Purzycka KJ, Legiewicz M, Matsuda E, Eizentstat LD, Lusvarghi S, Saha A, Grice SF, Garfinkel DJ.
Exploring Ty1 retrotransposon RNA structure within virus-like particles.
Nucleic Acids Res. 41: 463-473, 2013.
20)  Kenyon J, Prestwood L, Le Grice S, Lever A.
In-gel probing of individual RNA conformers within a mixed population reveals a dimerization structural switch in the HIV-1 leader.
Nucleic Acids Res. 41 (18): e174, 2013.
Full Text Article. [Journal]
21)  Purzycka KJ, Garfinkel DJ, Boeke JD, Le Grice SF.
Influence of RNA structural elements on Ty1 retrotransposition.
Mob Genet Elements. 3: e25060, 2013.
22)  Huang Q, Purzycka KJ, Lusvarghi S, Li D, Legrice SF, Boeke JD.
Retrotransposon Ty1 RNA contains a 5"-terminal long-range pseudoknot required for efficient reverse transcription.
RNA. 19: 320-32, 2013.
23)  Lusvarghi S, Sztuba-Solinska J, Purzycka K, Rausch J, Le Grice S.
RNA secondary structure prediction using high-throughput SHAPE.
J Vis Exp. e50243, 2013.
Full Text Article. [Journal]
24)  Nowak E, Potrzebowski W, Konarev PV, Rausch JW, Bona MK, Svergun DI, Bujnicki JM, Le Grice SF, Nowotny M.
Structural analysis of monomeric retroviral reverse transcriptase in complex with an RNA/DNA hybrid.
Nucleic Acids Res. 41: 3874-3887, 2013.
Full Text Article. [Journal]
25)  Zhou D, Chung S, Miller M, Le Grice SF, Wlodawer A.
Crystal structures of the reverse transcriptase-associated ribonuclease H domain of xenotropic murine leukemia-virus related virus.
J. Struct. Biol. 177: 638-45, 2012.
26)  Le Grice SF.
Human immunodeficiency virus reverse transcriptase: 25 years of research, drug discovery, and promise.
J. Biol. Chem. 287: 40850-7, 2012.
27)  Barrioluengo V, Wang Y, Le Grice SF, Menéndez-Arias L.
Intrinsic DNA synthesis fidelity of xenotropic murine leukemia virus-related virus reverse transcriptase.
FEBS J. 279: 1433-44, 2012.
28)  Kessl JJ, Jena N, Koh Y, Taskent-Sezgin H, Slaughter A, Feng L, de Silva S, Wu L, Le Grice SF, Engelman A, Fuchs JR, Kvaratskhelia M.
Multimode, Cooperative Mechanism of Action of Allosteric HIV-1 Integrase Inhibitors.
J. Biol. Chem. 287: 16801-11, 2012.
29)  Kearney M, Spindler J, Wiegand A, Shao W, Anderson E, Maldarelli F, Ruscetti F, Mellors J, Hughes S, Le Grice S, Coffin J.
Multiple sources of contamination in samples from patients reported to have XMRV infection.
PLoS ONE. 7(2): e30889, 2012.
Full Text Article. [Journal]
30)  Chung S, Miller JT, Johnson BC, Hughes SH, Le Grice SF.
Mutagenesis of human immunodeficiency virus reverse transcriptase p51 subunit defines residues contributing to vinylogous urea inhibition of ribonuclease H activity.
J Biol Chem. 287: 4066-4075, 2012.
Full Text Article. [Journal]
31)  Sztuba-Solinska J, Le Grice SF.
Probing retroviral and retrotransposon genome structures: The "SHAPE" of things to come.
Mol Biol Int. 2012: 530754, 2012.
32)  Nair GR, Dash C, Le Grice SF, DeStefano JJ.
Viral reverse transcriptases show selective high affinity binding to DNA-DNA primer-templates that resemble the polypurine tract.
PLoS ONE. 7: e41712, 2012.
33)  Chung S, Rausch J, Le Grice S.
Targeting HIV reverse transcriptase-associated ribonuclease H function. In: Proceedings of the 19th International Conference on AIDS, Cancer and Public Health (A. Kozlov, ed.).
St. Petersburg, Russia: .; 2011. p. . [Book Chapter]
34)  Bindewald E, Wendeler M, Legiewicz M, Bona MK, Wang Y, Pritt MJ, Le Grice SF, Shapiro BA.
Correlating SHAPE signatures with three-dimensional RNA structures.
RNA. 17: 1688-96, 2011.
35)  Dash C, Ahmadibeni Y, Hanley MJ, Pandhare J, Gotte M, Le Grice SF, Parang K.
Inhibition of multi-drug resistant HIV-1 reverse transcriptase by nucleoside β-triphosphates.
Bioorg. Med. Chem. Lett. 21: 3519-22, 2011.
36)  Kim JA, Yang SY, Wamiru A, McMahon JB, Le Grice SF, Beutler JA, Kim YH.
New monoterpene glycosides and phenolic compounds from Distylium racemosum and their inhibitory activity against ribonuclease H.
Bioorg. Med. Chem. Lett. 21: 2840-4, 2011.
37)  Kearney M, Lee K, Bagni R, Wiegand A, Spindler J, Maldarelli F, Pinto P, Linehan W, Vocke C, Delviks-Frankenberry K, deVere White R, Del Prete G, Mellors J, Lifson J, KewalRamani V, Pathak V, Coffin J, Le Grice S.
Nucleic acid, antibody, and virus culture methods to detect xenotropic MLV-related virus in human blood samples.
Adv Virol. 2011: article ID 272193, 12 pages: ., 2011.
Full Text Article. [Journal]
38)  Kenyon JC, Tanner SJ, Legiewicz M, Phillip PS, Rizvi TA, Le Grice SF, Lever AM.
SHAPE analysis of the FIV Leader RNA reveals a structural switch potentially controlling viral packaging and genome dimerization.
Nucleic Acids Res. 39: 6692-704, 2011.
39)  Chung S, Himmel DM, Jiang JK, Wojtak K, Bauman JD, Rausch JW, Wilson JA, Beutler JA, Thomas CJ, Arnold E, Le Grice SF.
Synthesis, Activity, and Structural Analysis of Novel α-Hydroxytropolone Inhibitors of Human Immunodeficiency Virus Reverse Transcriptase-Associated Ribonuclease H.
J. Med. Chem. 54: 4462-73, 2011.
40)  Rausch J, Abbondanzieri E, Liu S, Zhuang X, Le Grice S.
Retrovirus replication: New perspectives on enzyme and substrate dynamics. In: Recent Advances in Human Retroviruses: Principles of Replication and Pathogenesis (A.M.L Lever, K.-T. Jeang, and B. Berkhout, eds.).
.: World Scientific Publishing; 2010. p. 307-345 [Book Chapter]
41)  Chung NP, Breun SK, Bashirova A, Baumann JG, Martin TD, Karamchandani JM, Rausch JW, Le Grice SF, Wu L, Carrington M, Kewalramani VN.
HIV-1 transmission by dendritic cell-specific ICAM-3-grabbing nonintegrin (DC-SIGN) is regulated by determinants in the carbohydrate recognition domain that are absent in liver/lymph node-SIGN (L-SIGN).
J. Biol. Chem. 285: 2100-2112, 2010.
42)  Liu S, Harada BT, Miller JT, Le Grice SF, Zhuang X.
Initiation complex dynamics direct the transitions between distinct phases of early HIV reverse transcription.
Nat. Struct. Mol. Biol. 17: 1453-60, 2010.
43)  Goette M, Rausch JW, Marchand B, Sarafianos S, Le Grice SF.
Reverse transcriptase in motion: Conformational dynamics of enzyme-substrate interactions.
Biochim. Biophys. Acta. 1804: 1202-1212, 2010.
44)  Ahmadibeni Y, Dash C, Le Grice SF, Parang K.
Solid-Phase Synthesis of 5'-O-beta,gamma-Methylenetriphosphate Derivatives of Nucleosides and Evaluation of Their Inhibitory Activity Against HIV-1 Reverse Transcriptase.
Tetrahedron letters. 51: 3010-3013, 2010.
45)  Chung S, Wendeler M, Rausch JW, Beilhartz G, Gotte M, O'Keefe BR, Bermingham A, Beutler JA, Liu S, Zhuang X, Le Grice SF.
Structure-Activity Analysis of Vinylogous Urea Inhibitors of Human Immunodeficiency Virus-Encoded Ribonuclease H.
Antimicrobial agents and chemotherapy. 2010.
46)  Ahmadibeni Y, Dash C, Hanley MJ, Le Grice SF, Agarwal HK, Parang K.
Synthesis of nucleoside 5'-O-alpha,beta-methylene-beta-triphosphates and evaluation of their potency towards inhibition of HIV-1 reverse transcriptase.
Org. Biomol. Chem. 8: 1271-4, 2010.
47)  Legiewicz M, Zolotukhin AS, Pilkington GR, Purzycka KJ, Mitchell M, Uranishi H, Bear J, Pavlakis GN, Le Grice SF, Felber BK.
The RNA Transport Element of the Murine musD Retrotransposon Requires Long-range Intramolecular Interactions for Function.
J. Biol. Chem. 285: 42097-104, 2010.
48)  Stoye JP, Silverman RH, Boucher CA, Le Grice SF.
The xenotropic murine leukemia virus-related retrovirus debate continues at first international workshop.
Retrovirology. 7: 113, 2010.
49)  Chung S, Rausch J, Le Grice S.
Targeting HIV-1 reverse transcriptase: A coat with many pockets. In: Innovations in Pharmaceutical Technology. Volume 31.
London, UK: Samedan, Ltd; 2009. p. 48-51 [Book Chapter]
50)  Wendeler M, Miller J, Le Grice S.
Human immunodeficiency virus reverse transcriptase. In: Viral Genome Replication (CE Cameron, M Gotte, KD Raney, eds).
New York: Springer Publications Company; 2009. p. 403-428 [Book Chapter]
51)  Rausch J, Chelico L, Goodman M, Le Grice S.
Dissecting APOBEC3G substrate specificity by nucleoside analog interference.
J Biol Chem. 284: 7047-7058, 2009.
Full Text Article. [Journal]
52)  Beilhartz GL, Wendeler M, Baichoo N, Rausch J, Le Grice S, Götte M.
HIV-1 reverse transcriptase can simultaneously engage its DNA/RNA substrate at both DNA polymerase and RNase H active sites: implications for RNase H inhibition.
J. Mol. Biol. 388: 462-74, 2009.
53)  Wilkinson TA, Januszyk K, Phillips ML, Tekeste SS, Zhang M, Miller JT, Le Grice SF, Clubb RT, Chow SA.
Identifying and characterizing a functional HIV-1 reverse transcriptase-binding site on integrase.
J. Biol. Chem. 284: 7931-7939, 2009.
Full Text Article. [Journal]
54)  Brinson RG, Turner KB, Yi-Brunozzi HY, Le Grice SF, Fabris D, Marino JP.
Probing anomalous structural features in polypurine tract-containing RNA-DNA hybrids with neomycin B.
Biochemistry. 48: 6988-97, 2009.
55)  Fabris D, Marino J, Le Grice S.
Revisiting plus-strand DNA synthesis in retroviruses and long terminal repeat retrotransposons: Dynamics of enzyme:substrate interactions.
Viruses. 1: 657-677, 2009.
Full Text Article. [Journal]
56)  Turner KB, Yi-Brunozzi HY, Brinson RG, Marino JP, Fabris D, Le Grice SF.
SHAMS: Combining chemical modification of RNA with mass spectrometry to examine polypurine tract-containing RNA/DNA hybrids.
RNA. 15: 1605-13, 2009.
57)  Legiewicz M, Rausch J, Le Grice S.
SHAPE technology: A modern framework for RNA structure derivation.
Eur. Pharm. Rev. 3: 13-18, 2009.
58)  Wendeler M, Beilhartz G, Beutler J, Götte M, Le Grice S.
HIV ribonuclease H: Continuing the search for small molecule antagonists.
HIV Ther. 3: 39-53, 2009.
Full Text Article. [Journal]
59)  Liu S, Abbondanzieri EA, Rausch JW, Le Grice SF, Zhuang X.
Slide into action: dynamic shuttling of HIV reverse transcriptase on nucleic acid substrates.
Science. 322: 1092-7, 2008.
60)  Dash C, Scarth BJ, Badorrek C, Götte M, Le Grice SF.
Examining the ribonuclease H primer grip of HIV-1 reverse transcriptase by charge neutralization of RNA/DNA hybrids.
Nucleic Acids Res. 36: 6363-71, 2008.
61)  Ehteshami M, Scarth BJ, Tchesnokov EP, Dash C, Le Grice SF, Hallenberger S, Jochmans D, Götte M.
Mutations M184V and Y115F in HIV-1 reverse transcriptase discriminate against nucleotide-competing reverse transcriptase inhibitors.
J. Biol. Chem. 2008.
62)  Wendeler M, Lee HF, Bermingham A, Miller JT, Chertov O, Bona MK, Baichoo NS, Ehteshami M, Beutler J, O'Keefe BR, Götte M, Kvaratskhelia M, Le Grice S.
Vinylogous ureas as a novel class of inhibitors of reverse transcriptase-associated ribonuclease H activity.
ACS Chem. Biol. 3: 635-44, 2008.
63)  Bokesch HR, Wamiru A, Le Grice SF, Beutler JA, McKee TC, McMahon JB.
HIV-1 ribonuclease H inhibitory phenolic glycosides from Eugenia hyemalis.
J. Nat. Prod. 71: 1634-6, 2008.
64)  Legiewicz M, Badorrek CS, Turner KB, Fabris D, Hamm TE, Rekosh D, Hammarskjöld ML, Le Grice SF.
Resistance to RevM10 inhibition reflects a conformational switch in the HIV-1 Rev response element.
Proc. Natl. Acad. Sci. U.S.A. 105: 14365-70, 2008.
65)  Kvaratskhelia M, LeGrice S.
Structural analysis of protein-RNA interactions with mass spectrometry.
Methods Mol Biol. 488: 213-220, 2008.
66)  Abbondanzieri EA, Bokinsky G, Rausch JW, Zhang JX, Le Grice SF, Zhuang X.
Dynamic binding orientations direct activity of HIV reverse transcriptase.
Nature. 453: 184-9, 2008.
67)  Marchand C, Beutler JA, Wamiru A, Budihas S, Mollmann U, Heinisch L, Mellors JW, Le Grice SF, Pommier Y.
Madurahydroxylactone Derivatives as Dual Inhibitors of Human Immunodeficiency Virus Type 1 Integrase and RNase H.
Antimicrob. Agents Chemother. 52: 361-4, 2008.
68)  Efroni S, Duttagupta R, Cheng J, Dehghani H, Hoeppner DJ, Dash C, Bazett-Jones DP, Le Grice S, McKay RD, Buetow KH, Gingeras TR, Misteli T, Meshorer E.
Global transcription in pluripotent embryonic stem cells.
Cell stem cell. 2: 437-47, 2008.
69)  Yi-Brunozzi HY, Brinson RG, Brabazon DM, Lener D, Le Grice SF, Marino JP.
High-Resolution NMR Analysis of the Conformations of Native and Base Analog Substituted Retroviral and LTR-Retrotransposon PPT Primers.
Chem. Biol. 15: 254-62, 2008.
70)  Turner KB, Brinson RG, Yi-Brunozzi HY, Rausch JW, Miller JT, Le Grice SF, Marino JP, Fabris D.
Structural probing of the HIV-1 polypurine tract RNA:DNA hybrid using classic nucleic acid ligands.
Nucleic Acids Res. 36: 2799-810, 2008.
71)  Klarmann GJ, Eisenhauer BM, Zhang Y, Gotte M, Pata JD, Chatterjee DK, Hecht SM, Le Grice SF.
Investigating the 'steric gate' of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase by targeted insertion of unnatural amino acids.
Biochemistry. 46: 2118-26, 2007.
Full Text Article. [Journal]
72)  Dat NT, Bae K, Wamiru A, McMahon JB, Le Grice SF, Bona M, Beutler JA, Kim YH.
A dimeric lactone from Ardisia japonica with inhibitory activity for HIV-1 and HIV-2 ribonuclease H.
J. Nat. Prod. 70: 839-41, 2007.
73)  Rausch JW, Le Grice SF.
Purine analog substitution of the HIV-1 polypurine tract primer defines regions controlling initiation of plus-strand DNA synthesis.
Nucleic Acids Res. 35: 256-68, 2007.
74)  Rausch JW, Le Grice SF.
Exploiting structurally diverse nucleoside analogs as probes of reverse transcription complexes.
Curr. HIV Res. 5: 11-22, 2007.
75)  Takada K, Bermingham A, O'Keefe BR, Wamiru A, Beutler JA, Le Grice SF, Lloyd J, Gustafson KR, McMahon JB.
An HIV RNase H inhibitory 1,3,4,5-tetragalloylapiitol from the African plant Hylodendron gabunensis.
J. Nat. Prod. 70: 1647-9, 2007.
76)  El Omari K, Ren J, Bird LE, Bona MK, Klarmann G, LeGrice SF, Stammers DK.
Molecular architecture and ligand recognition determinants for T4 RNA ligase.
J Biol Chem. 281: 1573-9, 2006.
77)  Dash C, Fisher TS, Prasad VR, Le Grice SF.
Examining interactions of HIV-1 reverse transcriptase with single-stranded template nucleotides by nucleoside analog interference.
J. Biol. Chem. 281: 27873-81, 2006.
78)  Mandal D, Dash C, Le Grice SF, Prasad VR.
Analysis of HIV-1 replication block due to substitutions at F61 residue of reverse transcriptase reveals additional defects involving the RNase H function.
Nucleic Acids Res. 34: 2853-63, 2006.
79)  Dash C, Marino JP, Le Grice SF.
Examining ty3 polypurine tract structure and function by nucleoside analog interference.
J Biol Chem. 281: 2773-83, 2006.
80)  Bampi C, Bibillo A, Wendeler M, Divita G, Gorelick RJ, Le Grice SF, Darlix JL.
Nucleotide excision repair and template-independent addition by HIV-1 reverse transcriptase in the presence of nucleocapsid protein.
J Biol Chem. 281: 11736-11743, 2006.
81)  Yi-Brunozzi HY, Le Grice SF.
Investigating HIV-1 polypurine tract geometry via targeted insertion of abasic lesions in the (-)-DNA template and (+)-RNA primer.
J Biol Chem. 280: 20154-62, 2005.
82)  Bibillo A, Lener D, Klarmann GJ, Le Grice SF.
Functional roles of carboxylate residues comprising the DNA polymerase active site triad of Ty3 reverse transcriptase.
Nucleic Acids Res. 33: 171-81, 2005.
83)  Williams KL, Zhang Y, Shkriabai N, Karki RG, Nicklaus MC, Kotrikadze N, Hess S, Le Grice SF, Craigie R, Pathak VK, Kvaratskhelia M.
Mass spectrometric analysis of the HIV-1 integrase-pyridoxal 5'-phosphate complex reveals a new binding site for a nucleotide inhibitor.
J Biol Chem. 280: 7949-55, 2005.
84)  Ignatov ME, Berdis AJ, Le Grice SF, Barkley MD.
Attenuation of DNA replication by HIV-1 reverse transcriptase near the central termination sequence.
Biochemistry. 44: 5346-56, 2005.
85)  Yi-Brunozzi HY, Brabazon DM, Lener D, Le Grice SF, Marino JP.
A Ribose Sugar Conformational Switch in the LTR-Retrotransposon Ty3 Polypurine Tract-Containing RNA/DNA Hybrid.
J Am Chem Soc. 127: 16344-16345, 2005.
86)  Budihas SR, Gorshkova I, Gaidamakov S, Wamiru A, Bona MK, Parniak MA, Crouch RJ, McMahon JB, Beutler JA, Le Grice SF.
Selective inhibition of HIV-1 reverse transcriptase-associated ribonuclease H activity by hydroxylated tropolones.
Nucleic Acids Res. 33: 1249-56, 2005.
87)  Bibillo A, Lener D, Tewari A, Le Grice SF.
Interaction of the Ty3 reverse transcriptase thumb subdomain with template-primer.
J Biol Chem. 280: 30282-90, 2005.
88)  Shkriabai N, Patil SS, Hess S, Budihas SR, Craigie R, Burke TR, Le Grice SF, Kvaratskhelia M.
Identification of an inhibitor-binding site to HIV-1 integrase with affinity acetylation and mass spectrometry.
Proc Natl Acad Sci U S A. 101: 6894-9, 2004.
89)  Chan KC, Budihas SR, Le Grice SF, Parniak MA, Crouch RJ, Gaidamakov SA, Isaaq HJ, Wamiru A, McMahon JB, Beutler JA.
A capillary electrophoretic assay for ribonuclease H activity.
Anal Biochem. 331: 296-302, 2004.
90)  Rausch JW, Le Grice SF.
'Binding, bending and bonding': polypurine tract-primed initiation of plus-strand DNA synthesis in human immunodeficiency virus.
Int J Biochem Cell Biol. 36: 1752-1766, 2004.
91)  Sitaraman K, Esposito D, Klarmann G, Le Grice SF, Hartley JL, Chatterjee DK.
A novel cell-free protein synthesis system.
J Biotechnol. 110: 257-263, 2004.
92)  Klarmann GJ, Eisenhauer BM, Zhang Y, Sitaraman K, Chatterjee DK, Hecht SM, Le Grice SF.
Site- and subunit-specific incorporation of unnatural amino acids into HIV-1 reverse transcriptase.
Protein Expr Purif. 38: 37-44, 2004.
93)  Miller JT, Khvorova A, Scaringe SA, Le Grice SF.
Synthetic tRNALys,3 as the replication primer for the HIV-1HXB2 and HIV-1Mal genomes.
Nucleic Acids Res. 32: 4687-95, 2004.
94)  Dash C, Yi-Brunozzi HY, Le Grice SF.
Two modes of HIV-1 polypurine tract cleavage are affected by introducing locked nucleic acid analogs into the (-) DNA template.
J Biol Chem. 279: 37095-102, 2004.
95)  Dash C, Rausch JW, Le Grice SF.
Using pyrrolo-deoxycytosine to probe RNA/DNA hybrids containing the human immunodeficiency virus type-1 3' polypurine tract.
Nucleic Acids Res. 32: 1539-47, 2004.
96)  Le Grice SF.
"In the beginning": Initiation of minus strand DNA synthesis in retroviruses and LTR-containing retrotransposons.
Biochemistry. 42: 14349-14355, 2003.
97)  Parniak MA, Min KL, Budihas SR, Le Grice SF, Beutler JA.
A fluorescence-based high-throughput screening assay for inhibitors of human immunodeficiency virus-1 reverse transcriptase-associated ribonuclease H activity.
Anal. Biochem. 322: 33-39, 2003.
98)  Rigourd M, Bec G, Bénas P, Le Grice SF, Ehresmann B, Ehresmann C, Marquet R.
Effects of tRNA(3)(Lys) aminoacylation on the initiation of HIV-1 reverse transcription.
Biochimie. 85: 521-5, 2003.
99)  Post K, Guo J, Howard KJ, Powell MD, Miller JT, Hizi A, Le Grice SF, Levin JG.
Human immunodeficiency virus type 2 reverse transcriptase activity in model systems that mimic steps in reverse transcription.
J Virol. 77: 7623-34, 2003.
100)  Rausch JW, Qu J, Yi-Brunozzi HY, Kool ET, Le Grice SF.
Hydrolysis of RNA/DNA hybrids containing nonpolar pyrimidine isosteres defines regions essential for HIV type 1 polypurine tract selection.
Proc Natl Acad Sci U S A. 100: 11279-11284, 2003.
101)  Lener D, Kvaratskhelia M, Le Grice SF.
Nonpolar thymine isosteres in the Ty3 polypurine tract DNA template modulate processing and provide a model for its recognition by Ty3 reverse transcriptase.
J. Biol. Chem. 278: 26526-26532, 2003.
102)  Roda RH, Balakrishnan M, Hanson MN, Wohrl BM, Le Grice SF, Roques BP, Gorelick RJ, Bambara RA.
Role of the reverse transcriptase, nucleocapsid protein, and template structure in the two-step transfer mechanism in retroviral recombination.
J Biol Chem. 278: 31536-31546, 2003.
103)  Rausch JW, Lener D, Miller JT, Julias JG, Hughes SH, Le Grice SF.
Altering the RNase H primer grip of human immunodeficiency virus reverse transcriptase modifies cleavage specificity.
Biochemistry. 41: 4856-65, 2002.
104)  Goldschmidt V, Rigourd M, Ehresmann C, Le Grice SF, Ehresmann B, Marquet R.
Direct and indirect contributions of RNA secondary structure elements to the initiation of HIV-1 reverse transcription.
J Biol Chem. 277: 43233-43242, 2002.
105)  Moscardini M, Pistello M, Bendinelli M, Ficheux D, Miller JT, Gabus C, Le Grice SF, Surewicz WK, Darlix JL.
Functional interactions of nucleocapsid protein of feline immunodeficiency virus and cellular prion protein with the viral RNA.
J Mol Biol. 318: 149-59, 2002.
106)  Kvaratskhelia M, Miller JT, Budihas SR, Pannell LK, Le Grice SF.
Identification of specific HIV-1 reverse transcriptase contacts to the viral RNA:tRNA complex by mass spectrometry and a primary amine selective reagent.
Proc Natl Acad Sci U S A. 99: 15988-93, 2002.
107)  Lener D, Budihas SR, Le Grice SF.
Mutating conserved residues in the ribonuclease H domain of Ty3 reverse transcriptase affects specialized cleavage events.
J Biol Chem. 277: 26486-95, 2002.
108)  Cristofaro JV, Rausch JW, Le Grice SF, DeStefano JJ.
Mutations in the ribonuclease H active site of HIV-RT reveal a role for this site in stabilizing enzyme-primer-template binding.
Biochemistry. 41: 10968-75, 2002.
109)  Kvaratskhelia M, Budihas SR, Le Grice SF.
Pre-existing distortions in nucleic acid structure aid polypurine tract selection by HIV-1 reverse transcriptase.
J Biol Chem. 277: 16689-96, 2002.
110)  Brulé F, Marquet R, Rong L, Wainberg MA, Roques BP, Le Grice SF, Ehresmann B, Ehresmann C.
Structural and functional properties of the HIV-1 RNA-tRNA(Lys)3 primer complex annealed by the nucleocapsid protein: comparison with the heat-annealed complex.
RNA. 8: 8-15, 2002.
111)  Nymark-McMahon MH, Beliakova-Bethell NS, Darlix JL, Le Grice SF, Sandmeyer SB.
Ty3 integrase is required for initiation of reverse transcription.
J Virol. 76: 2804-16, 2002.
112)  Klarmann GJ, Hawkins ME, Le Grice SF.
Uncovering the complexities of retroviral ribonuclease H reveals its potential as a therapeutic target.
AIDS Rev. 4: 183-94, 2002.
113)  Miller JT, Ehresmann B, Hübscher U, Le Grice SF.
A novel interaction of tRNA(Lys,3) with the feline immunodeficiency virus RNA genome governs initiation of minus strand DNA synthesis.
J Biol Chem. 276: 27721-30, 2001.
114)  Miller JT, Rausch JW, Le Grice SF.
Evaluation of retroviral ribonuclease H activity.
Methods Mol Biol. 160: 335-54, 2001.
115)  Gorshkova II, Rausch JW, Le Grice SF, Crouch RJ.
HIV-1 reverse transcriptase interaction with model RNA-DNA duplexes.
Anal Biochem. 291: 198-206, 2001.
116)  Berdis AJ, Stetor SR, LeGrice SF, Barkley MD.
Molecular mechanism of sequence-specific termination of lentiviral replication.
Biochemistry. 40: 12140-9, 2001.
117)  Lanchy JM, Isel C, Keith G, Le Grice SF, Ehresmann C, Ehresmann B, Marquet R.
Dynamics of the HIV-1 reverse transcription complex during initiation of DNA synthesis.
J Biol Chem. 275: 12306-12, 2000.
118)  Brulé F, Bec G, Keith G, Le Grice SF, Roques BP, Ehresmann B, Ehresmann C, Marquet R.
In vitro evidence for the interaction of tRNA(3)(Lys) with U3 during the first strand transfer of HIV-1 reverse transcription.
Nucleic Acids Res. 28: 634-40, 2000.
119)  Rigourd M, Lanchy JM, Le Grice SF, Ehresmann B, Ehresmann C, Marquet R.
Inhibition of the initiation of HIV-1 reverse transcription by 3'-azido-3'-deoxythymidine. Comparison with elongation.
J Biol Chem. 275: 26944-51, 2000.
120)  Rausch JW, Grice MK, Henrietta M, Nymark-McMahon , Miller JT, Le Grice SF.
Interaction of p55 reverse transcriptase from the Saccharomyces cerevisiae retrotransposon Ty3 with conformationally distinct nucleic acid duplexes.
J Biol Chem. 275: 13879-87, 2000.
121)  Cowan JA, Ohyama T, Howard K, Rausch JW, Cowan SM, Le Grice SF.
Metal-ion stoichiometry of the HIV-1 RT ribonuclease H domain: evidence for two mutually exclusive sites leads to new mechanistic insights on metal-mediated hydrolysis in nucleic acid biochemistry.
J Biol Inorg Chem. 5: 67-74, 2000.
122)  Rausch JW, Sathyanarayana BK, Bona MK, Le Grice SF.
Probing contacts between the ribonuclease H domain of HIV-1 reverse transcriptase and nucleic acid by site-specific photocross-linking.
J Biol Chem. 275: 16015-22, 2000.
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