Our Science – Yamaguchi Website
Terry P. Yamaguchi, Ph.D.
|Establishment of the anterior-posterior (AP) axis and gastrulation in the mouse embryo.|
|The dashed line overlying the 5.5 dpc embryo indicates the junction between extraembryonic and embryonic ectoderm (epiblast) cells (orange). The epiblast alone gives rise to all cells of the embryo proper. The extraembryonic regions of 6.0-7.5 dpc embryos are outlined for clarity. Prior to gastrulation (before 6.5 dpc), visceral endoderm (VE) cells underly the adjacent embryonic ectoderm (epiblast) in the distal end of the conceptus. VE cells are extraembryonic and do not directly contribute cells to the embryo. Distal visceral endoderm (DVE) cells move anteriorly (arrow) to form the anterior visceral endoderm (AVE) by 6.0 dpc, where they function to anteriorize the adjacent presumptive neural plate (blue). Gastrulation begins at 6.5 dpc with the formation of the primitive streak (PS, red dots) at the posterior end of the embryo. The curved line indicates the proximal-distal length of the primitive streak, which increases as gastrulation proceeds. Mesoderm (red), definitive endoderm (brown), and axial mesendoderm (black) precursors arise in the streak by 7.0 dpc and undergo coordinated morphogenetic movements (arrows) that leads to their eventual placement in anterior positions. Mesoderm cells move immediately adjacent to the overlying epiblast, while definitive endoderm cells constitute the outer-most layer of the conceptus. Anterior and proximal movement of definitive endoderm cells displaces visceral endoderm to extraembryonic locations. Mesodermal cells arising in the anterior end of the primitive streak at 7.0 dpc are presumptive heart mesoderm (red-green hatching), and move to the anterior-most embryonic region by 7.5 dpc where they will form the heart (green). Anterior definitive endoderm (ADE; pink) gives rise to foregut, while the anterior neural plate (blue) forms the forebrain (Curr Biol 11: R713-24, 2001).|
|Confocal microscopy image of ciliated ventral node epithelial cells in a 2 somite stage (embryonic day 8.5) embryo. The node, or organizer, is an important signaling center during vertebrate development that controls the formation of the Left-Right body axis. Anti-acetylated tubulin stains the cilia (green) found on the apical surface of epithelial cells of the node. The cilia play an essential role in establishing the Left-Right axis but the mechanism remains unknown. Actin filaments (red) are visualized with fluorescently labeled phalloidin, and nuclei (blue) are stained with TOPRO-3.|
|The Yamaguchi Lab, 2004|
|L-R: Tadasuke Tsukiyama, Jaime Greear, Bowlathon trophy, Masa-aki Nakaya, Chizuru Nakaya, Terry Yamaguchi, Kristin Biris, Bill Dunty|
|The Annual Yamaguchi/Lewandoski Bowlathon|
The Yamaguchi Lab emerges victorius once again!
|Happy Yamaguchi Lab, Summer 2005|
|L-R: Jaime Greear, Masa-aki Nakaya, Garrett Dunty, Erynn Layman, Bill Dunty, Kristin Biris, Evan, Logan and Terry Yamaguchi|
|Yamaguchi lab 2009|
|Front row: Bill Dunty, Kenya Lyons, Terry Yamaguchi
Back row: Kristen Biris, Rieko Ajima, Ravi Chalamalasetty
This page was last updated on 4/16/2009.